Micromonas commoda (strain RCC299 / NOUM17 / CCMP2709) (Picoplanktonic green alga)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; Mamiellophyceae; Mamiellales; Mamiellaceae; Micromonas

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10115 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C1ECW3|C1ECW3_MICCC Fe2OG dioxygenase domain-containing protein OS=Micromonas commoda (strain RCC299 / NOUM17 / CCMP2709) OX=296587 GN=MICPUN_61562 PE=4 SV=1
MM1 pKa = 7.51VIGAYY6 pKa = 10.35GDD8 pKa = 3.83DD9 pKa = 4.55DD10 pKa = 4.48KK11 pKa = 12.05GSDD14 pKa = 3.24SGLAYY19 pKa = 10.82VFTRR23 pKa = 11.84NTAGDD28 pKa = 4.02LASGWTQVAKK38 pKa = 9.54LTASDD43 pKa = 4.24GAAGDD48 pKa = 3.55QFGQSVSIDD57 pKa = 3.2GDD59 pKa = 4.19TVVIGAWRR67 pKa = 11.84DD68 pKa = 3.4DD69 pKa = 4.01DD70 pKa = 4.55KK71 pKa = 11.82GSEE74 pKa = 4.09SGSAYY79 pKa = 10.45VFTRR83 pKa = 11.84VTAGDD88 pKa = 4.11LASDD92 pKa = 3.95WTQVAKK98 pKa = 9.54LTASDD103 pKa = 3.88GAGYY107 pKa = 10.47NYY109 pKa = 10.35FGISVSIDD117 pKa = 3.1GDD119 pKa = 4.01TMVIGANYY127 pKa = 10.35DD128 pKa = 3.42DD129 pKa = 5.55DD130 pKa = 5.41NGIEE134 pKa = 4.14SGSAYY139 pKa = 10.66VFTRR143 pKa = 11.84NTAGDD148 pKa = 4.02LASGWTQVAKK158 pKa = 9.54LTASDD163 pKa = 3.77GAHH166 pKa = 6.35YY167 pKa = 11.01DD168 pKa = 3.32NFGYY172 pKa = 10.12SVSIDD177 pKa = 3.19GDD179 pKa = 4.14TVVIGARR186 pKa = 11.84YY187 pKa = 10.25DD188 pKa = 3.55DD189 pKa = 4.96DD190 pKa = 4.87KK191 pKa = 11.79GSNSGSAYY199 pKa = 10.29VFTRR203 pKa = 11.84NTAGDD208 pKa = 4.02LASGWTQVAKK218 pKa = 9.46LTSSDD223 pKa = 2.92GAANDD228 pKa = 3.43RR229 pKa = 11.84FGRR232 pKa = 11.84SVSIDD237 pKa = 3.04GDD239 pKa = 4.19TVVIGAYY246 pKa = 10.29ADD248 pKa = 4.93DD249 pKa = 6.09DD250 pKa = 4.98DD251 pKa = 4.82GTDD254 pKa = 3.13SGSAYY259 pKa = 10.75VFTRR263 pKa = 11.84DD264 pKa = 3.23TAGDD268 pKa = 3.9LASGWTQVAKK278 pKa = 9.55LTADD282 pKa = 3.97DD283 pKa = 4.98GAAGDD288 pKa = 3.73NFGWSVSIDD297 pKa = 3.21GDD299 pKa = 4.16TVVIGSQRR307 pKa = 11.84DD308 pKa = 3.12ADD310 pKa = 3.86NGIEE314 pKa = 4.1SGSAYY319 pKa = 10.7VFTRR323 pKa = 11.84DD324 pKa = 3.23TAGDD328 pKa = 3.92LASSWTQFAKK338 pKa = 9.57LTAYY342 pKa = 10.41DD343 pKa = 3.82GAGYY347 pKa = 10.22DD348 pKa = 3.57YY349 pKa = 10.71FGWSVSIDD357 pKa = 3.54GDD359 pKa = 4.0TMVIGAYY366 pKa = 9.6RR367 pKa = 11.84DD368 pKa = 3.34SHH370 pKa = 6.21IANDD374 pKa = 3.35VGSAYY379 pKa = 10.56VFEE382 pKa = 4.96FALTPGSCSVSAPPRR397 pKa = 11.84NGNGNDD403 pKa = 3.61CVFPLAHH410 pKa = 7.03GEE412 pKa = 4.06ACTPVCDD419 pKa = 4.26HH420 pKa = 6.74GFTAVTASAGCVNGNLFPGEE440 pKa = 4.35CRR442 pKa = 11.84CSRR445 pKa = 11.84GYY447 pKa = 9.44PRR449 pKa = 11.84SYY451 pKa = 10.08HH452 pKa = 6.4GNWPCSIEE460 pKa = 4.51KK461 pKa = 10.49RR462 pKa = 11.84MEE464 pKa = 3.83LL465 pKa = 3.92

Molecular weight: 48.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C1E8X9|C1E8X9_MICCC Patatin OS=Micromonas commoda (strain RCC299 / NOUM17 / CCMP2709) OX=296587 GN=MICPUN_59530 PE=3 SV=1
MM1 pKa = 7.75APPAPPGGWGRR12 pKa = 11.84PVSAPAGTGPPSRR25 pKa = 11.84VGPPGGRR32 pKa = 11.84VGPPGGRR39 pKa = 11.84VGPPGGAPARR49 pKa = 11.84FGPSRR54 pKa = 11.84VGPGRR59 pKa = 11.84QQ60 pKa = 2.98

Molecular weight: 5.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10077

38

10115

4889369

24

14149

483.4

52.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.618 ± 0.054

1.574 ± 0.013

6.465 ± 0.023

6.747 ± 0.032

3.147 ± 0.017

8.819 ± 0.044

2.06 ± 0.011

3.192 ± 0.022

4.441 ± 0.026

7.986 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.197 ± 0.013

2.71 ± 0.013

5.56 ± 0.028

2.579 ± 0.015

7.656 ± 0.038

6.571 ± 0.028

5.13 ± 0.019

7.222 ± 0.022

1.303 ± 0.009

2.021 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski