Kingella oralis ATCC 51147
Average proteome isoelectric point is 7.29
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3160 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C4GFZ2|C4GFZ2_9NEIS DNA-directed RNA polymerase subunit omega OS=Kingella oralis ATCC 51147 OX=629741 GN=rpoZ PE=3 SV=1
MM1 pKa = 7.62 LALTLADD8 pKa = 4.15 ANDD11 pKa = 3.88 FVIEE15 pKa = 4.15 AEE17 pKa = 4.07 LDD19 pKa = 3.43 GAEE22 pKa = 4.3 YY23 pKa = 10.91 ALHH26 pKa = 6.41 FAWNDD31 pKa = 3.33 EE32 pKa = 3.85 NSYY35 pKa = 9.23 WALGLEE41 pKa = 4.32 NADD44 pKa = 2.86 GVMQFEE50 pKa = 4.96 GLRR53 pKa = 11.84 ICPNVDD59 pKa = 2.46 ILAGLRR65 pKa = 11.84 YY66 pKa = 9.71 LDD68 pKa = 3.8 VPQGVLFCDD77 pKa = 4.46 GEE79 pKa = 4.33 PDD81 pKa = 2.93 RR82 pKa = 11.84 MAFVDD87 pKa = 4.56 EE88 pKa = 4.33 RR89 pKa = 11.84 AQMIYY94 pKa = 10.78 AGADD98 pKa = 3.31 DD99 pKa = 4.2 VFVV102 pKa = 3.74
Molecular weight: 11.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.63
IPC_protein 3.592
Toseland 3.376
ProMoST 3.77
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.554
Rodwell 3.427
Grimsley 3.3
Solomon 3.567
Lehninger 3.528
Nozaki 3.719
DTASelect 3.935
Thurlkill 3.452
EMBOSS 3.554
Sillero 3.719
Patrickios 0.769
IPC_peptide 3.567
IPC2_peptide 3.681
IPC2.peptide.svr19 3.683
Protein with the highest isoelectric point:
>tr|C4GEM8|C4GEM8_9NEIS Uncharacterized protein OS=Kingella oralis ATCC 51147 OX=629741 GN=GCWU000324_00115 PE=4 SV=1
MM1 pKa = 6.97 NRR3 pKa = 11.84 ARR5 pKa = 11.84 GRR7 pKa = 11.84 FARR10 pKa = 11.84 IGNGRR15 pKa = 11.84 GFMPRR20 pKa = 11.84 QMLPLLPITSKK31 pKa = 11.08 SPAKK35 pKa = 10.05 RR36 pKa = 11.84 RR37 pKa = 11.84 ARR39 pKa = 11.84 TSPARR44 pKa = 11.84 RR45 pKa = 11.84 LILASQLRR53 pKa = 11.84 FATQAAGG60 pKa = 3.17
Molecular weight: 6.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.618
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.34
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.224
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3160
0
3160
709645
37
2608
224.6
24.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.568 ± 0.068
1.166 ± 0.019
4.794 ± 0.037
5.311 ± 0.058
4.106 ± 0.04
7.044 ± 0.064
2.519 ± 0.03
5.696 ± 0.033
4.897 ± 0.047
10.047 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.446 ± 0.026
4.189 ± 0.047
4.638 ± 0.039
5.19 ± 0.049
5.528 ± 0.043
5.337 ± 0.043
5.212 ± 0.05
6.154 ± 0.057
1.322 ± 0.022
2.837 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here