Laribacter hongkongensis (strain HLHK9)
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3208 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C1D5B9|C1D5B9_LARHH PhbF OS=Laribacter hongkongensis (strain HLHK9) OX=557598 GN=phbF PE=4 SV=1
MM1 pKa = 7.17 NAASEE6 pKa = 4.41 MPLPLNFTDD15 pKa = 3.65 SAVAKK20 pKa = 9.95 VSEE23 pKa = 4.78 LIAEE27 pKa = 4.44 EE28 pKa = 4.47 GNPDD32 pKa = 2.99 LKK34 pKa = 11.14 LRR36 pKa = 11.84 VFVTGGGCSGFQYY49 pKa = 10.94 GFTFDD54 pKa = 4.81 EE55 pKa = 4.53 ITNEE59 pKa = 3.84 DD60 pKa = 3.99 DD61 pKa = 4.61 AVMEE65 pKa = 4.42 KK66 pKa = 10.96 GGVTLLIDD74 pKa = 3.8 PMSYY78 pKa = 10.31 QYY80 pKa = 11.58 LVGAEE85 pKa = 3.7 IDD87 pKa = 3.88 YY88 pKa = 11.15 QEE90 pKa = 4.55 SLEE93 pKa = 4.18 GSQFVIKK100 pKa = 10.6 NPNATSTCGCGSSFSVV116 pKa = 3.54
Molecular weight: 12.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.884
IPC_protein 3.795
Toseland 3.605
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.681
Rodwell 3.63
Grimsley 3.528
Solomon 3.745
Lehninger 3.694
Nozaki 3.897
DTASelect 4.037
Thurlkill 3.656
EMBOSS 3.694
Sillero 3.91
Patrickios 0.769
IPC_peptide 3.745
IPC2_peptide 3.884
IPC2.peptide.svr19 3.818
Protein with the highest isoelectric point:
>tr|C1D6L5|C1D6L5_LARHH Uncharacterized protein OS=Laribacter hongkongensis (strain HLHK9) OX=557598 GN=LHK_01130 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVVKK11 pKa = 10.69 RR12 pKa = 11.84 KK13 pKa = 8.03 RR14 pKa = 11.84 THH16 pKa = 5.74 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MKK23 pKa = 9.02 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVIAARR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.7 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSAA44 pKa = 3.91
Molecular weight: 5.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3208
0
3208
919481
21
2414
286.6
31.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.017 ± 0.056
1.105 ± 0.018
5.42 ± 0.039
5.309 ± 0.045
3.521 ± 0.03
8.17 ± 0.052
2.255 ± 0.023
4.602 ± 0.034
3.137 ± 0.042
11.222 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.536 ± 0.025
2.71 ± 0.031
5.177 ± 0.038
4.036 ± 0.034
7.068 ± 0.053
5.397 ± 0.035
5.102 ± 0.033
7.47 ± 0.044
1.476 ± 0.021
2.268 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here