Phialophora attinorum
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11848 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0N1H9Y1|A0A0N1H9Y1_9EURO BTB domain-containing protein OS=Phialophora attinorum OX=1664694 GN=AB675_10658 PE=4 SV=1
MM1 pKa = 6.9 KK2 pKa = 9.22 TAATAIVLAIWVAAPILAQDD22 pKa = 4.14 IDD24 pKa = 4.22 NNDD27 pKa = 3.5 IPTQCATVCSNIVAVSDD44 pKa = 3.57 DD45 pKa = 3.68 CDD47 pKa = 3.23 RR48 pKa = 11.84 AFDD51 pKa = 4.26 RR52 pKa = 11.84 DD53 pKa = 3.97 SDD55 pKa = 4.09 EE56 pKa = 4.38 LACMCQAEE64 pKa = 5.0 GAQQVIPICAACIAFYY80 pKa = 11.01 DD81 pKa = 4.47 DD82 pKa = 5.92 DD83 pKa = 4.84 EE84 pKa = 6.62 DD85 pKa = 5.38 DD86 pKa = 6.03 RR87 pKa = 11.84 RR88 pKa = 11.84 EE89 pKa = 3.98 QDD91 pKa = 3.01 NDD93 pKa = 3.57 AGDD96 pKa = 3.82 LRR98 pKa = 11.84 RR99 pKa = 11.84 DD100 pKa = 3.72 CNFASTTYY108 pKa = 10.6 DD109 pKa = 3.38 PNVTFSQAAMTAATSLTSSSRR130 pKa = 11.84 GANASSAVGSAATAATSATGSAASEE155 pKa = 3.93 ASSAIGSLTSAAATATATGNTAGGVSVDD183 pKa = 3.57 GGRR186 pKa = 11.84 GAMVAGVGLLAAAAFLL202 pKa = 4.29
Molecular weight: 20.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.718
IPC2_protein 3.706
IPC_protein 3.719
Toseland 3.478
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.719
Rodwell 3.541
Grimsley 3.389
Solomon 3.719
Lehninger 3.681
Nozaki 3.859
DTASelect 4.151
Thurlkill 3.554
EMBOSS 3.719
Sillero 3.846
Patrickios 1.888
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.789
Protein with the highest isoelectric point:
>tr|A0A0N0NJL9|A0A0N0NJL9_9EURO Uncharacterized protein OS=Phialophora attinorum OX=1664694 GN=AB675_3606 PE=4 SV=1
MM1 pKa = 7.56 LGTRR5 pKa = 11.84 RR6 pKa = 11.84 SPRR9 pKa = 11.84 TTRR12 pKa = 11.84 HH13 pKa = 4.23 TTTTTKK19 pKa = 10.1 PSLMSRR25 pKa = 11.84 LTGGRR30 pKa = 11.84 SRR32 pKa = 11.84 KK33 pKa = 6.35 THH35 pKa = 4.85 TVSTTSGPRR44 pKa = 11.84 TTHH47 pKa = 5.26 SRR49 pKa = 11.84 RR50 pKa = 11.84 AAPVHH55 pKa = 4.61 HH56 pKa = 6.18 QRR58 pKa = 11.84 RR59 pKa = 11.84 KK60 pKa = 7.98 TSIGDD65 pKa = 3.66 KK66 pKa = 10.55 VAGALKK72 pKa = 10.14 KK73 pKa = 10.13 VQGSLTHH80 pKa = 6.38 NPAKK84 pKa = 10.26 KK85 pKa = 9.9 AAGTRR90 pKa = 11.84 RR91 pKa = 11.84 MHH93 pKa = 5.68 GTDD96 pKa = 2.87 GRR98 pKa = 11.84 GSRR101 pKa = 11.84 RR102 pKa = 11.84 VYY104 pKa = 10.87
Molecular weight: 11.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.366
IPC2_protein 10.891
IPC_protein 12.354
Toseland 12.515
ProMoST 13.013
Dawson 12.53
Bjellqvist 12.515
Wikipedia 12.998
Rodwell 12.223
Grimsley 12.559
Solomon 13.013
Lehninger 12.91
Nozaki 12.515
DTASelect 12.515
Thurlkill 12.515
EMBOSS 13.013
Sillero 12.515
Patrickios 11.945
IPC_peptide 13.013
IPC2_peptide 12.003
IPC2.peptide.svr19 9.085
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11848
0
11848
5661895
39
6066
477.9
52.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.125 ± 0.018
1.14 ± 0.008
5.854 ± 0.015
5.976 ± 0.022
3.604 ± 0.014
6.966 ± 0.024
2.404 ± 0.011
4.755 ± 0.017
4.75 ± 0.02
8.771 ± 0.026
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.2 ± 0.008
3.583 ± 0.012
5.985 ± 0.022
4.095 ± 0.015
6.088 ± 0.022
8.088 ± 0.03
6.175 ± 0.02
6.199 ± 0.022
1.497 ± 0.009
2.746 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here