Oscillochloris trichoides DG-6

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Chloroflexia; Chloroflexales; Chloroflexineae; Oscillochloridaceae; Oscillochloris; Oscillochloris trichoides

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3230 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E1I9Q3|E1I9Q3_9CHLR Uncharacterized protein OS=Oscillochloris trichoides DG-6 OX=765420 GN=OSCT_0054 PE=4 SV=1
MM1 pKa = 7.72NIGIWLTSAVGAAVLAATLAVAPITTAQQTTPTDD35 pKa = 3.18TSALVLQGQGQGQGQGPHH53 pKa = 6.83GDD55 pKa = 3.97GTCSGDD61 pKa = 3.41NYY63 pKa = 10.75VDD65 pKa = 3.68ADD67 pKa = 3.84GDD69 pKa = 4.6GVCDD73 pKa = 4.84NMGTGQGNGDD83 pKa = 3.9NYY85 pKa = 10.85VDD87 pKa = 3.82ADD89 pKa = 3.84GDD91 pKa = 4.6GVCDD95 pKa = 4.84NMGTGQGNGQGNGQGNGQGNGQGRR119 pKa = 11.84GHH121 pKa = 6.36GRR123 pKa = 11.84GRR125 pKa = 11.84PP126 pKa = 3.37

Molecular weight: 12.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E1IAK7|E1IAK7_9CHLR 3-isopropylmalate dehydratase small subunit OS=Oscillochloris trichoides DG-6 OX=765420 GN=leuD PE=3 SV=1
MM1 pKa = 8.1RR2 pKa = 11.84DD3 pKa = 3.03GTLPVPKK10 pKa = 10.13ASNPPGGLKK19 pKa = 10.15PYY21 pKa = 10.24RR22 pKa = 11.84SAATKK27 pKa = 8.83HH28 pKa = 5.66HH29 pKa = 6.26CRR31 pKa = 11.84VPKK34 pKa = 10.47ASNPPGGLKK43 pKa = 10.03LRR45 pKa = 11.84RR46 pKa = 11.84SGAPEE51 pKa = 3.58QGRR54 pKa = 11.84DD55 pKa = 3.39CSEE58 pKa = 4.02SLKK61 pKa = 10.03SARR64 pKa = 11.84RR65 pKa = 11.84IEE67 pKa = 4.23TTHH70 pKa = 6.81FYY72 pKa = 10.94ASSSIDD78 pKa = 3.56LGSEE82 pKa = 3.95SLKK85 pKa = 9.42SARR88 pKa = 11.84RR89 pKa = 11.84IEE91 pKa = 4.05TRR93 pKa = 11.84QFCARR98 pKa = 11.84VRR100 pKa = 11.84WNGVVPKK107 pKa = 10.5ASNPPGGLKK116 pKa = 10.48LYY118 pKa = 8.49TAYY121 pKa = 9.78PVGMFSQKK129 pKa = 10.11CSEE132 pKa = 4.27SLKK135 pKa = 9.02SARR138 pKa = 11.84RR139 pKa = 11.84IEE141 pKa = 4.35TMPGTQKK148 pKa = 10.18RR149 pKa = 11.84RR150 pKa = 11.84RR151 pKa = 11.84CCEE154 pKa = 3.59RR155 pKa = 11.84SSEE158 pKa = 4.3SLKK161 pKa = 9.78SARR164 pKa = 11.84RR165 pKa = 11.84IEE167 pKa = 4.19TPLAGSS173 pKa = 3.64

Molecular weight: 18.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3230

0

3230

1244527

49

6711

385.3

42.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.39 ± 0.057

0.793 ± 0.013

5.2 ± 0.042

5.517 ± 0.044

3.252 ± 0.026

7.754 ± 0.05

2.282 ± 0.022

5.614 ± 0.038

2.068 ± 0.031

11.442 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.284 ± 0.02

2.823 ± 0.041

5.729 ± 0.039

4.389 ± 0.033

7.048 ± 0.045

5.389 ± 0.031

5.64 ± 0.048

7.196 ± 0.036

1.451 ± 0.02

2.738 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski