Leucobacter sp. wl10

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Leucobacter; unclassified Leucobacter

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3409 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A373DFE1|A0A373DFE1_9MICO Uncharacterized protein OS=Leucobacter sp. wl10 OX=2304677 GN=D1J51_14600 PE=4 SV=1
MM1 pKa = 7.75SIPAGWYY8 pKa = 9.74DD9 pKa = 3.88DD10 pKa = 4.43GSGHH14 pKa = 6.08RR15 pKa = 11.84RR16 pKa = 11.84WWDD19 pKa = 3.27GQQWTNDD26 pKa = 3.48YY27 pKa = 11.24APVQGGVIPAQPAPGADD44 pKa = 3.59SDD46 pKa = 4.05TAAPVISTPAAGRR59 pKa = 11.84VSPVLGLVGLGLAVLGTILACIPAVFGVGAVLLLAGFVVSLVGLFTKK106 pKa = 9.41NTAKK110 pKa = 9.98WPSIVGMILSVVGGVIGSIVLAIVLAANLAGPVLPTAPTDD150 pKa = 3.87APPSTTEE157 pKa = 3.99QPSDD161 pKa = 3.78TPTSDD166 pKa = 3.27PSEE169 pKa = 4.13TRR171 pKa = 11.84PSPEE175 pKa = 4.33EE176 pKa = 3.45IAEE179 pKa = 4.9GYY181 pKa = 11.26AEE183 pKa = 4.23MLLAGGITTYY193 pKa = 11.53EE194 pKa = 4.37DD195 pKa = 3.33MPDD198 pKa = 4.07FYY200 pKa = 10.86PCVGQQLYY208 pKa = 10.97DD209 pKa = 3.48SDD211 pKa = 5.26LSDD214 pKa = 3.18EE215 pKa = 4.21ALRR218 pKa = 11.84LISTGQDD225 pKa = 3.56PPDD228 pKa = 3.8SEE230 pKa = 4.7YY231 pKa = 11.45DD232 pKa = 3.25AASQILQDD240 pKa = 4.25VVFTCDD246 pKa = 3.11PNGRR250 pKa = 11.84GVSS253 pKa = 3.51

Molecular weight: 26.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A373DE37|A0A373DE37_9MICO Histidinol dehydrogenase OS=Leucobacter sp. wl10 OX=2304677 GN=hisD PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.5VHH17 pKa = 5.46GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.46GRR40 pKa = 11.84SKK42 pKa = 11.09LSAA45 pKa = 3.74

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3409

0

3409

1124320

29

1958

329.8

35.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.519 ± 0.064

0.566 ± 0.01

5.645 ± 0.036

6.347 ± 0.043

3.109 ± 0.024

9.289 ± 0.04

1.903 ± 0.022

4.518 ± 0.03

1.82 ± 0.031

10.398 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.847 ± 0.019

1.871 ± 0.02

5.404 ± 0.028

2.809 ± 0.02

8.134 ± 0.051

5.604 ± 0.028

5.41 ± 0.026

8.496 ± 0.035

1.388 ± 0.017

1.923 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski