Mucilaginibacter frigoritolerans

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Mucilaginibacter

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4865 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A562UC91|A0A562UC91_9SPHI NAD(P)-dependent dehydrogenase (Short-subunit alcohol dehydrogenase family) OS=Mucilaginibacter frigoritolerans OX=652788 GN=JN11_00993 PE=4 SV=1
MM1 pKa = 7.49FKK3 pKa = 10.85NIFSFNGRR11 pKa = 11.84IRR13 pKa = 11.84RR14 pKa = 11.84TEE16 pKa = 3.69FGISYY21 pKa = 9.92IIYY24 pKa = 8.49FVVYY28 pKa = 10.08LFLLAVTGMFHH39 pKa = 6.25QNKK42 pKa = 9.25EE43 pKa = 4.06LFFVFIPLVWFLLAQGSKK61 pKa = 9.9RR62 pKa = 11.84CHH64 pKa = 6.71DD65 pKa = 4.22LGKK68 pKa = 10.57SGWWQIIPFYY78 pKa = 10.69VLWLLFQDD86 pKa = 4.33GDD88 pKa = 4.16PNEE91 pKa = 4.21NEE93 pKa = 4.05YY94 pKa = 11.53GEE96 pKa = 4.16NPKK99 pKa = 10.44FKK101 pKa = 10.45EE102 pKa = 4.04VFDD105 pKa = 4.34AEE107 pKa = 4.56SYY109 pKa = 9.94EE110 pKa = 4.79DD111 pKa = 5.14PIAPPIYY118 pKa = 10.4DD119 pKa = 3.42VLAANAAADD128 pKa = 4.18IIDD131 pKa = 4.56DD132 pKa = 3.91AVMMDD137 pKa = 4.08LAANEE142 pKa = 3.97QQAVNDD148 pKa = 4.07SAYY151 pKa = 11.06LSADD155 pKa = 3.9DD156 pKa = 4.53NTQSDD161 pKa = 4.75LDD163 pKa = 4.11NADD166 pKa = 3.88SSDD169 pKa = 3.84DD170 pKa = 3.63SSSYY174 pKa = 11.14DD175 pKa = 3.27SSDD178 pKa = 3.09SSGSDD183 pKa = 3.12DD184 pKa = 3.58SGSYY188 pKa = 10.58DD189 pKa = 3.52SSDD192 pKa = 3.36SSSSDD197 pKa = 3.4DD198 pKa = 4.63GMDD201 pKa = 3.42SFF203 pKa = 5.83

Molecular weight: 22.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A562TWJ7|A0A562TWJ7_9SPHI Uncharacterized protein OS=Mucilaginibacter frigoritolerans OX=652788 GN=JN11_03522 PE=4 SV=1
MM1 pKa = 7.27FRR3 pKa = 11.84LSCWQSPVKK12 pKa = 10.38RR13 pKa = 11.84RR14 pKa = 11.84LTGLKK19 pKa = 8.31TLKK22 pKa = 10.48FQEE25 pKa = 4.23FLLNRR30 pKa = 11.84QCGYY34 pKa = 10.61YY35 pKa = 10.55GRR37 pKa = 11.84LHH39 pKa = 6.11QATPCALEE47 pKa = 3.76YY48 pKa = 10.37RR49 pKa = 11.84VVLIRR54 pKa = 11.84RR55 pKa = 11.84KK56 pKa = 9.57IAINTQEE63 pKa = 3.72VDD65 pKa = 3.56VVKK68 pKa = 10.66VFRR71 pKa = 11.84KK72 pKa = 9.81CLFVVFRR79 pKa = 11.84NLVINGSGMSKK90 pKa = 10.7LIAGSNNILHH100 pKa = 6.24TLFSILKK107 pKa = 8.93QCTFTPNGNITGKK120 pKa = 10.68GNQNSPP126 pKa = 3.48

Molecular weight: 14.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4865

0

4865

1664168

39

4837

342.1

38.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.364 ± 0.035

0.808 ± 0.011

5.396 ± 0.024

5.207 ± 0.036

4.972 ± 0.027

6.732 ± 0.033

1.938 ± 0.016

7.692 ± 0.027

7.006 ± 0.033

9.432 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.189 ± 0.018

5.926 ± 0.03

3.891 ± 0.023

3.711 ± 0.022

3.462 ± 0.022

6.436 ± 0.032

6.052 ± 0.05

6.301 ± 0.022

1.192 ± 0.014

4.292 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski