Knoellia subterranea KCTC 19937

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Intrasporangiaceae; Knoellia; Knoellia subterranea

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3418 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0JJN4|A0A0A0JJN4_9MICO Uncharacterized protein OS=Knoellia subterranea KCTC 19937 OX=1385521 GN=N803_14245 PE=4 SV=1
MM1 pKa = 7.22IAVAALVVGLIAVAISSPAADD22 pKa = 3.33VRR24 pKa = 11.84GHH26 pKa = 4.82VCRR29 pKa = 11.84AVADD33 pKa = 4.01VFNLDD38 pKa = 3.93ASCSGGGDD46 pKa = 3.52DD47 pKa = 6.36GEE49 pKa = 4.87TEE51 pKa = 4.2TGDD54 pKa = 3.39PDD56 pKa = 4.51QEE58 pKa = 4.4FEE60 pKa = 4.14PEE62 pKa = 3.6KK63 pKa = 10.96CKK65 pKa = 10.28IHH67 pKa = 5.54EE68 pKa = 4.58TGDD71 pKa = 3.59EE72 pKa = 4.11YY73 pKa = 11.78SSVIKK78 pKa = 10.49IGFIEE83 pKa = 4.09IGEE86 pKa = 4.15NAGFVVTEE94 pKa = 4.14YY95 pKa = 11.48SDD97 pKa = 3.64GTVTMMATNGSEE109 pKa = 3.61IGATGGFGADD119 pKa = 2.92AAYY122 pKa = 10.55GEE124 pKa = 4.75LEE126 pKa = 4.3ANASVDD132 pKa = 3.86FGGGLKK138 pKa = 9.85FDD140 pKa = 4.89YY141 pKa = 10.93GSTWTFEE148 pKa = 4.31SQEE151 pKa = 3.95QADD154 pKa = 4.3EE155 pKa = 4.27FRR157 pKa = 11.84EE158 pKa = 3.98QLDD161 pKa = 4.69DD162 pKa = 3.84YY163 pKa = 12.03LMDD166 pKa = 3.58QWSMTHH172 pKa = 6.28PVCGPTGMCIPRR184 pKa = 11.84PTTGAEE190 pKa = 4.03PPPVPSTTFGGIALTGEE207 pKa = 4.18VSADD211 pKa = 3.39LGISLTSGTTPLTQAEE227 pKa = 4.37LTQQGVAGSISGDD240 pKa = 3.47AHH242 pKa = 4.53WTVTNDD248 pKa = 3.86NNNTPDD254 pKa = 4.52DD255 pKa = 4.65PSDD258 pKa = 3.49DD259 pKa = 3.25TRR261 pKa = 11.84TYY263 pKa = 8.75VTDD266 pKa = 3.28MSLNSEE272 pKa = 4.14VTGQIGLATGGVGSMVGMSYY292 pKa = 10.45SIKK295 pKa = 10.31KK296 pKa = 10.11DD297 pKa = 2.92ADD299 pKa = 3.51GNIIEE304 pKa = 4.24VQIVSTSQVDD314 pKa = 3.56SSGGAGAEE322 pKa = 4.19GEE324 pKa = 4.51VNQGQGKK331 pKa = 7.83NQNSGGGSVTDD342 pKa = 3.56TTTDD346 pKa = 2.91GDD348 pKa = 4.29VVVTEE353 pKa = 4.23TTLAIDD359 pKa = 4.64PDD361 pKa = 4.39DD362 pKa = 4.28SASQQIVQDD371 pKa = 3.49WMGGDD376 pKa = 3.75GDD378 pKa = 3.86FAYY381 pKa = 9.94PGLIKK386 pKa = 10.83LNMVDD391 pKa = 5.03PSQADD396 pKa = 3.61PNDD399 pKa = 3.73PFAMLLHH406 pKa = 6.43NNATSSTIAYY416 pKa = 9.37DD417 pKa = 3.28HH418 pKa = 6.64VSDD421 pKa = 4.84VEE423 pKa = 4.56GFALNVKK430 pKa = 10.18FGLAFGVDD438 pKa = 4.05FSMEE442 pKa = 3.98NSEE445 pKa = 4.3TTATEE450 pKa = 3.78ATYY453 pKa = 10.84LGAPRR458 pKa = 11.84PDD460 pKa = 3.11GTRR463 pKa = 11.84PVLDD467 pKa = 3.42YY468 pKa = 10.09TEE470 pKa = 4.78CVSS473 pKa = 3.84

Molecular weight: 49.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0JM32|A0A0A0JM32_9MICO GGACT domain-containing protein OS=Knoellia subterranea KCTC 19937 OX=1385521 GN=N803_14590 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3418

0

3418

1069712

29

1816

313.0

33.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.99 ± 0.053

0.639 ± 0.009

6.408 ± 0.037

5.762 ± 0.039

2.827 ± 0.026

9.245 ± 0.038

2.212 ± 0.02

3.896 ± 0.033

2.213 ± 0.035

9.949 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.909 ± 0.019

1.895 ± 0.023

5.447 ± 0.032

2.675 ± 0.023

7.348 ± 0.042

5.619 ± 0.029

6.378 ± 0.035

9.229 ± 0.043

1.562 ± 0.016

1.797 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski