Ahrensia marina
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3161 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M9GNV3|A0A0M9GNV3_9RHOB Nitroreductase OS=Ahrensia marina OX=1514904 GN=SU32_04965 PE=4 SV=1
TT1 pKa = 7.62 IEE3 pKa = 5.19 DD4 pKa = 3.63 GGAVSNGDD12 pKa = 3.45 GVIGNDD18 pKa = 3.2 SDD20 pKa = 4.37 GVGAVTVTGSGSTWDD35 pKa = 3.42 NTSTLRR41 pKa = 11.84 VGNAGTGTLTIADD54 pKa = 4.14 GGAVSVNSGTGTVMIADD71 pKa = 4.34 DD72 pKa = 4.68 AGSAGALNIGDD83 pKa = 4.03 GASAGILNAATVDD96 pKa = 3.91 GGSGAATLNFKK107 pKa = 8.79 HH108 pKa = 6.55 TDD110 pKa = 2.67 TDD112 pKa = 4.12 YY113 pKa = 11.35 FFTNDD118 pKa = 3.13 GTSAGAAIDD127 pKa = 3.37 ITGTVAVNHH136 pKa = 6.34 TATGTTTLTGTNTYY150 pKa = 9.2 TGSTIVNFGTLAVDD164 pKa = 4.41 GGSIAHH170 pKa = 6.64 TSADD174 pKa = 4.15 LIVGGFSSDD183 pKa = 4.92 DD184 pKa = 3.31 GTLTIKK190 pKa = 10.93 NGGSVSNNEE199 pKa = 3.69 AGIGYY204 pKa = 9.51 AAGSEE209 pKa = 4.58 GEE211 pKa = 4.29 VSVTGAGSNWTNSGTLYY228 pKa = 10.81 VGLAGSGTVTIADD241 pKa = 3.97 GGSVSNTAGTVGSASGAIGTVTVTGAGSSWSSSSDD276 pKa = 3.41 LYY278 pKa = 11.25 VGEE281 pKa = 4.82 SGTGTMNVEE290 pKa = 4.59 DD291 pKa = 4.5 GATAFVGDD299 pKa = 4.21 DD300 pKa = 3.38 VVLGFNATGNGTLTVDD316 pKa = 3.7 SVGSEE321 pKa = 4.0 LDD323 pKa = 2.98 ITNAFVVGRR332 pKa = 11.84 AGIGALKK339 pKa = 9.21 VQNGGTLTGNYY350 pKa = 9.58 SYY352 pKa = 11.43 VGNSSGATGTATITGSGSSWSLTGDD377 pKa = 4.2 LYY379 pKa = 10.65 IAEE382 pKa = 4.43 QGTGTMTIADD392 pKa = 4.32 GGTVSNAVSYY402 pKa = 10.48 IGYY405 pKa = 10.3 DD406 pKa = 3.3 SGSDD410 pKa = 3.45 GAVTVTGAGSTWTNNSTLYY429 pKa = 10.76 VGEE432 pKa = 5.16 DD433 pKa = 3.59 GNGTLTISDD442 pKa = 4.28 GGTVSAVGGVEE453 pKa = 3.75 IASASGSSVINIGAAEE469 pKa = 4.14 GNAAVAAGTLDD480 pKa = 3.03 TSAIVFGSGNGEE492 pKa = 3.84 LVFNHH497 pKa = 6.87 TDD499 pKa = 2.9 TAYY502 pKa = 10.74 DD503 pKa = 3.62 FDD505 pKa = 4.18 AAIFGLGSIEE515 pKa = 4.3 HH516 pKa = 6.29 FAGVTNLTADD526 pKa = 3.22 NSGFTGEE533 pKa = 4.52 TVVSGGSLYY542 pKa = 11.37 VNDD545 pKa = 4.79 SIGGTVYY552 pKa = 10.89 VDD554 pKa = 4.31 DD555 pKa = 4.56 GTLGGSGTTGALTVNFGGNLAPGNSIGTLNVASAIFNAGSTYY597 pKa = 9.21 TVEE600 pKa = 4.14 LNNGGFVAGTNNDD613 pKa = 3.73 LLNATGVVTINGGTVNVTSEE633 pKa = 4.0 NGTEE637 pKa = 4.23 DD638 pKa = 3.42 GSTYY642 pKa = 9.46 ATPGTYY648 pKa = 9.84 TIITGGSVTGTFDD661 pKa = 3.71 NVSDD665 pKa = 4.79 DD666 pKa = 3.97 YY667 pKa = 12.1 VFLDD671 pKa = 3.98 FTDD674 pKa = 4.83 SYY676 pKa = 11.86 DD677 pKa = 3.38 ATNVYY682 pKa = 8.31 LTSEE686 pKa = 4.12 QVVFFSDD693 pKa = 3.06 IAEE696 pKa = 4.42 TPNQQAIASPLEE708 pKa = 3.93 ALGSGNTVYY717 pKa = 10.82 DD718 pKa = 3.81 ALVGLVGDD726 pKa = 4.41 EE727 pKa = 4.28 DD728 pKa = 3.99 DD729 pKa = 4.64 ARR731 pKa = 11.84 AAFDD735 pKa = 3.65 SLTGEE740 pKa = 4.87 IYY742 pKa = 10.83 ASAQTALLEE751 pKa = 4.29 DD752 pKa = 3.32 SRR754 pKa = 11.84 LPRR757 pKa = 11.84 EE758 pKa = 3.95 AAMEE762 pKa = 4.9 RR763 pKa = 11.84 IRR765 pKa = 11.84 HH766 pKa = 5.38 AFDD769 pKa = 5.94 GIGTDD774 pKa = 4.07 NSAQTEE780 pKa = 4.13 DD781 pKa = 4.53 RR782 pKa = 11.84 ISEE785 pKa = 4.32 SFGLWSQGFGAWSQWKK801 pKa = 10.1 SDD803 pKa = 3.64 GNAATMDD810 pKa = 3.7 RR811 pKa = 11.84 SIGGLLVGGDD821 pKa = 3.58 TMASDD826 pKa = 3.71 NVRR829 pKa = 11.84 FGMLGGYY836 pKa = 8.95 SRR838 pKa = 11.84 SHH840 pKa = 6.63 LNLDD844 pKa = 3.36 NRR846 pKa = 11.84 LSSGTAEE853 pKa = 4.24 TYY855 pKa = 10.04 TLGVYY860 pKa = 10.43 GGGEE864 pKa = 3.62 WDD866 pKa = 3.66 AFSLKK871 pKa = 10.69 GGIAHH876 pKa = 6.74 SWHH879 pKa = 6.35 NLDD882 pKa = 3.34 TSRR885 pKa = 11.84 SVAFNGFSDD894 pKa = 4.05 ILSASYY900 pKa = 10.55 NARR903 pKa = 11.84 TLQAWGEE910 pKa = 4.12 LAVSFDD916 pKa = 3.33 TDD918 pKa = 2.96 IARR921 pKa = 11.84 FEE923 pKa = 4.11 PFANLTHH930 pKa = 6.37 VNLNIDD936 pKa = 3.84 GFTEE940 pKa = 4.11 TGGAAALTAAPNNTDD955 pKa = 2.92 ATFLTVGLRR964 pKa = 11.84 AEE966 pKa = 4.55 TEE968 pKa = 4.22 VSLGSADD975 pKa = 3.24 VTLRR979 pKa = 11.84 GTVAWQHH986 pKa = 5.92 AFSNVPTSQMSFASGDD1002 pKa = 3.7 SPFTIDD1008 pKa = 5.4 GVPLAQDD1015 pKa = 3.62 SLALGAGFDD1024 pKa = 4.19 VNLTDD1029 pKa = 4.37 SAKK1032 pKa = 10.79 LDD1034 pKa = 3.69 FSYY1037 pKa = 11.05 DD1038 pKa = 3.12 GRR1040 pKa = 11.84 FGSDD1044 pKa = 3.09 VHH1046 pKa = 6.72 DD1047 pKa = 4.67 HH1048 pKa = 6.26 SATVSLNVLFF1058 pKa = 5.17
Molecular weight: 105.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.681
IPC2_protein 3.643
IPC_protein 3.694
Toseland 3.452
ProMoST 3.859
Dawson 3.694
Bjellqvist 3.846
Wikipedia 3.643
Rodwell 3.516
Grimsley 3.363
Solomon 3.694
Lehninger 3.656
Nozaki 3.795
DTASelect 4.088
Thurlkill 3.516
EMBOSS 3.656
Sillero 3.821
Patrickios 1.252
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.73
Protein with the highest isoelectric point:
>tr|A0A0M9GLI9|A0A0M9GLI9_9RHOB HAD family hydrolase OS=Ahrensia marina OX=1514904 GN=SU32_12980 PE=4 SV=1
MM1 pKa = 6.23 STKK4 pKa = 9.65 RR5 pKa = 11.84 TFQPSRR11 pKa = 11.84 LVRR14 pKa = 11.84 ARR16 pKa = 11.84 RR17 pKa = 11.84 HH18 pKa = 4.67 GFRR21 pKa = 11.84 SRR23 pKa = 11.84 MATAAGRR30 pKa = 11.84 NVLARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 AKK39 pKa = 10.17 GRR41 pKa = 11.84 KK42 pKa = 8.79 KK43 pKa = 10.63 LSAA46 pKa = 3.95
Molecular weight: 5.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3161
0
3161
971630
41
1601
307.4
33.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.74 ± 0.049
0.886 ± 0.014
5.952 ± 0.036
6.173 ± 0.039
4.129 ± 0.033
7.982 ± 0.04
1.98 ± 0.024
6.376 ± 0.035
4.73 ± 0.035
9.489 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.833 ± 0.022
3.565 ± 0.023
4.381 ± 0.026
3.268 ± 0.025
5.499 ± 0.042
5.994 ± 0.03
5.377 ± 0.026
6.992 ± 0.031
1.213 ± 0.019
2.442 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here