Streptomyces formicae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8132 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A291Q477|A0A291Q477_9ACTN TIR domain-containing protein OS=Streptomyces formicae OX=1616117 GN=KY5_1256 PE=4 SV=1
MM1 pKa = 7.45RR2 pKa = 11.84HH3 pKa = 5.9DD4 pKa = 4.75AEE6 pKa = 4.69TGSEE10 pKa = 4.06GHH12 pKa = 7.14DD13 pKa = 3.94LLEE16 pKa = 3.98VWIDD20 pKa = 3.41QDD22 pKa = 3.99LCTGDD27 pKa = 5.01GICAQYY33 pKa = 10.9APEE36 pKa = 4.23VFEE39 pKa = 5.9LDD41 pKa = 3.05IDD43 pKa = 3.63GLAYY47 pKa = 10.47VKK49 pKa = 10.9SGDD52 pKa = 4.76DD53 pKa = 3.93EE54 pKa = 5.36LLQAPGATTPVPLPLLRR71 pKa = 11.84DD72 pKa = 3.74VVDD75 pKa = 4.03SAKK78 pKa = 10.21EE79 pKa = 3.92CPGDD83 pKa = 4.18CIHH86 pKa = 6.28VRR88 pKa = 11.84RR89 pKa = 11.84VSDD92 pKa = 3.47SVEE95 pKa = 4.14VYY97 pKa = 10.93GPDD100 pKa = 3.69AEE102 pKa = 4.31

Molecular weight: 11.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A291QK32|A0A291QK32_9ACTN Membrane protein OS=Streptomyces formicae OX=1616117 GN=KY5_6777 PE=4 SV=1
MM1 pKa = 6.97VWWVWLVAVVAVAGVLVGGVLGTQARR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84GGGVIVVRR37 pKa = 11.84RR38 pKa = 11.84GRR40 pKa = 11.84RR41 pKa = 11.84PRR43 pKa = 11.84GGRR46 pKa = 11.84ARR48 pKa = 3.66

Molecular weight: 5.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8132

0

8132

2853658

29

8220

350.9

37.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.713 ± 0.039

0.787 ± 0.007

6.097 ± 0.026

5.748 ± 0.024

2.754 ± 0.015

9.584 ± 0.026

2.313 ± 0.013

3.07 ± 0.019

2.351 ± 0.029

10.395 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.708 ± 0.012

1.675 ± 0.013

6.016 ± 0.026

2.637 ± 0.015

7.98 ± 0.036

5.089 ± 0.019

6.058 ± 0.021

8.459 ± 0.026

1.502 ± 0.012

2.063 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski