Streptomyces peucetius subsp. caesius ATCC 27952
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6389 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D3U2Q2|A0A2D3U2Q2_STRC0 Crp/Fnr family transcriptional regulator OS=Streptomyces peucetius subsp. caesius ATCC 27952 OX=316280 GN=CGZ69_00160 PE=4 SV=1
MM1 pKa = 7.17 TVQHH5 pKa = 6.02 EE6 pKa = 4.7 VPTEE10 pKa = 3.96 GAAEE14 pKa = 3.99 ALEE17 pKa = 4.07 VWIDD21 pKa = 3.41 QDD23 pKa = 3.99 LCTGDD28 pKa = 5.01 GICAQYY34 pKa = 10.9 APEE37 pKa = 4.23 VFEE40 pKa = 5.9 LDD42 pKa = 3.05 IDD44 pKa = 3.63 GLAYY48 pKa = 10.43 VKK50 pKa = 10.69 SGEE53 pKa = 4.59 DD54 pKa = 3.94 EE55 pKa = 4.66 LLQDD59 pKa = 4.73 PGATTPVPLPLLSDD73 pKa = 3.26 IVDD76 pKa = 3.91 SAKK79 pKa = 10.35 EE80 pKa = 3.95 CPGDD84 pKa = 4.18 CIHH87 pKa = 6.52 VRR89 pKa = 11.84 RR90 pKa = 11.84 VSDD93 pKa = 3.27 KK94 pKa = 10.85 VEE96 pKa = 4.17 VYY98 pKa = 10.9 GPDD101 pKa = 3.36 AEE103 pKa = 4.31
Molecular weight: 11.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.783
IPC_protein 3.745
Toseland 3.541
ProMoST 3.884
Dawson 3.732
Bjellqvist 3.935
Wikipedia 3.656
Rodwell 3.579
Grimsley 3.465
Solomon 3.719
Lehninger 3.668
Nozaki 3.859
DTASelect 4.05
Thurlkill 3.605
EMBOSS 3.668
Sillero 3.872
Patrickios 0.896
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.796
Protein with the highest isoelectric point:
>tr|A0A2D3UB19|A0A2D3UB19_STRC0 Histidine kinase OS=Streptomyces peucetius subsp. caesius ATCC 27952 OX=316280 GN=CGZ69_18505 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILANRR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.45 GRR40 pKa = 11.84 ANLSAA45 pKa = 4.66
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.647
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.369
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.218
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6389
0
6389
2050681
32
8264
321.0
34.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.471 ± 0.045
0.824 ± 0.01
5.985 ± 0.026
5.897 ± 0.033
2.727 ± 0.016
9.413 ± 0.037
2.304 ± 0.015
3.217 ± 0.025
2.212 ± 0.027
10.273 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.818 ± 0.013
1.76 ± 0.015
6.078 ± 0.03
2.853 ± 0.018
8.09 ± 0.037
5.112 ± 0.021
5.958 ± 0.024
8.503 ± 0.028
1.485 ± 0.014
2.019 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here