Pelagovum pacificum
Average proteome isoelectric point is 5.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3956 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C5GDV1|A0A5C5GDV1_9RHOB sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC OS=Pelagovum pacificum OX=2588711 GN=ugpC PE=4 SV=1
MM1 pKa = 7.09 KK2 pKa = 9.77 TIALATLAAFAAAPAFAAGLDD23 pKa = 4.27 DD24 pKa = 5.06 PAPMAPPAAPAPAPMMMSSDD44 pKa = 2.67 WSGFYY49 pKa = 10.81 GGASLGYY56 pKa = 10.43 GRR58 pKa = 11.84 VEE60 pKa = 4.11 SDD62 pKa = 4.22 ALADD66 pKa = 3.78 DD67 pKa = 4.31 TNGAVYY73 pKa = 10.0 GLHH76 pKa = 6.58 GGYY79 pKa = 8.06 MHH81 pKa = 7.74 DD82 pKa = 4.34 FGSIVAGGEE91 pKa = 3.3 IDD93 pKa = 3.81 YY94 pKa = 10.94 QATNIEE100 pKa = 4.47 DD101 pKa = 3.64 DD102 pKa = 3.88 TTGVEE107 pKa = 3.98 LDD109 pKa = 3.62 SVSRR113 pKa = 11.84 AKK115 pKa = 10.68 LRR117 pKa = 11.84 LGYY120 pKa = 10.17 DD121 pKa = 2.89 AGNFLPYY128 pKa = 9.27 VTGGWAYY135 pKa = 10.69 AEE137 pKa = 4.09 TSGTLEE143 pKa = 4.58 AEE145 pKa = 4.48 DD146 pKa = 3.96 SGSFAGIGIDD156 pKa = 3.59 YY157 pKa = 10.27 KK158 pKa = 10.32 VTDD161 pKa = 4.96 GIMVGGEE168 pKa = 4.09 VLQHH172 pKa = 5.98 QFDD175 pKa = 4.59 DD176 pKa = 4.73 FDD178 pKa = 6.17 DD179 pKa = 4.39 SGIDD183 pKa = 3.44 VDD185 pKa = 4.29 ATTASARR192 pKa = 11.84 VSFQFF197 pKa = 4.14
Molecular weight: 20.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.686
IPC2_protein 3.706
IPC_protein 3.719
Toseland 3.49
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.694
Rodwell 3.554
Grimsley 3.401
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.126
Thurlkill 3.567
EMBOSS 3.706
Sillero 3.846
Patrickios 1.036
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.753
Protein with the highest isoelectric point:
>tr|A0A5C5GHG5|A0A5C5GHG5_9RHOB Sugar ABC transporter permease OS=Pelagovum pacificum OX=2588711 GN=FHY64_13035 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 VRR12 pKa = 11.84 KK13 pKa = 8.99 NRR15 pKa = 11.84 HH16 pKa = 3.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.44 AGRR28 pKa = 11.84 KK29 pKa = 8.46 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.02 KK41 pKa = 10.61 LSAA44 pKa = 4.03
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3956
0
3956
1240708
18
2905
313.6
34.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.968 ± 0.053
0.838 ± 0.012
6.359 ± 0.038
6.369 ± 0.039
3.65 ± 0.026
8.84 ± 0.042
1.973 ± 0.02
5.021 ± 0.026
2.62 ± 0.03
10.035 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.698 ± 0.021
2.434 ± 0.023
5.228 ± 0.028
2.863 ± 0.021
7.01 ± 0.046
5.34 ± 0.026
5.652 ± 0.023
7.431 ± 0.029
1.459 ± 0.018
2.213 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here