Phytohabitans suffuscus
Average proteome isoelectric point is 6.7
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9401 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6F8YCT6|A0A6F8YCT6_9ACTN Acyl dehydratase OS=Phytohabitans suffuscus OX=624315 GN=Psuf_010840 PE=4 SV=1
MM1 pKa = 7.34 LPVAGALLALGLIAAPAAAAPTGDD25 pKa = 3.26 TTTTFSVLAGTLDD38 pKa = 3.56 VVVPATSTVGTGGAPGTSIAGQLGTITVDD67 pKa = 3.47 DD68 pKa = 3.87 TRR70 pKa = 11.84 AAADD74 pKa = 3.8 ASWVTTVTSTDD85 pKa = 3.74 FTTGAAGASEE95 pKa = 4.64 TVPATDD101 pKa = 3.2 VDD103 pKa = 4.61 YY104 pKa = 11.68 SSGPATATTGNGTFTPSGAAAPIGTGITAFTHH136 pKa = 7.1 AGGTGNNSAAWNPTLTVNVPLANVSGTYY164 pKa = 9.53 TGTVTHH170 pKa = 6.66 SVAA173 pKa = 4.02
Molecular weight: 16.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.719
IPC_protein 3.643
Toseland 3.427
ProMoST 3.884
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.706
Rodwell 3.49
Grimsley 3.35
Solomon 3.643
Lehninger 3.605
Nozaki 3.834
DTASelect 4.113
Thurlkill 3.541
EMBOSS 3.706
Sillero 3.783
Patrickios 1.901
IPC_peptide 3.63
IPC2_peptide 3.745
IPC2.peptide.svr19 3.736
Protein with the highest isoelectric point:
>tr|A0A6F8YZJ9|A0A6F8YZJ9_9ACTN dTDP-4-dehydrorhamnose reductase OS=Phytohabitans suffuscus OX=624315 GN=rfbD PE=3 SV=1
MM1 pKa = 7.51 SLPHH5 pKa = 6.62 SGRR8 pKa = 11.84 SSGKK12 pKa = 8.87 RR13 pKa = 11.84 NPPARR18 pKa = 11.84 PTMATPSATTNRR30 pKa = 11.84 RR31 pKa = 11.84 WIATVKK37 pKa = 10.61 NEE39 pKa = 3.68 TSAAPRR45 pKa = 11.84 LVTAQMPATLTTRR58 pKa = 11.84 HH59 pKa = 5.41 STTGQRR65 pKa = 11.84 RR66 pKa = 11.84 RR67 pKa = 11.84 HH68 pKa = 5.99 HH69 pKa = 6.85 SPAQHH74 pKa = 6.39 SSPTARR80 pKa = 11.84 SIVVRR85 pKa = 11.84 PRR87 pKa = 11.84 STPWSKK93 pKa = 9.51 LTAAMASARR102 pKa = 11.84 ATATRR107 pKa = 11.84 KK108 pKa = 8.37 KK109 pKa = 7.28 TTSTNQSRR117 pKa = 11.84 AVRR120 pKa = 11.84 RR121 pKa = 11.84 AGRR124 pKa = 11.84 RR125 pKa = 11.84 ASGG128 pKa = 3.09
Molecular weight: 13.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.184
IPC_protein 12.793
Toseland 12.954
ProMoST 13.451
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.559
Grimsley 12.998
Solomon 13.451
Lehninger 13.349
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.281
IPC_peptide 13.451
IPC2_peptide 12.442
IPC2.peptide.svr19 9.176
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9401
0
9401
2924729
39
7448
311.1
33.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.102 ± 0.051
0.81 ± 0.008
5.9 ± 0.021
5.06 ± 0.025
2.715 ± 0.013
9.534 ± 0.031
2.147 ± 0.012
3.19 ± 0.02
1.61 ± 0.017
10.141 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.695 ± 0.009
1.768 ± 0.019
6.371 ± 0.025
2.667 ± 0.015
8.604 ± 0.03
5.007 ± 0.02
6.004 ± 0.025
8.948 ± 0.028
1.629 ± 0.012
2.098 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here