Burkholderia phage vB_BceS_AH2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ahduovirus; Burkholderia virus AH2

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I6NP79|I6NP79_9CAUD Uncharacterized protein OS=Burkholderia phage vB_BceS_AH2 OX=1133022 GN=AH2_0007 PE=4 SV=1
MM1 pKa = 6.64QQIDD5 pKa = 4.28FGSIDD10 pKa = 3.98ADD12 pKa = 3.58GDD14 pKa = 3.95GPTIGDD20 pKa = 3.49VMEE23 pKa = 4.24SRR25 pKa = 11.84FARR28 pKa = 11.84EE29 pKa = 4.03IISTTGADD37 pKa = 3.24GFDD40 pKa = 4.14AIEE43 pKa = 3.92ISGCMFVAGDD53 pKa = 4.05CVEE56 pKa = 4.5PCTNPDD62 pKa = 3.38DD63 pKa = 4.14VPAFFSVYY71 pKa = 8.56LHH73 pKa = 5.85YY74 pKa = 11.26AEE76 pKa = 4.53GHH78 pKa = 4.82GHH80 pKa = 5.87GVEE83 pKa = 4.37CVGDD87 pKa = 3.73FATAEE92 pKa = 4.11RR93 pKa = 11.84ARR95 pKa = 11.84AYY97 pKa = 9.89AAQIRR102 pKa = 11.84DD103 pKa = 3.44AFGWSVEE110 pKa = 3.81IDD112 pKa = 2.99RR113 pKa = 11.84TAAA116 pKa = 3.35

Molecular weight: 12.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I6NSR2|I6NSR2_9CAUD Uncharacterized protein OS=Burkholderia phage vB_BceS_AH2 OX=1133022 GN=AH2_00015 PE=4 SV=1
MM1 pKa = 7.47AKK3 pKa = 10.19YY4 pKa = 9.88RR5 pKa = 11.84VTVDD9 pKa = 3.47FSNPVARR16 pKa = 11.84AGLHH20 pKa = 5.21EE21 pKa = 4.39IEE23 pKa = 4.62RR24 pKa = 11.84GGPVRR29 pKa = 11.84MFSTGTLHH37 pKa = 6.7EE38 pKa = 4.58AVAHH42 pKa = 5.85RR43 pKa = 11.84NIALFAAGRR52 pKa = 11.84AMQQGPRR59 pKa = 11.84KK60 pKa = 7.96MQVGQRR66 pKa = 11.84DD67 pKa = 3.64EE68 pKa = 5.43RR69 pKa = 11.84EE70 pKa = 4.06AGPVKK75 pKa = 10.69VVIEE79 pKa = 4.06RR80 pKa = 11.84LQQ82 pKa = 3.24

Molecular weight: 9.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

17789

30

1578

228.1

25.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.142 ± 0.741

0.95 ± 0.132

6.65 ± 0.32

5.852 ± 0.27

3.586 ± 0.152

7.746 ± 0.29

1.816 ± 0.193

5.29 ± 0.184

5.009 ± 0.282

8.027 ± 0.225

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.552 ± 0.105

3.862 ± 0.189

4.756 ± 0.32

3.834 ± 0.441

6.83 ± 0.39

5.318 ± 0.216

5.526 ± 0.31

6.639 ± 0.223

1.833 ± 0.15

2.783 ± 0.214
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski