Plesiomonas shigelloides 302-73

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacterales incertae sedis; Plesiomonas; Plesiomonas shigelloides

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3377 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R8AN18|R8AN18_PLESH Signal peptidase I OS=Plesiomonas shigelloides 302-73 OX=1315976 GN=PLESHI_13871 PE=3 SV=1
MM1 pKa = 7.24SHH3 pKa = 6.64IPSEE7 pKa = 4.01LRR9 pKa = 11.84YY10 pKa = 8.28TDD12 pKa = 2.82THH14 pKa = 5.02EE15 pKa = 4.12WVRR18 pKa = 11.84EE19 pKa = 4.06DD20 pKa = 4.7GNGCYY25 pKa = 8.97TVGITDD31 pKa = 4.03HH32 pKa = 6.73AQALLGDD39 pKa = 4.25MVFIDD44 pKa = 5.67LPDD47 pKa = 4.9PDD49 pKa = 4.35SSVAAGDD56 pKa = 3.71DD57 pKa = 3.8CAVAEE62 pKa = 4.4SVKK65 pKa = 10.54AASDD69 pKa = 3.52IYY71 pKa = 11.28APLAGEE77 pKa = 4.21VITVNEE83 pKa = 4.46DD84 pKa = 3.2LADD87 pKa = 3.68QPEE90 pKa = 4.83LVNSDD95 pKa = 3.81PYY97 pKa = 11.16GDD99 pKa = 3.23GWLFQLRR106 pKa = 11.84IDD108 pKa = 5.27DD109 pKa = 4.2PRR111 pKa = 11.84EE112 pKa = 3.88LDD114 pKa = 3.81DD115 pKa = 5.02LLSAEE120 pKa = 5.15EE121 pKa = 4.08YY122 pKa = 10.32AQLIAAEE129 pKa = 4.97DD130 pKa = 4.07DD131 pKa = 4.1DD132 pKa = 5.28SGEE135 pKa = 4.27DD136 pKa = 3.47EE137 pKa = 4.33EE138 pKa = 5.14

Molecular weight: 15.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R8ASE5|R8ASE5_PLESH Cyclic di-GMP-binding protein OS=Plesiomonas shigelloides 302-73 OX=1315976 GN=PLESHI_06574 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84SRR14 pKa = 11.84THH16 pKa = 6.4GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.48GRR39 pKa = 11.84STLSAA44 pKa = 3.82

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3377

0

3377

1067844

30

2191

316.2

34.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.148 ± 0.053

1.135 ± 0.015

5.17 ± 0.029

5.584 ± 0.043

3.712 ± 0.028

7.09 ± 0.041

2.365 ± 0.022

5.547 ± 0.042

3.925 ± 0.037

11.277 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.663 ± 0.022

3.596 ± 0.031

4.52 ± 0.027

5.2 ± 0.051

5.679 ± 0.043

6.027 ± 0.036

5.204 ± 0.028

6.942 ± 0.039

1.365 ± 0.016

2.851 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski