Leucobacter sp. G161

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Leucobacter; unclassified Leucobacter

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3093 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0W7W994|A0A0W7W994_9MICO Histidine triad nucleotide-binding protein OS=Leucobacter sp. G161 OX=663704 GN=AUL38_01850 PE=4 SV=1
MM1 pKa = 7.46GLALLTGAALALSGCATTGDD21 pKa = 4.31DD22 pKa = 4.71DD23 pKa = 4.91GSPGSAEE30 pKa = 4.48KK31 pKa = 9.79DD32 pKa = 3.29TGPLRR37 pKa = 11.84IGTLLPQSGTLAHH50 pKa = 7.35LIYY53 pKa = 10.57GPQAAVKK60 pKa = 10.25LAMEE64 pKa = 6.04DD65 pKa = 3.23INNAGGVLGQDD76 pKa = 2.71IEE78 pKa = 4.47LVIEE82 pKa = 4.29ANEE85 pKa = 4.17FDD87 pKa = 3.97TTDD90 pKa = 3.14PTIINKK96 pKa = 9.83SVDD99 pKa = 4.29DD100 pKa = 5.23IIAAAPSFVLGAMGTGNTNAAMPRR124 pKa = 11.84LSEE127 pKa = 4.26AGILMGSPSNTGVALSGISDD147 pKa = 3.74SYY149 pKa = 11.41FRR151 pKa = 11.84TIASDD156 pKa = 3.96IIQGRR161 pKa = 11.84ALANLVLHH169 pKa = 7.17DD170 pKa = 3.96GHH172 pKa = 6.51TSVAILAQNNDD183 pKa = 3.62YY184 pKa = 9.14GTGLRR189 pKa = 11.84DD190 pKa = 3.52NFQEE194 pKa = 4.11TLEE197 pKa = 4.27AAGAEE202 pKa = 4.02LVYY205 pKa = 10.49GAEE208 pKa = 4.42GNSEE212 pKa = 4.29EE213 pKa = 4.27FQEE216 pKa = 4.48SQTAFAPEE224 pKa = 3.97VAAVLAQNPEE234 pKa = 3.58ALAIVSYY241 pKa = 10.35EE242 pKa = 3.89EE243 pKa = 3.87AMQIIPEE250 pKa = 4.41LAAQGFDD257 pKa = 3.38LSKK260 pKa = 11.03LYY262 pKa = 10.89LVDD265 pKa = 4.65GNTIPYY271 pKa = 9.74PEE273 pKa = 4.66FDD275 pKa = 3.47AGLLEE280 pKa = 4.74GAQGTTPGRR289 pKa = 11.84ATEE292 pKa = 4.65GEE294 pKa = 4.15FLDD297 pKa = 4.39ALVAADD303 pKa = 4.48SSATQSTNFAPEE315 pKa = 4.02AYY317 pKa = 9.63DD318 pKa = 3.71AVVLVSLAAQKK329 pKa = 11.08GGGTDD334 pKa = 3.35TEE336 pKa = 4.52TIIANLGAVSGADD349 pKa = 3.22GGEE352 pKa = 4.18TCDD355 pKa = 4.01TYY357 pKa = 11.68SDD359 pKa = 4.23CLALLNDD366 pKa = 4.4GQSIHH371 pKa = 5.29YY372 pKa = 9.17QGRR375 pKa = 11.84AGGGPLTDD383 pKa = 3.7GNEE386 pKa = 4.0PSSALIGIYY395 pKa = 9.99RR396 pKa = 11.84YY397 pKa = 10.74DD398 pKa = 3.91DD399 pKa = 3.68TNTPQAVGEE408 pKa = 4.25IEE410 pKa = 4.07GG411 pKa = 3.8

Molecular weight: 41.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0W7W687|A0A0W7W687_9MICO ABC transporter ATP-binding protein OS=Leucobacter sp. G161 OX=663704 GN=AUL38_15050 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.23VHH17 pKa = 5.46GFRR20 pKa = 11.84SRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIITARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.68GRR40 pKa = 11.84TKK42 pKa = 10.85LSAA45 pKa = 3.61

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3093

0

3093

999509

29

2953

323.2

34.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.373 ± 0.065

0.511 ± 0.01

5.586 ± 0.034

6.182 ± 0.047

3.267 ± 0.027

9.051 ± 0.04

1.979 ± 0.023

4.789 ± 0.034

2.229 ± 0.034

10.375 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.924 ± 0.02

2.146 ± 0.023

5.191 ± 0.028

2.995 ± 0.021

6.741 ± 0.046

5.792 ± 0.03

6.134 ± 0.034

8.448 ± 0.041

1.394 ± 0.017

1.893 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski