Golden Marseillevirus
Average proteome isoelectric point is 7.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 296 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D6Y702|A0A1D6Y702_9VIRU Putative nucleoside deaminase OS=Golden Marseillevirus OX=1720526 GN=GMAR_ORF22 PE=4 SV=1
MM1 pKa = 7.08 FAVFVEE7 pKa = 4.76 TCEE10 pKa = 4.54 SSVEE14 pKa = 4.16 TSDD17 pKa = 3.5 CRR19 pKa = 11.84 ATIFEE24 pKa = 4.52 VFPDD28 pKa = 3.77 TSVCRR33 pKa = 11.84 VPILAVFAEE42 pKa = 4.76 TVVSRR47 pKa = 11.84 PEE49 pKa = 3.56 TAVFKK54 pKa = 11.24 EE55 pKa = 3.76 EE56 pKa = 3.78 MLAVFVEE63 pKa = 4.69 TCPCKK68 pKa = 10.81 LEE70 pKa = 3.88 MLEE73 pKa = 4.16 VFEE76 pKa = 4.42 VTAVCNAEE84 pKa = 4.1 MSPEE88 pKa = 4.02 NPDD91 pKa = 3.06 ICDD94 pKa = 3.81 DD95 pKa = 3.86 RR96 pKa = 11.84 SEE98 pKa = 4.31 TSPEE102 pKa = 3.79 RR103 pKa = 11.84 VVMSDD108 pKa = 3.46 VFCATCDD115 pKa = 3.46 EE116 pKa = 4.52 RR117 pKa = 11.84 DD118 pKa = 3.45 ATEE121 pKa = 5.62 PEE123 pKa = 4.53 TAWTT127 pKa = 3.81
Molecular weight: 14.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.794
IPC2_protein 3.948
IPC_protein 3.859
Toseland 3.694
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.681
Rodwell 3.694
Grimsley 3.605
Solomon 3.795
Lehninger 3.745
Nozaki 3.923
DTASelect 4.037
Thurlkill 3.719
EMBOSS 3.694
Sillero 3.961
Patrickios 1.85
IPC_peptide 3.795
IPC2_peptide 3.948
IPC2.peptide.svr19 3.865
Protein with the highest isoelectric point:
>tr|A0A1D6Y740|A0A1D6Y740_9VIRU Uncharacterized protein OS=Golden Marseillevirus OX=1720526 GN=GMAR_ORF71 PE=4 SV=1
MM1 pKa = 7.52 FLRR4 pKa = 11.84 SSSNFCEE11 pKa = 4.29 CRR13 pKa = 11.84 SISIQLCPNSLNVNKK28 pKa = 9.44 TLLRR32 pKa = 11.84 NGFHH36 pKa = 7.56 IFTCRR41 pKa = 11.84 DD42 pKa = 3.12 KK43 pKa = 11.03 IFRR46 pKa = 11.84 TFPFALAKK54 pKa = 9.65 QRR56 pKa = 11.84 HH57 pKa = 5.8 KK58 pKa = 10.31 IWRR61 pKa = 11.84 GQTKK65 pKa = 10.11 YY66 pKa = 9.97 YY67 pKa = 9.78 AHH69 pKa = 7.31 NIFKK73 pKa = 10.51 HH74 pKa = 5.74 KK75 pKa = 10.33 KK76 pKa = 6.27 QLKK79 pKa = 7.51 NGRR82 pKa = 11.84 WTEE85 pKa = 4.12 TQQ87 pKa = 2.96
Molecular weight: 10.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.323
IPC2_protein 9.765
IPC_protein 10.248
Toseland 10.877
ProMoST 10.452
Dawson 10.95
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 11.359
Grimsley 10.965
Solomon 11.038
Lehninger 11.023
Nozaki 10.862
DTASelect 10.584
Thurlkill 10.862
EMBOSS 11.257
Sillero 10.877
Patrickios 11.096
IPC_peptide 11.052
IPC2_peptide 9.706
IPC2.peptide.svr19 8.54
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
296
0
296
73257
51
1292
247.5
28.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.206 ± 0.116
2.633 ± 0.111
4.752 ± 0.093
7.707 ± 0.19
5.75 ± 0.11
5.767 ± 0.126
1.876 ± 0.066
4.856 ± 0.082
8.66 ± 0.216
8.967 ± 0.147
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.125 ± 0.065
3.915 ± 0.101
4.012 ± 0.098
3.843 ± 0.122
5.784 ± 0.126
7.728 ± 0.192
5.253 ± 0.177
6.428 ± 0.108
1.522 ± 0.055
3.217 ± 0.088
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here