Litorivita pollutaquae
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3171 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2V4MUX0|A0A2V4MUX0_9RHOB Uncharacterized protein OS=Litorivita pollutaquae OX=2200892 GN=DI396_08480 PE=4 SV=1
MM1 pKa = 8.12 DD2 pKa = 4.77 AQDD5 pKa = 3.51 SAGNQATQRR14 pKa = 11.84 SFVVTVTDD22 pKa = 3.62 TTPPNAPVIGTMGATPDD39 pKa = 3.57 SRR41 pKa = 11.84 VGLEE45 pKa = 4.04 GTAEE49 pKa = 4.33 PGSAVAITFPDD60 pKa = 4.06 GSTQSVIADD69 pKa = 3.58 AGTGAFTVTSNTPQQSGVVTLVATDD94 pKa = 3.76 AEE96 pKa = 5.01 GNDD99 pKa = 4.14 SVPTTAGFTGDD110 pKa = 4.14 DD111 pKa = 3.91 TPPTILIGALSGPTSGTYY129 pKa = 9.37 IAAITLSEE137 pKa = 4.72 DD138 pKa = 3.33 STDD141 pKa = 4.5 FDD143 pKa = 5.04 AADD146 pKa = 3.69 LTLTNATATLTGSGSDD162 pKa = 3.54 YY163 pKa = 10.81 TATLTPAADD172 pKa = 3.84 GTVSLSVAAGVFTDD186 pKa = 4.59 AAGNSNGASNTVSAVFAGVAPTVSISGAPEE216 pKa = 3.68 ALAGGSSFTVAIAFSEE232 pKa = 4.68 VVTGFVAADD241 pKa = 3.61 ISATNATVTALTGGGVSYY259 pKa = 10.92 VATVHH264 pKa = 6.95 ASGTGDD270 pKa = 3.23 VRR272 pKa = 11.84 VAVPANVAFGATGHH286 pKa = 6.49 GNLASSQVVIADD298 pKa = 3.22 VSVQQTQEE306 pKa = 4.22 HH307 pKa = 6.42 IASYY311 pKa = 8.62 MQTRR315 pKa = 11.84 ANQLISNQPSLMSFLSGEE333 pKa = 4.06 PRR335 pKa = 11.84 GKK337 pKa = 10.77 FNFAVTRR344 pKa = 11.84 GAGSFDD350 pKa = 3.57 VATGATGARR359 pKa = 11.84 YY360 pKa = 8.2 PVWAQANGSWTNDD373 pKa = 2.62 GDD375 pKa = 4.23 SEE377 pKa = 4.58 SEE379 pKa = 4.46 YY380 pKa = 11.33 VFGALGGHH388 pKa = 4.73 QTISEE393 pKa = 4.13 NLLVGAMLQFDD404 pKa = 4.68 HH405 pKa = 7.2 LSEE408 pKa = 5.47 DD409 pKa = 3.43 EE410 pKa = 4.41 GVASVRR416 pKa = 11.84 GTGWMVGPYY425 pKa = 9.53 FVARR429 pKa = 11.84 SATQPLYY436 pKa = 10.91 FEE438 pKa = 5.18 GRR440 pKa = 11.84 LLYY443 pKa = 11.05 GEE445 pKa = 4.48 TSNKK449 pKa = 9.32 ISPFGTYY456 pKa = 9.63 EE457 pKa = 4.1 DD458 pKa = 4.53 SFDD461 pKa = 3.74 TTRR464 pKa = 11.84 LLAQLKK470 pKa = 9.6 VAGEE474 pKa = 4.08 LSYY477 pKa = 11.44 GRR479 pKa = 11.84 TLLTPFMDD487 pKa = 4.11 ASYY490 pKa = 9.26 STDD493 pKa = 3.47 DD494 pKa = 3.1 QHH496 pKa = 9.94 SYY498 pKa = 11.18 VDD500 pKa = 3.75 SLGNTIPGQDD510 pKa = 2.95 IALGQIEE517 pKa = 4.42 IGMDD521 pKa = 3.97 FSMMLPVTTGDD532 pKa = 3.41 LEE534 pKa = 4.27 LWGGISGIWSHH545 pKa = 7.35 IDD547 pKa = 3.08 GSGYY551 pKa = 10.95 ASTVTPDD558 pKa = 3.44 YY559 pKa = 10.22 EE560 pKa = 4.22 GGRR563 pKa = 11.84 ARR565 pKa = 11.84 VEE567 pKa = 3.83 LGINHH572 pKa = 6.26 QDD574 pKa = 3.43 SAGHH578 pKa = 6.2 NFTVGTYY585 pKa = 10.24 YY586 pKa = 10.9 DD587 pKa = 4.23 GIGANGYY594 pKa = 9.24 EE595 pKa = 4.38 SYY597 pKa = 11.06 GLSLGYY603 pKa = 10.59 EE604 pKa = 4.11 MQFF607 pKa = 3.35
Molecular weight: 62.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.91
IPC_protein 3.923
Toseland 3.706
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.062
Wikipedia 3.846
Rodwell 3.757
Grimsley 3.617
Solomon 3.91
Lehninger 3.872
Nozaki 4.024
DTASelect 4.266
Thurlkill 3.757
EMBOSS 3.859
Sillero 4.05
Patrickios 1.113
IPC_peptide 3.91
IPC2_peptide 4.024
IPC2.peptide.svr19 3.919
Protein with the highest isoelectric point:
>tr|A0A2V4MNR1|A0A2V4MNR1_9RHOB Enoyl-CoA hydratase OS=Litorivita pollutaquae OX=2200892 GN=DI396_05095 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 8.96 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.62 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.45 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.431
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3171
0
3171
989163
28
1826
311.9
33.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.632 ± 0.06
0.889 ± 0.013
6.1 ± 0.042
5.883 ± 0.039
3.669 ± 0.026
8.728 ± 0.044
2.068 ± 0.02
5.335 ± 0.029
3.47 ± 0.038
9.677 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.953 ± 0.023
2.666 ± 0.022
4.876 ± 0.028
3.183 ± 0.023
6.466 ± 0.042
5.195 ± 0.027
5.527 ± 0.03
7.128 ± 0.034
1.274 ± 0.018
2.28 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here