Staphylococcus phage StB12

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; unclassified Azeredovirinae

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H9A0X1|H9A0X1_9CAUD Uncharacterized protein OS=Staphylococcus phage StB12 OX=1147042 PE=4 SV=1
MM1 pKa = 7.91EE2 pKa = 5.19IKK4 pKa = 10.35QIYY7 pKa = 9.19FYY9 pKa = 10.99DD10 pKa = 3.81GTPFLVMEE18 pKa = 4.71NKK20 pKa = 10.33DD21 pKa = 3.58GEE23 pKa = 4.43LEE25 pKa = 4.14YY26 pKa = 10.51PEE28 pKa = 4.88EE29 pKa = 4.21QWTDD33 pKa = 2.95IAPPEE38 pKa = 5.01GICSPFHH45 pKa = 6.88FDD47 pKa = 2.91GKK49 pKa = 10.65KK50 pKa = 9.62WIGTSYY56 pKa = 10.97EE57 pKa = 3.94EE58 pKa = 4.8WLEE61 pKa = 3.95QQPKK65 pKa = 10.15FEE67 pKa = 4.4VEE69 pKa = 3.98EE70 pKa = 4.32TPDD73 pKa = 4.02DD74 pKa = 4.36KK75 pKa = 11.6DD76 pKa = 3.75VLIADD81 pKa = 4.43LTLQLMEE88 pKa = 4.3TQNTVVNLQNDD99 pKa = 4.02MANLTLQVLEE109 pKa = 4.43SDD111 pKa = 3.78INAA114 pKa = 4.09

Molecular weight: 13.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H9A0T2|H9A0T2_9CAUD YopX domain-containing protein OS=Staphylococcus phage StB12 OX=1147042 PE=4 SV=1
MM1 pKa = 7.41ILSNTINQRR10 pKa = 11.84YY11 pKa = 9.17RR12 pKa = 11.84YY13 pKa = 7.45ATQGKK18 pKa = 7.3TPTQIQHH25 pKa = 6.1EE26 pKa = 4.23LRR28 pKa = 11.84EE29 pKa = 4.22LGVKK33 pKa = 10.2GFVVKK38 pKa = 8.88VTGSRR43 pKa = 11.84VTMKK47 pKa = 10.47VSEE50 pKa = 4.22CDD52 pKa = 2.67IKK54 pKa = 11.04RR55 pKa = 11.84NRR57 pKa = 11.84EE58 pKa = 3.84CMRR61 pKa = 4.42

Molecular weight: 7.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

13932

25

1165

188.3

21.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.584 ± 0.542

0.596 ± 0.076

6.474 ± 0.369

8.125 ± 0.491

4.249 ± 0.218

5.276 ± 0.339

1.579 ± 0.145

7.056 ± 0.28

9.18 ± 0.524

7.752 ± 0.255

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.62 ± 0.143

6.682 ± 0.297

2.878 ± 0.197

4.063 ± 0.204

4.529 ± 0.237

5.857 ± 0.337

5.807 ± 0.231

6.381 ± 0.207

1.105 ± 0.121

4.206 ± 0.314
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski