Amino acid dipepetide frequency for Staphylococcus phage StB12

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.866AlaAla: 1.866 ± 0.468
0.144AlaCys: 0.144 ± 0.112
3.446AlaAsp: 3.446 ± 0.486
4.809AlaGlu: 4.809 ± 0.646
2.8AlaPhe: 2.8 ± 0.635
3.158AlaGly: 3.158 ± 0.498
0.933AlaHis: 0.933 ± 0.277
4.451AlaIle: 4.451 ± 0.789
5.886AlaLys: 5.886 ± 0.778
4.522AlaLeu: 4.522 ± 0.868
1.507AlaMet: 1.507 ± 0.337
4.666AlaAsn: 4.666 ± 0.59
1.364AlaPro: 1.364 ± 0.293
2.512AlaGln: 2.512 ± 0.631
2.082AlaArg: 2.082 ± 0.303
2.728AlaSer: 2.728 ± 0.603
2.943AlaThr: 2.943 ± 0.436
2.943AlaVal: 2.943 ± 0.445
0.79AlaTrp: 0.79 ± 0.25
2.297AlaTyr: 2.297 ± 0.387
0.0AlaXaa: 0.0 ± 0.0
Cys
0.287CysAla: 0.287 ± 0.149
0.144CysCys: 0.144 ± 0.088
0.431CysAsp: 0.431 ± 0.201
0.287CysGlu: 0.287 ± 0.132
0.215CysPhe: 0.215 ± 0.105
0.215CysGly: 0.215 ± 0.123
0.287CysHis: 0.287 ± 0.15
0.431CysIle: 0.431 ± 0.171
0.431CysLys: 0.431 ± 0.159
0.287CysLeu: 0.287 ± 0.142
0.215CysMet: 0.215 ± 0.123
0.287CysAsn: 0.287 ± 0.134
0.144CysPro: 0.144 ± 0.099
0.287CysGln: 0.287 ± 0.152
0.431CysArg: 0.431 ± 0.154
0.502CysSer: 0.502 ± 0.177
0.287CysThr: 0.287 ± 0.156
0.215CysVal: 0.215 ± 0.13
0.144CysTrp: 0.144 ± 0.102
0.431CysTyr: 0.431 ± 0.154
0.0CysXaa: 0.0 ± 0.0
Asp
3.804AspAla: 3.804 ± 0.761
0.574AspCys: 0.574 ± 0.189
4.666AspAsp: 4.666 ± 0.671
6.46AspGlu: 6.46 ± 0.747
3.015AspPhe: 3.015 ± 0.502
3.876AspGly: 3.876 ± 0.572
0.502AspHis: 0.502 ± 0.198
5.527AspIle: 5.527 ± 0.705
5.097AspLys: 5.097 ± 0.745
4.666AspLeu: 4.666 ± 0.541
2.225AspMet: 2.225 ± 0.325
4.379AspAsn: 4.379 ± 0.493
1.149AspPro: 1.149 ± 0.249
0.861AspGln: 0.861 ± 0.218
2.8AspArg: 2.8 ± 0.482
2.943AspSer: 2.943 ± 0.441
4.092AspThr: 4.092 ± 0.641
4.594AspVal: 4.594 ± 0.529
0.718AspTrp: 0.718 ± 0.211
2.8AspTyr: 2.8 ± 0.385
0.0AspXaa: 0.0 ± 0.0
Glu
4.235GluAla: 4.235 ± 0.76
0.646GluCys: 0.646 ± 0.273
4.666GluAsp: 4.666 ± 0.533
7.394GluGlu: 7.394 ± 1.129
3.517GluPhe: 3.517 ± 0.399
3.517GluGly: 3.517 ± 0.373
1.795GluHis: 1.795 ± 0.324
5.671GluIle: 5.671 ± 0.745
5.886GluLys: 5.886 ± 0.833
8.183GluLeu: 8.183 ± 0.886
2.656GluMet: 2.656 ± 0.453
4.451GluAsn: 4.451 ± 0.527
2.082GluPro: 2.082 ± 0.368
3.733GluGln: 3.733 ± 0.572
4.235GluArg: 4.235 ± 0.6
2.512GluSer: 2.512 ± 0.348
4.881GluThr: 4.881 ± 0.578
6.102GluVal: 6.102 ± 0.744
1.22GluTrp: 1.22 ± 0.315
3.876GluTyr: 3.876 ± 0.574
0.0GluXaa: 0.0 ± 0.0
Phe
2.441PheAla: 2.441 ± 0.331
0.359PheCys: 0.359 ± 0.148
3.015PheAsp: 3.015 ± 0.376
3.661PheGlu: 3.661 ± 0.498
1.795PhePhe: 1.795 ± 0.312
2.728PheGly: 2.728 ± 0.579
0.502PheHis: 0.502 ± 0.185
3.374PheIle: 3.374 ± 0.4
4.163PheLys: 4.163 ± 0.587
2.369PheLeu: 2.369 ± 0.341
0.79PheMet: 0.79 ± 0.225
3.446PheAsn: 3.446 ± 0.571
0.431PhePro: 0.431 ± 0.21
0.861PheGln: 0.861 ± 0.255
1.22PheArg: 1.22 ± 0.274
2.943PheSer: 2.943 ± 0.524
2.728PheThr: 2.728 ± 0.41
2.871PheVal: 2.871 ± 0.473
0.431PheTrp: 0.431 ± 0.175
2.369PheTyr: 2.369 ± 0.37
0.0PheXaa: 0.0 ± 0.0
Gly
2.584GlyAla: 2.584 ± 0.701
0.215GlyCys: 0.215 ± 0.114
2.8GlyAsp: 2.8 ± 0.483
2.943GlyGlu: 2.943 ± 0.541
2.369GlyPhe: 2.369 ± 0.391
2.8GlyGly: 2.8 ± 0.55
0.861GlyHis: 0.861 ± 0.342
4.307GlyIle: 4.307 ± 0.518
4.451GlyLys: 4.451 ± 0.571
3.876GlyLeu: 3.876 ± 0.579
1.651GlyMet: 1.651 ± 0.444
3.446GlyAsn: 3.446 ± 0.52
1.077GlyPro: 1.077 ± 0.562
2.8GlyGln: 2.8 ± 0.361
2.369GlyArg: 2.369 ± 0.3
3.589GlySer: 3.589 ± 0.621
3.015GlyThr: 3.015 ± 0.354
4.235GlyVal: 4.235 ± 0.605
0.79GlyTrp: 0.79 ± 0.259
2.584GlyTyr: 2.584 ± 0.441
0.0GlyXaa: 0.0 ± 0.0
His
1.149HisAla: 1.149 ± 0.31
0.0HisCys: 0.0 ± 0.0
0.718HisAsp: 0.718 ± 0.211
0.861HisGlu: 0.861 ± 0.279
0.861HisPhe: 0.861 ± 0.247
1.22HisGly: 1.22 ± 0.299
0.646HisHis: 0.646 ± 0.228
0.861HisIle: 0.861 ± 0.234
1.436HisLys: 1.436 ± 0.295
1.292HisLeu: 1.292 ± 0.306
0.287HisMet: 0.287 ± 0.135
0.861HisAsn: 0.861 ± 0.24
0.287HisPro: 0.287 ± 0.142
0.574HisGln: 0.574 ± 0.219
0.718HisArg: 0.718 ± 0.236
1.005HisSer: 1.005 ± 0.325
0.79HisThr: 0.79 ± 0.24
1.22HisVal: 1.22 ± 0.367
0.072HisTrp: 0.072 ± 0.071
0.933HisTyr: 0.933 ± 0.25
0.0HisXaa: 0.0 ± 0.0
Ile
4.666IleAla: 4.666 ± 0.551
0.502IleCys: 0.502 ± 0.181
5.384IleAsp: 5.384 ± 0.835
6.819IleGlu: 6.819 ± 0.607
2.728IlePhe: 2.728 ± 0.64
3.517IleGly: 3.517 ± 0.564
1.364IleHis: 1.364 ± 0.311
4.163IleIle: 4.163 ± 0.546
7.178IleLys: 7.178 ± 0.72
3.876IleLeu: 3.876 ± 0.6
1.507IleMet: 1.507 ± 0.33
4.953IleAsn: 4.953 ± 0.683
2.8IlePro: 2.8 ± 0.368
2.369IleGln: 2.369 ± 0.354
2.441IleArg: 2.441 ± 0.344
4.02IleSer: 4.02 ± 0.707
4.666IleThr: 4.666 ± 0.562
4.235IleVal: 4.235 ± 0.539
0.861IleTrp: 0.861 ± 0.242
2.441IleTyr: 2.441 ± 0.462
0.0IleXaa: 0.0 ± 0.0
Lys
4.594LysAla: 4.594 ± 0.615
0.144LysCys: 0.144 ± 0.097
6.317LysAsp: 6.317 ± 0.821
9.045LysGlu: 9.045 ± 0.809
3.087LysPhe: 3.087 ± 0.434
4.809LysGly: 4.809 ± 0.699
1.507LysHis: 1.507 ± 0.305
5.384LysIle: 5.384 ± 0.658
8.901LysLys: 8.901 ± 1.184
7.681LysLeu: 7.681 ± 0.786
2.871LysMet: 2.871 ± 0.424
4.092LysAsn: 4.092 ± 0.602
3.446LysPro: 3.446 ± 0.701
4.02LysGln: 4.02 ± 0.512
6.245LysArg: 6.245 ± 0.737
5.025LysSer: 5.025 ± 0.567
4.235LysThr: 4.235 ± 0.653
5.743LysVal: 5.743 ± 0.789
0.861LysTrp: 0.861 ± 0.238
3.804LysTyr: 3.804 ± 0.595
0.0LysXaa: 0.0 ± 0.0
Leu
5.599LeuAla: 5.599 ± 0.905
0.431LeuCys: 0.431 ± 0.217
5.168LeuAsp: 5.168 ± 0.518
5.814LeuGlu: 5.814 ± 0.538
2.584LeuPhe: 2.584 ± 0.36
4.666LeuGly: 4.666 ± 0.679
0.861LeuHis: 0.861 ± 0.212
5.958LeuIle: 5.958 ± 0.515
8.327LeuLys: 8.327 ± 0.77
5.814LeuLeu: 5.814 ± 0.624
2.01LeuMet: 2.01 ± 0.444
6.102LeuAsn: 6.102 ± 0.675
2.441LeuPro: 2.441 ± 0.385
3.589LeuGln: 3.589 ± 0.528
2.297LeuArg: 2.297 ± 0.399
4.953LeuSer: 4.953 ± 0.637
3.733LeuThr: 3.733 ± 0.581
3.948LeuVal: 3.948 ± 0.531
0.718LeuTrp: 0.718 ± 0.199
2.512LeuTyr: 2.512 ± 0.482
0.0LeuXaa: 0.0 ± 0.0
Met
2.153MetAla: 2.153 ± 0.513
0.215MetCys: 0.215 ± 0.111
1.507MetAsp: 1.507 ± 0.314
1.723MetGlu: 1.723 ± 0.337
0.718MetPhe: 0.718 ± 0.246
1.22MetGly: 1.22 ± 0.307
0.215MetHis: 0.215 ± 0.14
1.292MetIle: 1.292 ± 0.28
2.369MetLys: 2.369 ± 0.432
2.441MetLeu: 2.441 ± 0.37
0.502MetMet: 0.502 ± 0.161
1.795MetAsn: 1.795 ± 0.35
0.79MetPro: 0.79 ± 0.261
1.077MetGln: 1.077 ± 0.253
1.292MetArg: 1.292 ± 0.276
1.507MetSer: 1.507 ± 0.266
2.441MetThr: 2.441 ± 0.396
0.861MetVal: 0.861 ± 0.232
0.574MetTrp: 0.574 ± 0.2
1.507MetTyr: 1.507 ± 0.443
0.0MetXaa: 0.0 ± 0.0
Asn
3.948AsnAla: 3.948 ± 0.73
0.502AsnCys: 0.502 ± 0.221
4.307AsnAsp: 4.307 ± 0.598
5.455AsnGlu: 5.455 ± 0.682
3.158AsnPhe: 3.158 ± 0.53
4.163AsnGly: 4.163 ± 0.558
0.79AsnHis: 0.79 ± 0.185
4.307AsnIle: 4.307 ± 0.597
5.814AsnLys: 5.814 ± 0.831
5.097AsnLeu: 5.097 ± 0.533
1.436AsnMet: 1.436 ± 0.281
5.025AsnAsn: 5.025 ± 0.646
2.225AsnPro: 2.225 ± 0.352
2.871AsnGln: 2.871 ± 0.371
2.871AsnArg: 2.871 ± 0.425
4.092AsnSer: 4.092 ± 0.572
3.015AsnThr: 3.015 ± 0.524
4.881AsnVal: 4.881 ± 0.612
0.574AsnTrp: 0.574 ± 0.193
2.297AsnTyr: 2.297 ± 0.51
0.0AsnXaa: 0.0 ± 0.0
Pro
1.077ProAla: 1.077 ± 0.289
0.144ProCys: 0.144 ± 0.103
1.507ProAsp: 1.507 ± 0.374
2.153ProGlu: 2.153 ± 0.376
1.866ProPhe: 1.866 ± 0.38
0.79ProGly: 0.79 ± 0.246
0.574ProHis: 0.574 ± 0.193
1.723ProIle: 1.723 ± 0.307
3.158ProLys: 3.158 ± 0.676
2.297ProLeu: 2.297 ± 0.347
0.502ProMet: 0.502 ± 0.167
2.871ProAsn: 2.871 ± 0.519
1.149ProPro: 1.149 ± 0.266
1.292ProGln: 1.292 ± 0.318
1.005ProArg: 1.005 ± 0.272
2.369ProSer: 2.369 ± 0.427
2.225ProThr: 2.225 ± 0.455
1.364ProVal: 1.364 ± 0.31
0.144ProTrp: 0.144 ± 0.089
0.574ProTyr: 0.574 ± 0.209
0.0ProXaa: 0.0 ± 0.0
Gln
2.441GlnAla: 2.441 ± 0.476
0.144GlnCys: 0.144 ± 0.097
2.584GlnAsp: 2.584 ± 0.572
2.225GlnGlu: 2.225 ± 0.428
1.723GlnPhe: 1.723 ± 0.361
2.297GlnGly: 2.297 ± 0.414
1.364GlnHis: 1.364 ± 0.29
2.728GlnIle: 2.728 ± 0.448
3.302GlnLys: 3.302 ± 0.384
3.733GlnLeu: 3.733 ± 0.675
1.364GlnMet: 1.364 ± 0.333
2.225GlnAsn: 2.225 ± 0.339
0.933GlnPro: 0.933 ± 0.231
2.441GlnGln: 2.441 ± 0.489
2.153GlnArg: 2.153 ± 0.284
2.8GlnSer: 2.8 ± 0.427
2.225GlnThr: 2.225 ± 0.412
2.153GlnVal: 2.153 ± 0.437
0.502GlnTrp: 0.502 ± 0.282
1.292GlnTyr: 1.292 ± 0.296
0.0GlnXaa: 0.0 ± 0.0
Arg
2.153ArgAla: 2.153 ± 0.476
0.144ArgCys: 0.144 ± 0.107
2.153ArgAsp: 2.153 ± 0.378
4.163ArgGlu: 4.163 ± 0.5
1.723ArgPhe: 1.723 ± 0.401
2.297ArgGly: 2.297 ± 0.402
0.718ArgHis: 0.718 ± 0.165
2.512ArgIle: 2.512 ± 0.354
4.307ArgLys: 4.307 ± 0.667
4.666ArgLeu: 4.666 ± 0.563
1.077ArgMet: 1.077 ± 0.302
2.871ArgAsn: 2.871 ± 0.426
1.077ArgPro: 1.077 ± 0.287
1.579ArgGln: 1.579 ± 0.313
2.512ArgArg: 2.512 ± 0.621
2.441ArgSer: 2.441 ± 0.379
2.728ArgThr: 2.728 ± 0.352
2.871ArgVal: 2.871 ± 0.438
0.502ArgTrp: 0.502 ± 0.215
2.8ArgTyr: 2.8 ± 0.481
0.0ArgXaa: 0.0 ± 0.0
Ser
2.584SerAla: 2.584 ± 0.657
0.359SerCys: 0.359 ± 0.175
4.809SerAsp: 4.809 ± 0.606
4.163SerGlu: 4.163 ± 0.754
2.369SerPhe: 2.369 ± 0.436
3.374SerGly: 3.374 ± 0.504
0.933SerHis: 0.933 ± 0.344
4.953SerIle: 4.953 ± 0.598
5.384SerLys: 5.384 ± 0.62
4.307SerLeu: 4.307 ± 0.662
0.718SerMet: 0.718 ± 0.183
4.092SerAsn: 4.092 ± 0.501
1.507SerPro: 1.507 ± 0.35
2.8SerGln: 2.8 ± 0.615
2.441SerArg: 2.441 ± 0.419
4.02SerSer: 4.02 ± 0.749
3.948SerThr: 3.948 ± 0.422
3.158SerVal: 3.158 ± 0.861
0.431SerTrp: 0.431 ± 0.176
2.225SerTyr: 2.225 ± 0.512
0.0SerXaa: 0.0 ± 0.0
Thr
3.733ThrAla: 3.733 ± 0.503
0.359ThrCys: 0.359 ± 0.15
3.374ThrAsp: 3.374 ± 0.609
3.804ThrGlu: 3.804 ± 0.669
3.517ThrPhe: 3.517 ± 0.466
2.871ThrGly: 2.871 ± 0.469
0.646ThrHis: 0.646 ± 0.191
4.092ThrIle: 4.092 ± 0.472
5.025ThrLys: 5.025 ± 0.65
5.025ThrLeu: 5.025 ± 0.455
1.364ThrMet: 1.364 ± 0.381
4.307ThrAsn: 4.307 ± 0.607
2.297ThrPro: 2.297 ± 0.39
2.369ThrGln: 2.369 ± 0.393
1.866ThrArg: 1.866 ± 0.376
3.446ThrSer: 3.446 ± 0.399
2.728ThrThr: 2.728 ± 0.46
4.163ThrVal: 4.163 ± 0.508
0.431ThrTrp: 0.431 ± 0.186
2.656ThrTyr: 2.656 ± 0.51
0.0ThrXaa: 0.0 ± 0.0
Val
3.158ValAla: 3.158 ± 0.733
0.359ValCys: 0.359 ± 0.149
4.235ValAsp: 4.235 ± 0.566
6.102ValGlu: 6.102 ± 0.678
2.369ValPhe: 2.369 ± 0.355
2.512ValGly: 2.512 ± 0.421
0.646ValHis: 0.646 ± 0.191
4.594ValIle: 4.594 ± 0.575
5.743ValLys: 5.743 ± 0.798
4.092ValLeu: 4.092 ± 0.539
2.441ValMet: 2.441 ± 0.396
3.517ValAsn: 3.517 ± 0.452
2.082ValPro: 2.082 ± 0.382
2.512ValGln: 2.512 ± 0.469
3.374ValArg: 3.374 ± 0.388
4.666ValSer: 4.666 ± 0.586
3.804ValThr: 3.804 ± 0.494
4.738ValVal: 4.738 ± 0.653
0.574ValTrp: 0.574 ± 0.194
2.297ValTyr: 2.297 ± 0.48
0.0ValXaa: 0.0 ± 0.0
Trp
1.22TrpAla: 1.22 ± 0.296
0.144TrpCys: 0.144 ± 0.09
0.574TrpAsp: 0.574 ± 0.214
0.79TrpGlu: 0.79 ± 0.244
0.502TrpPhe: 0.502 ± 0.193
0.646TrpGly: 0.646 ± 0.204
0.144TrpHis: 0.144 ± 0.1
0.933TrpIle: 0.933 ± 0.328
0.574TrpLys: 0.574 ± 0.224
1.005TrpLeu: 1.005 ± 0.348
0.0TrpMet: 0.0 ± 0.0
0.79TrpAsn: 0.79 ± 0.188
0.215TrpPro: 0.215 ± 0.12
0.215TrpGln: 0.215 ± 0.122
0.718TrpArg: 0.718 ± 0.256
0.574TrpSer: 0.574 ± 0.216
0.718TrpThr: 0.718 ± 0.178
0.861TrpVal: 0.861 ± 0.275
0.072TrpTrp: 0.072 ± 0.073
0.359TrpTyr: 0.359 ± 0.16
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.153TyrAla: 2.153 ± 0.44
0.431TyrCys: 0.431 ± 0.177
3.087TyrAsp: 3.087 ± 0.585
2.584TyrGlu: 2.584 ± 0.574
1.579TyrPhe: 1.579 ± 0.37
2.082TyrGly: 2.082 ± 0.622
0.502TyrHis: 0.502 ± 0.147
3.302TyrIle: 3.302 ± 0.572
4.379TyrLys: 4.379 ± 0.645
2.297TyrLeu: 2.297 ± 0.489
1.005TyrMet: 1.005 ± 0.24
2.656TyrAsn: 2.656 ± 0.447
1.292TyrPro: 1.292 ± 0.338
1.866TyrGln: 1.866 ± 0.29
2.225TyrArg: 2.225 ± 0.431
2.441TyrSer: 2.441 ± 0.377
2.871TyrThr: 2.871 ± 0.391
2.656TyrVal: 2.656 ± 0.515
0.646TyrTrp: 0.646 ± 0.194
2.01TyrTyr: 2.01 ± 0.374
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 74 proteins (13932 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski