Lactobacillus diolivorans DSM 14421
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2962 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R1SE72|A0A0R1SE72_9LACO Integral membrane protein OS=Lactobacillus diolivorans DSM 14421 OX=1423739 GN=FC85_GL001011 PE=4 SV=1
MM1 pKa = 7.1 YY2 pKa = 9.71 WDD4 pKa = 4.28 GTDD7 pKa = 4.21 FDD9 pKa = 4.2 QTPILNNDD17 pKa = 3.03 WTQSLTEE24 pKa = 4.32 IEE26 pKa = 4.07 NLQIFGDD33 pKa = 3.84 EE34 pKa = 4.4 LYY36 pKa = 9.3 ITVTQHH42 pKa = 5.93 QSPTKK47 pKa = 8.48 VTYY50 pKa = 10.35 IEE52 pKa = 4.07
Molecular weight: 6.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.816
IPC2_protein 3.732
IPC_protein 3.567
Toseland 3.401
ProMoST 3.732
Dawson 3.592
Bjellqvist 3.884
Wikipedia 3.567
Rodwell 3.427
Grimsley 3.325
Solomon 3.528
Lehninger 3.478
Nozaki 3.745
DTASelect 3.897
Thurlkill 3.49
EMBOSS 3.567
Sillero 3.706
Patrickios 0.477
IPC_peptide 3.528
IPC2_peptide 3.668
IPC2.peptide.svr19 3.759
Protein with the highest isoelectric point:
>tr|A0A0R1SBY5|A0A0R1SBY5_9LACO ABC superfamily ATP binding cassette transporter membrane protein OS=Lactobacillus diolivorans DSM 14421 OX=1423739 GN=FC85_GL002781 PE=4 SV=1
MM1 pKa = 7.32 NLQFFSHH8 pKa = 6.49 HH9 pKa = 6.55 KK10 pKa = 10.71 GGGSTANGRR19 pKa = 11.84 NSAGRR24 pKa = 11.84 RR25 pKa = 11.84 LGAKK29 pKa = 9.65 AADD32 pKa = 3.96 GSSVHH37 pKa = 6.49 SGSIIYY43 pKa = 9.3 RR44 pKa = 11.84 QRR46 pKa = 11.84 GTHH49 pKa = 6.81 IYY51 pKa = 9.6 PGNNVSRR58 pKa = 11.84 GNDD61 pKa = 3.23 DD62 pKa = 3.48 TLFALVDD69 pKa = 3.65 GVVRR73 pKa = 11.84 FEE75 pKa = 4.16 RR76 pKa = 11.84 KK77 pKa = 9.84 GRR79 pKa = 11.84 DD80 pKa = 3.02 KK81 pKa = 11.03 RR82 pKa = 11.84 QVSVYY87 pKa = 9.84 PVAEE91 pKa = 3.97 TAKK94 pKa = 10.69
Molecular weight: 10.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.326
IPC2_protein 9.838
IPC_protein 10.76
Toseland 10.833
ProMoST 10.672
Dawson 10.921
Bjellqvist 10.657
Wikipedia 11.169
Rodwell 11.096
Grimsley 10.979
Solomon 11.082
Lehninger 11.038
Nozaki 10.804
DTASelect 10.657
Thurlkill 10.833
EMBOSS 11.242
Sillero 10.862
Patrickios 10.862
IPC_peptide 11.082
IPC2_peptide 9.531
IPC2.peptide.svr19 8.538
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2962
0
2962
886848
49
1950
299.4
33.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.564 ± 0.047
0.468 ± 0.012
5.821 ± 0.05
4.592 ± 0.051
4.37 ± 0.038
6.774 ± 0.037
2.242 ± 0.025
7.5 ± 0.047
6.703 ± 0.05
9.433 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.629 ± 0.022
5.245 ± 0.037
3.709 ± 0.027
4.608 ± 0.04
3.969 ± 0.033
6.453 ± 0.04
6.243 ± 0.042
6.941 ± 0.035
1.069 ± 0.016
3.657 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here