Amino acid dipepetide frequency for Lactobacillus diolivorans DSM 14421

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.704AlaAla: 6.704 ± 0.135
0.405AlaCys: 0.405 ± 0.023
4.638AlaAsp: 4.638 ± 0.079
3.367AlaGlu: 3.367 ± 0.074
3.214AlaPhe: 3.214 ± 0.064
5.569AlaGly: 5.569 ± 0.087
1.411AlaHis: 1.411 ± 0.039
5.957AlaIle: 5.957 ± 0.097
5.735AlaLys: 5.735 ± 0.113
6.919AlaLeu: 6.919 ± 0.099
1.947AlaMet: 1.947 ± 0.052
4.03AlaAsn: 4.03 ± 0.071
2.129AlaPro: 2.129 ± 0.052
3.094AlaGln: 3.094 ± 0.072
2.533AlaArg: 2.533 ± 0.054
4.492AlaSer: 4.492 ± 0.077
4.942AlaThr: 4.942 ± 0.105
5.323AlaVal: 5.323 ± 0.082
0.683AlaTrp: 0.683 ± 0.029
2.551AlaTyr: 2.551 ± 0.057
0.0AlaXaa: 0.0 ± 0.0
Cys
0.31CysAla: 0.31 ± 0.018
0.053CysCys: 0.053 ± 0.008
0.255CysAsp: 0.255 ± 0.019
0.203CysGlu: 0.203 ± 0.016
0.232CysPhe: 0.232 ± 0.016
0.474CysGly: 0.474 ± 0.024
0.158CysHis: 0.158 ± 0.013
0.303CysIle: 0.303 ± 0.019
0.127CysLys: 0.127 ± 0.012
0.557CysLeu: 0.557 ± 0.025
0.112CysMet: 0.112 ± 0.012
0.18CysAsn: 0.18 ± 0.014
0.218CysPro: 0.218 ± 0.018
0.211CysGln: 0.211 ± 0.017
0.215CysArg: 0.215 ± 0.016
0.28CysSer: 0.28 ± 0.016
0.226CysThr: 0.226 ± 0.016
0.307CysVal: 0.307 ± 0.018
0.059CysTrp: 0.059 ± 0.008
0.202CysTyr: 0.202 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
4.028AspAla: 4.028 ± 0.091
0.232AspCys: 0.232 ± 0.017
3.733AspAsp: 3.733 ± 0.081
3.439AspGlu: 3.439 ± 0.075
2.681AspPhe: 2.681 ± 0.053
3.653AspGly: 3.653 ± 0.07
1.688AspHis: 1.688 ± 0.044
3.664AspIle: 3.664 ± 0.064
3.657AspLys: 3.657 ± 0.081
5.317AspLeu: 5.317 ± 0.085
1.395AspMet: 1.395 ± 0.046
2.838AspAsn: 2.838 ± 0.066
2.557AspPro: 2.557 ± 0.054
3.474AspGln: 3.474 ± 0.072
2.526AspArg: 2.526 ± 0.054
3.374AspSer: 3.374 ± 0.072
2.787AspThr: 2.787 ± 0.066
3.936AspVal: 3.936 ± 0.07
0.722AspTrp: 0.722 ± 0.026
2.51AspTyr: 2.51 ± 0.054
0.0AspXaa: 0.0 ± 0.0
Glu
3.459GluAla: 3.459 ± 0.066
0.157GluCys: 0.157 ± 0.015
2.385GluAsp: 2.385 ± 0.059
2.248GluGlu: 2.248 ± 0.06
1.824GluPhe: 1.824 ± 0.048
2.28GluGly: 2.28 ± 0.061
0.989GluHis: 0.989 ± 0.033
3.737GluIle: 3.737 ± 0.073
3.708GluLys: 3.708 ± 0.078
4.767GluLeu: 4.767 ± 0.089
1.561GluMet: 1.561 ± 0.045
2.67GluAsn: 2.67 ± 0.053
1.53GluPro: 1.53 ± 0.047
2.189GluGln: 2.189 ± 0.056
1.986GluArg: 1.986 ± 0.055
2.632GluSer: 2.632 ± 0.058
2.821GluThr: 2.821 ± 0.07
3.005GluVal: 3.005 ± 0.068
0.452GluTrp: 0.452 ± 0.025
1.522GluTyr: 1.522 ± 0.042
0.0GluXaa: 0.0 ± 0.0
Phe
3.157PheAla: 3.157 ± 0.062
0.297PheCys: 0.297 ± 0.02
2.933PheAsp: 2.933 ± 0.057
2.112PheGlu: 2.112 ± 0.048
2.003PhePhe: 2.003 ± 0.061
3.508PheGly: 3.508 ± 0.083
0.89PheHis: 0.89 ± 0.027
3.13PheIle: 3.13 ± 0.072
2.764PheLys: 2.764 ± 0.054
3.823PheLeu: 3.823 ± 0.086
1.099PheMet: 1.099 ± 0.041
2.474PheAsn: 2.474 ± 0.059
1.568PhePro: 1.568 ± 0.046
1.496PheGln: 1.496 ± 0.041
1.384PheArg: 1.384 ± 0.042
3.12PheSer: 3.12 ± 0.063
2.509PheThr: 2.509 ± 0.054
3.171PheVal: 3.171 ± 0.067
0.596PheTrp: 0.596 ± 0.03
1.669PheTyr: 1.669 ± 0.046
0.0PheXaa: 0.0 ± 0.0
Gly
4.641GlyAla: 4.641 ± 0.082
0.397GlyCys: 0.397 ± 0.023
3.445GlyAsp: 3.445 ± 0.079
2.812GlyGlu: 2.812 ± 0.069
3.211GlyPhe: 3.211 ± 0.064
4.32GlyGly: 4.32 ± 0.082
1.535GlyHis: 1.535 ± 0.04
5.971GlyIle: 5.971 ± 0.088
4.813GlyLys: 4.813 ± 0.085
6.645GlyLeu: 6.645 ± 0.092
2.034GlyMet: 2.034 ± 0.054
3.229GlyAsn: 3.229 ± 0.057
1.662GlyPro: 1.662 ± 0.04
2.882GlyGln: 2.882 ± 0.069
2.543GlyArg: 2.543 ± 0.06
4.373GlySer: 4.373 ± 0.081
4.49GlyThr: 4.49 ± 0.089
4.928GlyVal: 4.928 ± 0.082
0.836GlyTrp: 0.836 ± 0.037
2.973GlyTyr: 2.973 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
1.366HisAla: 1.366 ± 0.041
0.136HisCys: 0.136 ± 0.013
1.297HisAsp: 1.297 ± 0.043
1.186HisGlu: 1.186 ± 0.037
1.146HisPhe: 1.146 ± 0.042
1.583HisGly: 1.583 ± 0.048
0.827HisHis: 0.827 ± 0.033
1.431HisIle: 1.431 ± 0.043
1.053HisLys: 1.053 ± 0.037
2.183HisLeu: 2.183 ± 0.047
0.509HisMet: 0.509 ± 0.022
1.029HisAsn: 1.029 ± 0.036
1.141HisPro: 1.141 ± 0.038
1.344HisGln: 1.344 ± 0.044
0.993HisArg: 0.993 ± 0.034
1.283HisSer: 1.283 ± 0.038
1.072HisThr: 1.072 ± 0.038
1.491HisVal: 1.491 ± 0.045
0.289HisTrp: 0.289 ± 0.021
1.062HisTyr: 1.062 ± 0.039
0.0HisXaa: 0.0 ± 0.0
Ile
6.047IleAla: 6.047 ± 0.101
0.483IleCys: 0.483 ± 0.023
4.746IleAsp: 4.746 ± 0.096
3.325IleGlu: 3.325 ± 0.067
3.329IlePhe: 3.329 ± 0.082
5.723IleGly: 5.723 ± 0.095
1.467IleHis: 1.467 ± 0.043
5.641IleIle: 5.641 ± 0.103
4.771IleLys: 4.771 ± 0.068
6.637IleLeu: 6.637 ± 0.115
1.791IleMet: 1.791 ± 0.053
4.152IleAsn: 4.152 ± 0.069
3.011IlePro: 3.011 ± 0.064
2.808IleGln: 2.808 ± 0.063
2.684IleArg: 2.684 ± 0.057
5.359IleSer: 5.359 ± 0.093
4.522IleThr: 4.522 ± 0.065
5.416IleVal: 5.416 ± 0.078
0.762IleTrp: 0.762 ± 0.031
2.33IleTyr: 2.33 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
4.899LysAla: 4.899 ± 0.105
0.164LysCys: 0.164 ± 0.012
3.693LysAsp: 3.693 ± 0.077
3.187LysGlu: 3.187 ± 0.069
2.316LysPhe: 2.316 ± 0.053
3.544LysGly: 3.544 ± 0.067
1.513LysHis: 1.513 ± 0.042
4.726LysIle: 4.726 ± 0.066
5.208LysLys: 5.208 ± 0.082
6.095LysLeu: 6.095 ± 0.085
2.292LysMet: 2.292 ± 0.046
3.713LysAsn: 3.713 ± 0.069
2.404LysPro: 2.404 ± 0.051
3.744LysGln: 3.744 ± 0.066
3.247LysArg: 3.247 ± 0.074
4.175LysSer: 4.175 ± 0.082
4.197LysThr: 4.197 ± 0.077
4.593LysVal: 4.593 ± 0.077
0.71LysTrp: 0.71 ± 0.032
2.608LysTyr: 2.608 ± 0.061
0.0LysXaa: 0.0 ± 0.0
Leu
7.813LeuAla: 7.813 ± 0.109
0.495LeuCys: 0.495 ± 0.024
5.217LeuAsp: 5.217 ± 0.08
3.692LeuGlu: 3.692 ± 0.07
4.121LeuPhe: 4.121 ± 0.083
6.595LeuGly: 6.595 ± 0.102
1.779LeuHis: 1.779 ± 0.047
7.632LeuIle: 7.632 ± 0.133
6.432LeuLys: 6.432 ± 0.087
8.662LeuLeu: 8.662 ± 0.135
2.459LeuMet: 2.459 ± 0.055
5.048LeuAsn: 5.048 ± 0.082
3.942LeuPro: 3.942 ± 0.076
3.321LeuGln: 3.321 ± 0.064
3.41LeuArg: 3.41 ± 0.07
6.969LeuSer: 6.969 ± 0.087
6.731LeuThr: 6.731 ± 0.097
6.426LeuVal: 6.426 ± 0.101
0.834LeuTrp: 0.834 ± 0.031
2.75LeuTyr: 2.75 ± 0.068
0.0LeuXaa: 0.0 ± 0.0
Met
2.363MetAla: 2.363 ± 0.054
0.126MetCys: 0.126 ± 0.01
1.349MetAsp: 1.349 ± 0.037
0.911MetGlu: 0.911 ± 0.035
1.032MetPhe: 1.032 ± 0.04
1.83MetGly: 1.83 ± 0.049
0.475MetHis: 0.475 ± 0.024
2.347MetIle: 2.347 ± 0.06
1.977MetLys: 1.977 ± 0.047
2.262MetLeu: 2.262 ± 0.051
0.827MetMet: 0.827 ± 0.034
1.386MetAsn: 1.386 ± 0.039
1.117MetPro: 1.117 ± 0.036
0.975MetGln: 0.975 ± 0.036
0.939MetArg: 0.939 ± 0.036
1.63MetSer: 1.63 ± 0.042
2.006MetThr: 2.006 ± 0.048
1.857MetVal: 1.857 ± 0.053
0.186MetTrp: 0.186 ± 0.016
0.696MetTyr: 0.696 ± 0.031
0.0MetXaa: 0.0 ± 0.0
Asn
3.715AsnAla: 3.715 ± 0.082
0.218AsnCys: 0.218 ± 0.017
3.112AsnAsp: 3.112 ± 0.066
2.614AsnGlu: 2.614 ± 0.059
2.16AsnPhe: 2.16 ± 0.05
3.915AsnGly: 3.915 ± 0.079
1.57AsnHis: 1.57 ± 0.047
3.102AsnIle: 3.102 ± 0.059
3.039AsnLys: 3.039 ± 0.064
4.694AsnLeu: 4.694 ± 0.078
1.296AsnMet: 1.296 ± 0.042
2.716AsnAsn: 2.716 ± 0.074
2.397AsnPro: 2.397 ± 0.053
3.344AsnGln: 3.344 ± 0.083
2.526AsnArg: 2.526 ± 0.064
3.143AsnSer: 3.143 ± 0.067
2.677AsnThr: 2.677 ± 0.061
3.411AsnVal: 3.411 ± 0.073
0.715AsnTrp: 0.715 ± 0.029
2.082AsnTyr: 2.082 ± 0.051
0.001AsnXaa: 0.001 ± 0.001
Pro
2.757ProAla: 2.757 ± 0.067
0.107ProCys: 0.107 ± 0.011
2.59ProAsp: 2.59 ± 0.069
2.354ProGlu: 2.354 ± 0.07
1.788ProPhe: 1.788 ± 0.049
2.299ProGly: 2.299 ± 0.053
0.728ProHis: 0.728 ± 0.032
2.923ProIle: 2.923 ± 0.056
2.579ProLys: 2.579 ± 0.06
3.243ProLeu: 3.243 ± 0.065
0.84ProMet: 0.84 ± 0.031
2.063ProAsn: 2.063 ± 0.049
0.55ProPro: 0.55 ± 0.03
1.571ProGln: 1.571 ± 0.043
1.193ProArg: 1.193 ± 0.037
2.257ProSer: 2.257 ± 0.051
2.601ProThr: 2.601 ± 0.055
2.798ProVal: 2.798 ± 0.062
0.413ProTrp: 0.413 ± 0.025
1.434ProTyr: 1.434 ± 0.046
0.0ProXaa: 0.0 ± 0.0
Gln
3.713GlnAla: 3.713 ± 0.075
0.115GlnCys: 0.115 ± 0.013
1.996GlnAsp: 1.996 ± 0.06
1.844GlnGlu: 1.844 ± 0.044
1.976GlnPhe: 1.976 ± 0.05
2.206GlnGly: 2.206 ± 0.055
0.971GlnHis: 0.971 ± 0.037
3.682GlnIle: 3.682 ± 0.061
3.2GlnLys: 3.2 ± 0.063
5.179GlnLeu: 5.179 ± 0.092
1.257GlnMet: 1.257 ± 0.042
2.333GlnAsn: 2.333 ± 0.053
1.749GlnPro: 1.749 ± 0.045
2.584GlnGln: 2.584 ± 0.065
2.141GlnArg: 2.141 ± 0.053
2.922GlnSer: 2.922 ± 0.061
3.039GlnThr: 3.039 ± 0.06
3.097GlnVal: 3.097 ± 0.059
0.404GlnTrp: 0.404 ± 0.021
1.676GlnTyr: 1.676 ± 0.049
0.0GlnXaa: 0.0 ± 0.0
Arg
2.482ArgAla: 2.482 ± 0.055
0.167ArgCys: 0.167 ± 0.014
2.137ArgAsp: 2.137 ± 0.048
1.951ArgGlu: 1.951 ± 0.057
1.797ArgPhe: 1.797 ± 0.054
2.361ArgGly: 2.361 ± 0.059
1.066ArgHis: 1.066 ± 0.033
3.096ArgIle: 3.096 ± 0.063
2.731ArgLys: 2.731 ± 0.057
4.039ArgLeu: 4.039 ± 0.07
1.062ArgMet: 1.062 ± 0.033
1.935ArgAsn: 1.935 ± 0.051
1.512ArgPro: 1.512 ± 0.037
2.217ArgGln: 2.217 ± 0.051
2.017ArgArg: 2.017 ± 0.053
2.264ArgSer: 2.264 ± 0.05
2.116ArgThr: 2.116 ± 0.055
2.66ArgVal: 2.66 ± 0.056
0.394ArgTrp: 0.394 ± 0.023
1.687ArgTyr: 1.687 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
4.707SerAla: 4.707 ± 0.09
0.248SerCys: 0.248 ± 0.018
3.927SerAsp: 3.927 ± 0.079
3.01SerGlu: 3.01 ± 0.06
3.042SerPhe: 3.042 ± 0.069
4.997SerGly: 4.997 ± 0.094
1.354SerHis: 1.354 ± 0.04
4.334SerIle: 4.334 ± 0.076
4.275SerLys: 4.275 ± 0.084
6.276SerLeu: 6.276 ± 0.083
1.616SerMet: 1.616 ± 0.038
3.228SerAsn: 3.228 ± 0.06
2.181SerPro: 2.181 ± 0.048
3.095SerGln: 3.095 ± 0.071
2.592SerArg: 2.592 ± 0.046
4.464SerSer: 4.464 ± 0.118
3.878SerThr: 3.878 ± 0.078
4.33SerVal: 4.33 ± 0.075
0.746SerTrp: 0.746 ± 0.033
2.229SerTyr: 2.229 ± 0.048
0.0SerXaa: 0.0 ± 0.0
Thr
4.755ThrAla: 4.755 ± 0.091
0.239ThrCys: 0.239 ± 0.02
3.776ThrAsp: 3.776 ± 0.081
2.573ThrGlu: 2.573 ± 0.062
2.685ThrPhe: 2.685 ± 0.054
4.714ThrGly: 4.714 ± 0.079
1.296ThrHis: 1.296 ± 0.036
4.939ThrIle: 4.939 ± 0.072
4.098ThrLys: 4.098 ± 0.078
5.552ThrLeu: 5.552 ± 0.08
1.369ThrMet: 1.369 ± 0.038
3.461ThrAsn: 3.461 ± 0.081
2.941ThrPro: 2.941 ± 0.075
2.263ThrGln: 2.263 ± 0.059
2.04ThrArg: 2.04 ± 0.054
3.912ThrSer: 3.912 ± 0.08
4.336ThrThr: 4.336 ± 0.097
4.586ThrVal: 4.586 ± 0.072
0.645ThrTrp: 0.645 ± 0.026
2.247ThrTyr: 2.247 ± 0.049
0.002ThrXaa: 0.002 ± 0.002
Val
5.726ValAla: 5.726 ± 0.095
0.383ValCys: 0.383 ± 0.021
4.287ValAsp: 4.287 ± 0.088
3.05ValGlu: 3.05 ± 0.072
2.767ValPhe: 2.767 ± 0.06
4.967ValGly: 4.967 ± 0.078
1.299ValHis: 1.299 ± 0.04
5.456ValIle: 5.456 ± 0.096
4.601ValLys: 4.601 ± 0.076
6.075ValLeu: 6.075 ± 0.092
1.786ValMet: 1.786 ± 0.059
3.746ValAsn: 3.746 ± 0.074
2.697ValPro: 2.697 ± 0.058
2.369ValGln: 2.369 ± 0.053
2.384ValArg: 2.384 ± 0.06
4.916ValSer: 4.916 ± 0.081
4.907ValThr: 4.907 ± 0.086
5.061ValVal: 5.061 ± 0.09
0.61ValTrp: 0.61 ± 0.028
2.319ValTyr: 2.319 ± 0.054
0.0ValXaa: 0.0 ± 0.0
Trp
0.612TrpAla: 0.612 ± 0.024
0.051TrpCys: 0.051 ± 0.008
0.524TrpAsp: 0.524 ± 0.026
0.413TrpGlu: 0.413 ± 0.022
0.519TrpPhe: 0.519 ± 0.03
0.697TrpGly: 0.697 ± 0.024
0.309TrpHis: 0.309 ± 0.018
0.81TrpIle: 0.81 ± 0.035
0.492TrpLys: 0.492 ± 0.024
1.356TrpLeu: 1.356 ± 0.043
0.276TrpMet: 0.276 ± 0.017
0.577TrpAsn: 0.577 ± 0.03
0.32TrpPro: 0.32 ± 0.019
0.688TrpGln: 0.688 ± 0.034
0.486TrpArg: 0.486 ± 0.025
0.673TrpSer: 0.673 ± 0.028
0.564TrpThr: 0.564 ± 0.028
0.726TrpVal: 0.726 ± 0.034
0.157TrpTrp: 0.157 ± 0.013
0.439TrpTyr: 0.439 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.385TyrAla: 2.385 ± 0.055
0.209TyrCys: 0.209 ± 0.015
2.172TyrAsp: 2.172 ± 0.056
1.632TyrGlu: 1.632 ± 0.045
1.858TyrPhe: 1.858 ± 0.049
2.503TyrGly: 2.503 ± 0.063
1.098TyrHis: 1.098 ± 0.041
2.122TyrIle: 2.122 ± 0.052
1.771TyrLys: 1.771 ± 0.048
4.047TyrLeu: 4.047 ± 0.065
0.762TyrMet: 0.762 ± 0.028
1.636TyrAsn: 1.636 ± 0.05
1.462TyrPro: 1.462 ± 0.041
2.413TyrGln: 2.413 ± 0.052
1.853TyrArg: 1.853 ± 0.049
2.292TyrSer: 2.292 ± 0.059
2.007TyrThr: 2.007 ± 0.052
2.287TyrVal: 2.287 ± 0.052
0.476TyrTrp: 0.476 ± 0.021
1.581TyrTyr: 1.581 ± 0.052
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.002XaaAla: 0.002 ± 0.002
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.001XaaLys: 0.001 ± 0.001
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.097XaaXaa: 0.097 ± 0.064
Statistics based on 2962 proteins (886848 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski