SARS coronavirus WH20

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Betacoronavirus; Sarbecovirus; Severe acute respiratory syndrome-related coronavirus

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5DIC1|Q5DIC1_SARS Membrane protein OS=SARS coronavirus WH20 OX=312027 GN=M PE=3 SV=1
MM1 pKa = 7.01FHH3 pKa = 6.99LVDD6 pKa = 4.26FQVTIAEE13 pKa = 3.91ILIIIMRR20 pKa = 11.84TFRR23 pKa = 11.84IAIWNLDD30 pKa = 3.78VIISSIVRR38 pKa = 11.84QLFKK42 pKa = 10.87PLTKK46 pKa = 10.29KK47 pKa = 10.59NYY49 pKa = 10.19SEE51 pKa = 5.43LDD53 pKa = 3.55DD54 pKa = 4.56EE55 pKa = 5.15EE56 pKa = 5.99LMEE59 pKa = 5.81LDD61 pKa = 4.03YY62 pKa = 11.29PP63 pKa = 4.41

Molecular weight: 7.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q5DIC4|Q5DIC4_SARS Uncharacterized protein 1 OS=SARS coronavirus WH20 OX=312027 PE=4 SV=1
MM1 pKa = 7.29MPTTLFAGTHH11 pKa = 4.14ITMTTVYY18 pKa = 9.61HH19 pKa = 5.67ITVSQIQLSLLKK31 pKa = 9.69VTAFQHH37 pKa = 5.32QNSKK41 pKa = 8.78KK42 pKa = 5.45TTKK45 pKa = 10.43LVVILRR51 pKa = 11.84IGTQVLKK58 pKa = 10.45TMSLYY63 pKa = 10.0MAISPKK69 pKa = 9.51FTTSLSLHH77 pKa = 6.65KK78 pKa = 10.58LLQTLVLKK86 pKa = 9.49MLHH89 pKa = 6.16SSSLTSLLKK98 pKa = 7.42THH100 pKa = 7.03RR101 pKa = 11.84MCKK104 pKa = 8.43YY105 pKa = 7.15TQSTALQEE113 pKa = 4.76LLIQQWIQFMMSRR126 pKa = 11.84RR127 pKa = 11.84RR128 pKa = 11.84LLACLCKK135 pKa = 10.12HH136 pKa = 6.19KK137 pKa = 10.69KK138 pKa = 10.15VSTNLCTHH146 pKa = 6.46SFRR149 pKa = 11.84KK150 pKa = 9.4KK151 pKa = 8.19QVRR154 pKa = 3.32

Molecular weight: 17.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

14021

63

7073

1402.1

156.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.203 ± 0.274

3.16 ± 0.322

5.264 ± 0.409

4.793 ± 0.57

4.764 ± 0.354

6.084 ± 0.244

2.061 ± 0.188

5.042 ± 0.345

5.713 ± 0.281

9.693 ± 0.499

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.468 ± 0.191

5.171 ± 0.271

3.994 ± 0.272

3.587 ± 0.539

3.652 ± 0.359

6.747 ± 0.33

7.268 ± 0.392

7.888 ± 0.548

1.084 ± 0.104

4.365 ± 0.206
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski