Amino acid dipepetide frequency for SARS coronavirus WH20

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.348AlaAla: 6.348 ± 0.535
2.282AlaCys: 2.282 ± 0.344
2.996AlaAsp: 2.996 ± 0.343
2.425AlaGlu: 2.425 ± 0.394
2.782AlaPhe: 2.782 ± 0.292
4.494AlaGly: 4.494 ± 0.298
0.927AlaHis: 0.927 ± 0.315
4.28AlaIle: 4.28 ± 0.377
4.137AlaLys: 4.137 ± 1.061
7.347AlaLeu: 7.347 ± 0.655
2.568AlaMet: 2.568 ± 0.309
3.78AlaAsn: 3.78 ± 0.559
2.639AlaPro: 2.639 ± 0.382
2.282AlaGln: 2.282 ± 0.294
2.924AlaArg: 2.924 ± 0.454
4.993AlaSer: 4.993 ± 0.902
4.922AlaThr: 4.922 ± 0.546
4.565AlaVal: 4.565 ± 0.759
1.355AlaTrp: 1.355 ± 0.216
3.994AlaTyr: 3.994 ± 0.425
0.0AlaXaa: 0.0 ± 0.0
Cys
2.568CysAla: 2.568 ± 0.381
1.569CysCys: 1.569 ± 0.205
2.211CysAsp: 2.211 ± 0.404
1.213CysGlu: 1.213 ± 0.282
1.427CysPhe: 1.427 ± 0.418
2.568CysGly: 2.568 ± 0.512
0.571CysHis: 0.571 ± 0.114
1.854CysIle: 1.854 ± 0.488
0.927CysLys: 0.927 ± 0.384
2.853CysLeu: 2.853 ± 0.509
0.642CysMet: 0.642 ± 0.153
1.427CysAsn: 1.427 ± 0.269
0.856CysPro: 0.856 ± 0.157
0.499CysGln: 0.499 ± 0.178
1.07CysArg: 1.07 ± 0.285
1.854CysSer: 1.854 ± 0.434
2.354CysThr: 2.354 ± 0.421
3.138CysVal: 3.138 ± 0.544
0.357CysTrp: 0.357 ± 0.576
1.641CysTyr: 1.641 ± 0.348
0.0CysXaa: 0.0 ± 0.0
Asp
4.779AspAla: 4.779 ± 0.841
1.284AspCys: 1.284 ± 0.261
2.568AspAsp: 2.568 ± 0.419
2.853AspGlu: 2.853 ± 0.263
2.496AspPhe: 2.496 ± 0.535
3.994AspGly: 3.994 ± 0.573
0.856AspHis: 0.856 ± 0.193
2.924AspIle: 2.924 ± 0.512
2.639AspLys: 2.639 ± 0.552
4.779AspLeu: 4.779 ± 0.442
1.213AspMet: 1.213 ± 0.288
3.281AspAsn: 3.281 ± 0.442
1.284AspPro: 1.284 ± 0.357
1.498AspGln: 1.498 ± 0.252
1.355AspArg: 1.355 ± 0.353
3.067AspSer: 3.067 ± 0.351
3.709AspThr: 3.709 ± 0.553
4.208AspVal: 4.208 ± 0.917
0.499AspTrp: 0.499 ± 0.178
3.352AspTyr: 3.352 ± 0.366
0.0AspXaa: 0.0 ± 0.0
Glu
3.352GluAla: 3.352 ± 0.591
1.641GluCys: 1.641 ± 0.318
2.639GluAsp: 2.639 ± 0.391
4.779GluGlu: 4.779 ± 0.884
1.926GluPhe: 1.926 ± 0.521
2.782GluGly: 2.782 ± 0.386
1.498GluHis: 1.498 ± 0.265
2.924GluIle: 2.924 ± 0.364
1.854GluLys: 1.854 ± 0.41
4.708GluLeu: 4.708 ± 0.779
0.927GluMet: 0.927 ± 0.286
1.854GluAsn: 1.854 ± 0.34
2.068GluPro: 2.068 ± 0.276
1.997GluGln: 1.997 ± 0.24
1.284GluArg: 1.284 ± 0.289
2.496GluSer: 2.496 ± 0.228
2.924GluThr: 2.924 ± 0.561
3.852GluVal: 3.852 ± 0.569
0.499GluTrp: 0.499 ± 0.15
1.926GluTyr: 1.926 ± 0.494
0.0GluXaa: 0.0 ± 0.0
Phe
2.782PheAla: 2.782 ± 0.629
1.926PheCys: 1.926 ± 0.43
2.853PheAsp: 2.853 ± 0.555
1.712PheGlu: 1.712 ± 0.422
2.282PhePhe: 2.282 ± 0.173
2.853PheGly: 2.853 ± 0.57
0.642PheHis: 0.642 ± 0.21
2.282PheIle: 2.282 ± 0.232
3.067PheLys: 3.067 ± 0.635
5.35PheLeu: 5.35 ± 1.288
0.999PheMet: 0.999 ± 0.308
2.924PheAsn: 2.924 ± 0.977
1.926PhePro: 1.926 ± 0.285
0.927PheGln: 0.927 ± 0.639
1.498PheArg: 1.498 ± 0.392
3.21PheSer: 3.21 ± 0.572
3.923PheThr: 3.923 ± 0.45
3.638PheVal: 3.638 ± 0.82
0.285PheTrp: 0.285 ± 0.111
2.568PheTyr: 2.568 ± 0.474
0.0PheXaa: 0.0 ± 0.0
Gly
4.636GlyAla: 4.636 ± 0.529
1.569GlyCys: 1.569 ± 0.347
3.78GlyAsp: 3.78 ± 0.442
2.068GlyGlu: 2.068 ± 0.46
3.424GlyPhe: 3.424 ± 0.449
4.066GlyGly: 4.066 ± 0.615
1.498GlyHis: 1.498 ± 0.324
3.852GlyIle: 3.852 ± 0.549
2.924GlyLys: 2.924 ± 0.487
3.424GlyLeu: 3.424 ± 0.53
1.07GlyMet: 1.07 ± 0.28
2.853GlyAsn: 2.853 ± 0.376
2.425GlyPro: 2.425 ± 0.531
2.14GlyGln: 2.14 ± 0.364
1.783GlyArg: 1.783 ± 0.234
3.994GlySer: 3.994 ± 0.32
5.35GlyThr: 5.35 ± 0.974
6.562GlyVal: 6.562 ± 0.533
0.357GlyTrp: 0.357 ± 0.276
3.067GlyTyr: 3.067 ± 0.24
0.0GlyXaa: 0.0 ± 0.0
His
1.427HisAla: 1.427 ± 0.275
0.856HisCys: 0.856 ± 0.33
0.856HisAsp: 0.856 ± 0.333
0.999HisGlu: 0.999 ± 0.18
1.141HisPhe: 1.141 ± 0.445
1.641HisGly: 1.641 ± 0.174
0.571HisHis: 0.571 ± 0.174
1.141HisIle: 1.141 ± 0.456
0.785HisLys: 0.785 ± 0.324
2.282HisLeu: 2.282 ± 0.201
0.428HisMet: 0.428 ± 0.186
0.999HisAsn: 0.999 ± 0.203
0.499HisPro: 0.499 ± 0.178
0.428HisGln: 0.428 ± 0.163
0.214HisArg: 0.214 ± 0.181
1.712HisSer: 1.712 ± 0.425
1.997HisThr: 1.997 ± 0.459
1.569HisVal: 1.569 ± 0.347
0.357HisTrp: 0.357 ± 0.125
0.713HisTyr: 0.713 ± 0.157
0.0HisXaa: 0.0 ± 0.0
Ile
3.566IleAla: 3.566 ± 0.931
1.498IleCys: 1.498 ± 0.277
3.21IleAsp: 3.21 ± 0.287
1.712IleGlu: 1.712 ± 0.3
1.498IlePhe: 1.498 ± 0.265
3.281IleGly: 3.281 ± 0.957
0.499IleHis: 0.499 ± 0.146
2.853IleIle: 2.853 ± 0.985
3.852IleLys: 3.852 ± 0.531
4.351IleLeu: 4.351 ± 0.413
1.712IleMet: 1.712 ± 0.379
2.853IleAsn: 2.853 ± 0.339
1.783IlePro: 1.783 ± 0.445
2.282IleGln: 2.282 ± 0.495
1.569IleArg: 1.569 ± 0.187
3.281IleSer: 3.281 ± 0.607
4.636IleThr: 4.636 ± 0.479
4.565IleVal: 4.565 ± 0.724
0.428IleTrp: 0.428 ± 0.195
0.999IleTyr: 0.999 ± 0.562
0.0IleXaa: 0.0 ± 0.0
Lys
2.853LysAla: 2.853 ± 0.687
1.854LysCys: 1.854 ± 0.272
2.924LysAsp: 2.924 ± 0.496
2.782LysGlu: 2.782 ± 0.348
2.782LysPhe: 2.782 ± 0.498
4.993LysGly: 4.993 ± 0.666
1.854LysHis: 1.854 ± 0.338
2.282LysIle: 2.282 ± 0.407
3.21LysLys: 3.21 ± 1.146
6.348LysLeu: 6.348 ± 0.318
1.569LysMet: 1.569 ± 0.258
2.14LysAsn: 2.14 ± 0.3
3.566LysPro: 3.566 ± 0.328
1.427LysGln: 1.427 ± 0.594
2.496LysArg: 2.496 ± 0.264
4.28LysSer: 4.28 ± 0.623
3.78LysThr: 3.78 ± 0.609
3.138LysVal: 3.138 ± 0.525
0.713LysTrp: 0.713 ± 0.161
2.14LysTyr: 2.14 ± 0.45
0.0LysXaa: 0.0 ± 0.0
Leu
6.419LeuAla: 6.419 ± 0.838
2.924LeuCys: 2.924 ± 0.409
5.492LeuAsp: 5.492 ± 0.926
4.636LeuGlu: 4.636 ± 0.985
3.067LeuPhe: 3.067 ± 0.881
5.136LeuGly: 5.136 ± 0.41
1.712LeuHis: 1.712 ± 0.326
2.924LeuIle: 2.924 ± 0.57
7.061LeuLys: 7.061 ± 0.632
10.271LeuLeu: 10.271 ± 2.289
2.924LeuMet: 2.924 ± 0.318
6.633LeuAsn: 6.633 ± 0.591
4.494LeuPro: 4.494 ± 0.466
4.494LeuGln: 4.494 ± 0.331
4.85LeuArg: 4.85 ± 0.449
7.561LeuSer: 7.561 ± 1.093
5.706LeuThr: 5.706 ± 0.51
5.991LeuVal: 5.991 ± 1.598
1.07LeuTrp: 1.07 ± 0.344
3.566LeuTyr: 3.566 ± 1.077
0.0LeuXaa: 0.0 ± 0.0
Met
1.783MetAla: 1.783 ± 0.653
0.927MetCys: 0.927 ± 0.23
1.712MetAsp: 1.712 ± 0.345
0.927MetGlu: 0.927 ± 0.459
0.927MetPhe: 0.927 ± 0.206
0.927MetGly: 0.927 ± 0.193
0.499MetHis: 0.499 ± 0.165
0.571MetIle: 0.571 ± 0.304
0.927MetLys: 0.927 ± 0.154
3.067MetLeu: 3.067 ± 0.53
0.713MetMet: 0.713 ± 0.335
0.856MetAsn: 0.856 ± 0.188
1.427MetPro: 1.427 ± 0.259
1.284MetGln: 1.284 ± 0.226
0.856MetArg: 0.856 ± 0.392
2.211MetSer: 2.211 ± 0.434
1.427MetThr: 1.427 ± 0.259
1.569MetVal: 1.569 ± 0.386
0.713MetTrp: 0.713 ± 0.38
1.355MetTyr: 1.355 ± 0.155
0.0MetXaa: 0.0 ± 0.0
Asn
4.208AsnAla: 4.208 ± 0.484
1.641AsnCys: 1.641 ± 0.433
1.712AsnAsp: 1.712 ± 0.203
1.854AsnGlu: 1.854 ± 0.306
2.068AsnPhe: 2.068 ± 1.016
4.494AsnGly: 4.494 ± 0.646
1.427AsnHis: 1.427 ± 0.3
2.568AsnIle: 2.568 ± 0.425
2.639AsnLys: 2.639 ± 0.24
4.779AsnLeu: 4.779 ± 0.549
1.427AsnMet: 1.427 ± 0.38
3.424AsnAsn: 3.424 ± 0.358
1.854AsnPro: 1.854 ± 0.565
1.284AsnGln: 1.284 ± 0.419
1.997AsnArg: 1.997 ± 0.462
3.709AsnSer: 3.709 ± 0.817
3.138AsnThr: 3.138 ± 0.442
4.494AsnVal: 4.494 ± 0.449
0.428AsnTrp: 0.428 ± 0.173
2.496AsnTyr: 2.496 ± 0.432
0.0AsnXaa: 0.0 ± 0.0
Pro
2.924ProAla: 2.924 ± 0.335
1.213ProCys: 1.213 ± 0.331
1.712ProAsp: 1.712 ± 0.346
1.569ProGlu: 1.569 ± 0.219
2.068ProPhe: 2.068 ± 0.697
1.854ProGly: 1.854 ± 0.331
0.642ProHis: 0.642 ± 0.185
2.639ProIle: 2.639 ± 0.287
2.924ProLys: 2.924 ± 0.481
4.708ProLeu: 4.708 ± 0.4
0.499ProMet: 0.499 ± 0.195
2.282ProAsn: 2.282 ± 0.233
1.569ProPro: 1.569 ± 0.145
1.355ProGln: 1.355 ± 0.666
1.569ProArg: 1.569 ± 0.39
2.354ProSer: 2.354 ± 0.368
3.067ProThr: 3.067 ± 0.366
3.638ProVal: 3.638 ± 0.546
0.285ProTrp: 0.285 ± 0.086
1.07ProTyr: 1.07 ± 0.141
0.0ProXaa: 0.0 ± 0.0
Gln
2.996GlnAla: 2.996 ± 0.26
1.07GlnCys: 1.07 ± 0.148
1.569GlnAsp: 1.569 ± 0.399
1.854GlnGlu: 1.854 ± 0.253
1.569GlnPhe: 1.569 ± 0.645
1.997GlnGly: 1.997 ± 0.659
0.713GlnHis: 0.713 ± 0.249
1.641GlnIle: 1.641 ± 0.988
1.427GlnLys: 1.427 ± 0.315
3.852GlnLeu: 3.852 ± 0.558
0.999GlnMet: 0.999 ± 0.186
1.355GlnAsn: 1.355 ± 0.31
2.211GlnPro: 2.211 ± 0.292
1.854GlnGln: 1.854 ± 0.381
1.569GlnArg: 1.569 ± 0.326
2.211GlnSer: 2.211 ± 0.246
2.425GlnThr: 2.425 ± 0.336
2.71GlnVal: 2.71 ± 0.54
0.713GlnTrp: 0.713 ± 0.235
1.141GlnTyr: 1.141 ± 0.223
0.0GlnXaa: 0.0 ± 0.0
Arg
3.638ArgAla: 3.638 ± 0.641
1.141ArgCys: 1.141 ± 0.256
1.997ArgAsp: 1.997 ± 0.435
2.425ArgGlu: 2.425 ± 0.51
1.498ArgPhe: 1.498 ± 0.314
2.14ArgGly: 2.14 ± 0.903
1.141ArgHis: 1.141 ± 0.149
1.854ArgIle: 1.854 ± 0.706
1.997ArgLys: 1.997 ± 0.414
2.924ArgLeu: 2.924 ± 0.326
0.642ArgMet: 0.642 ± 0.512
1.712ArgAsn: 1.712 ± 0.509
1.07ArgPro: 1.07 ± 0.255
1.712ArgGln: 1.712 ± 0.456
1.07ArgArg: 1.07 ± 0.741
2.639ArgSer: 2.639 ± 0.374
1.712ArgThr: 1.712 ± 0.511
3.424ArgVal: 3.424 ± 0.431
0.428ArgTrp: 0.428 ± 0.237
1.355ArgTyr: 1.355 ± 0.19
0.0ArgXaa: 0.0 ± 0.0
Ser
5.92SerAla: 5.92 ± 0.982
1.641SerCys: 1.641 ± 0.34
3.852SerAsp: 3.852 ± 0.902
3.709SerGlu: 3.709 ± 0.538
4.137SerPhe: 4.137 ± 0.568
4.137SerGly: 4.137 ± 0.979
1.498SerHis: 1.498 ± 0.396
2.782SerIle: 2.782 ± 0.331
3.495SerLys: 3.495 ± 0.367
6.491SerLeu: 6.491 ± 0.886
1.427SerMet: 1.427 ± 0.27
2.996SerAsn: 2.996 ± 0.609
2.211SerPro: 2.211 ± 0.493
2.354SerGln: 2.354 ± 0.415
1.997SerArg: 1.997 ± 1.136
3.994SerSer: 3.994 ± 0.646
4.922SerThr: 4.922 ± 0.795
5.777SerVal: 5.777 ± 0.608
0.927SerTrp: 0.927 ± 0.134
3.21SerTyr: 3.21 ± 0.454
0.0SerXaa: 0.0 ± 0.0
Thr
3.566ThrAla: 3.566 ± 0.914
2.782ThrCys: 2.782 ± 0.841
2.996ThrAsp: 2.996 ± 0.813
3.852ThrGlu: 3.852 ± 0.425
4.708ThrPhe: 4.708 ± 0.679
4.208ThrGly: 4.208 ± 0.37
1.712ThrHis: 1.712 ± 0.499
4.565ThrIle: 4.565 ± 0.659
3.495ThrLys: 3.495 ± 0.301
6.562ThrLeu: 6.562 ± 0.642
1.641ThrMet: 1.641 ± 0.387
3.281ThrAsn: 3.281 ± 0.259
2.853ThrPro: 2.853 ± 0.317
3.352ThrGln: 3.352 ± 0.7
2.71ThrArg: 2.71 ± 0.379
5.849ThrSer: 5.849 ± 0.842
6.705ThrThr: 6.705 ± 0.839
5.136ThrVal: 5.136 ± 0.569
0.428ThrTrp: 0.428 ± 0.131
2.282ThrTyr: 2.282 ± 0.313
0.0ThrXaa: 0.0 ± 0.0
Val
5.706ValAla: 5.706 ± 0.58
1.926ValCys: 1.926 ± 0.411
4.993ValAsp: 4.993 ± 1.067
4.137ValGlu: 4.137 ± 0.958
4.066ValPhe: 4.066 ± 0.592
3.067ValGly: 3.067 ± 0.506
0.999ValHis: 0.999 ± 0.35
4.137ValIle: 4.137 ± 0.513
5.136ValLys: 5.136 ± 0.569
7.703ValLeu: 7.703 ± 0.697
1.569ValMet: 1.569 ± 0.2
3.281ValAsn: 3.281 ± 0.646
3.138ValPro: 3.138 ± 0.264
2.996ValGln: 2.996 ± 0.392
3.21ValArg: 3.21 ± 0.371
4.636ValSer: 4.636 ± 0.532
6.847ValThr: 6.847 ± 0.851
6.633ValVal: 6.633 ± 0.602
0.571ValTrp: 0.571 ± 0.131
4.137ValTyr: 4.137 ± 0.396
0.0ValXaa: 0.0 ± 0.0
Trp
0.713TrpAla: 0.713 ± 0.214
0.214TrpCys: 0.214 ± 0.069
0.357TrpAsp: 0.357 ± 0.249
0.642TrpGlu: 0.642 ± 0.13
0.999TrpPhe: 0.999 ± 0.208
0.214TrpGly: 0.214 ± 0.121
0.285TrpHis: 0.285 ± 0.164
0.571TrpIle: 0.571 ± 0.256
0.571TrpLys: 0.571 ± 0.147
1.498TrpLeu: 1.498 ± 0.67
0.143TrpMet: 0.143 ± 0.047
1.284TrpAsn: 1.284 ± 0.219
0.428TrpPro: 0.428 ± 0.376
0.285TrpGln: 0.285 ± 0.164
0.214TrpArg: 0.214 ± 0.121
0.856TrpSer: 0.856 ± 0.18
0.428TrpThr: 0.428 ± 0.14
0.785TrpVal: 0.785 ± 0.234
0.071TrpTrp: 0.071 ± 0.05
0.285TrpTyr: 0.285 ± 0.149
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.854TyrAla: 1.854 ± 0.422
1.641TyrCys: 1.641 ± 0.405
2.211TyrAsp: 2.211 ± 0.576
1.783TyrGlu: 1.783 ± 0.281
2.782TyrPhe: 2.782 ± 0.66
1.997TyrGly: 1.997 ± 0.318
1.07TyrHis: 1.07 ± 0.299
1.783TyrIle: 1.783 ± 0.26
4.066TyrLys: 4.066 ± 0.245
3.638TyrLeu: 3.638 ± 0.571
1.498TyrMet: 1.498 ± 0.427
2.496TyrAsn: 2.496 ± 0.276
1.641TyrPro: 1.641 ± 0.413
1.427TyrGln: 1.427 ± 0.67
2.282TyrArg: 2.282 ± 0.26
2.568TyrSer: 2.568 ± 0.788
2.71TyrThr: 2.71 ± 0.436
3.495TyrVal: 3.495 ± 0.457
0.357TyrTrp: 0.357 ± 0.105
2.354TyrTyr: 2.354 ± 0.338
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 10 proteins (14021 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski