Frangipani mosaic virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Tobamovirus

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E2F1P9|E2F1P9_9VIRU Capsid protein OS=Frangipani mosaic virus OX=99585 PE=3 SV=1
MM1 pKa = 7.89SYY3 pKa = 11.05TNISTTNIIYY13 pKa = 10.22LARR16 pKa = 11.84SYY18 pKa = 11.08IPGVDD23 pKa = 4.58FIDD26 pKa = 5.69LIISGLGQSFQTQSARR42 pKa = 11.84DD43 pKa = 3.59EE44 pKa = 4.37FRR46 pKa = 11.84SQLIGSYY53 pKa = 10.02KK54 pKa = 9.83VVVSKK59 pKa = 7.95TVRR62 pKa = 11.84FPEE65 pKa = 3.67NTIYY69 pKa = 10.89LWANNPTIRR78 pKa = 11.84PLLHH82 pKa = 7.05AVFQALDD89 pKa = 3.32TRR91 pKa = 11.84NRR93 pKa = 11.84IIEE96 pKa = 4.13VEE98 pKa = 4.19STNVVNPTSSEE109 pKa = 3.78TRR111 pKa = 11.84EE112 pKa = 3.72ATRR115 pKa = 11.84RR116 pKa = 11.84VDD118 pKa = 3.47DD119 pKa = 3.42ATVAVRR125 pKa = 11.84SQLQLLFDD133 pKa = 4.5ALSGGSGLYY142 pKa = 9.68DD143 pKa = 3.54RR144 pKa = 11.84KK145 pKa = 10.73AFEE148 pKa = 4.72DD149 pKa = 3.62ASGLVWEE156 pKa = 4.79EE157 pKa = 3.51AAAVGTAGTSGTGTTTAA174 pKa = 4.32

Molecular weight: 18.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E2F1P9|E2F1P9_9VIRU Capsid protein OS=Frangipani mosaic virus OX=99585 PE=3 SV=1
MM1 pKa = 7.48ALVEE5 pKa = 4.33LKK7 pKa = 10.38EE8 pKa = 4.08PKK10 pKa = 9.52QLKK13 pKa = 10.49VNDD16 pKa = 3.94FVKK19 pKa = 10.43MSFADD24 pKa = 3.98KK25 pKa = 10.36ILPRR29 pKa = 11.84SLTRR33 pKa = 11.84LRR35 pKa = 11.84TVSISEE41 pKa = 4.26TNVVKK46 pKa = 10.9LSGLGSTVNLNILKK60 pKa = 10.4GVVLNSEE67 pKa = 4.39SKK69 pKa = 9.31YY70 pKa = 7.4VTIRR74 pKa = 11.84GVVISGVWMVPEE86 pKa = 4.63GCGGGATVTLMDD98 pKa = 4.15RR99 pKa = 11.84RR100 pKa = 11.84MKK102 pKa = 10.6GFKK105 pKa = 9.92NGLVAEE111 pKa = 5.18FKK113 pKa = 10.21TRR115 pKa = 11.84ASSRR119 pKa = 11.84DD120 pKa = 3.52FQFKK124 pKa = 10.15FIPNYY129 pKa = 10.04SMCVDD134 pKa = 3.75DD135 pKa = 5.38VKK137 pKa = 10.74RR138 pKa = 11.84APWEE142 pKa = 3.88LFFKK146 pKa = 10.93LVGVPIEE153 pKa = 4.42DD154 pKa = 3.67GYY156 pKa = 11.65YY157 pKa = 9.94PLAIEE162 pKa = 4.13IATLVEE168 pKa = 3.87QSRR171 pKa = 11.84TIINHH176 pKa = 5.58GLRR179 pKa = 11.84ATILKK184 pKa = 10.11RR185 pKa = 11.84CDD187 pKa = 4.45DD188 pKa = 3.75ISDD191 pKa = 4.37LEE193 pKa = 4.44LPSVDD198 pKa = 3.77LDD200 pKa = 3.71EE201 pKa = 6.11SIEE204 pKa = 4.48LISNSNIVSKK214 pKa = 10.9RR215 pKa = 11.84KK216 pKa = 5.88THH218 pKa = 6.42KK219 pKa = 10.1KK220 pKa = 8.83GKK222 pKa = 9.08KK223 pKa = 8.95RR224 pKa = 11.84KK225 pKa = 6.65NTKK228 pKa = 9.05TEE230 pKa = 4.07SEE232 pKa = 4.15SSEE235 pKa = 4.04FARR238 pKa = 11.84VPNFRR243 pKa = 11.84GNDD246 pKa = 3.41LSEE249 pKa = 4.94SGDD252 pKa = 3.6NEE254 pKa = 5.01DD255 pKa = 3.8II256 pKa = 4.75

Molecular weight: 28.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3226

174

1650

806.5

91.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.859 ± 0.329

2.449 ± 0.388

5.952 ± 0.154

6.355 ± 0.125

4.867 ± 0.157

4.898 ± 0.417

2.418 ± 0.393

5.394 ± 0.301

7.347 ± 0.456

9.423 ± 0.135

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.418 ± 0.182

4.278 ± 0.222

3.689 ± 0.134

2.635 ± 0.207

4.681 ± 0.404

8.772 ± 0.167

6.014 ± 0.467

7.812 ± 0.295

1.085 ± 0.053

3.627 ± 0.359
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski