Bacillus selenatarsenatis SF-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Mesobacillus; Mesobacillus selenatarsenatis

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4894 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A8XA56|A0A0A8XA56_9BACI Uncharacterized protein OS=Bacillus selenatarsenatis SF-1 OX=1321606 GN=SAMD00020551_3197 PE=4 SV=1
MM1 pKa = 8.05VEE3 pKa = 4.06VNGSYY8 pKa = 9.71EE9 pKa = 3.99ANIWYY14 pKa = 10.48SFDD17 pKa = 5.47DD18 pKa = 3.68NTKK21 pKa = 9.51TEE23 pKa = 4.18VVTEE27 pKa = 4.01KK28 pKa = 9.87VTYY31 pKa = 10.48CDD33 pKa = 3.76VIKK36 pKa = 10.76LKK38 pKa = 10.89YY39 pKa = 9.72RR40 pKa = 11.84DD41 pKa = 4.12PDD43 pKa = 3.58CMDD46 pKa = 3.6DD47 pKa = 3.82HH48 pKa = 7.17DD49 pKa = 4.61VLVEE53 pKa = 3.93VLQQPNCIEE62 pKa = 4.25AVISPNGNKK71 pKa = 9.86IIVHH75 pKa = 5.12VEE77 pKa = 3.63RR78 pKa = 11.84EE79 pKa = 4.03FLVEE83 pKa = 4.49VIGEE87 pKa = 4.31TKK89 pKa = 10.42VCVVTHH95 pKa = 6.82PGGCDD100 pKa = 3.4CDD102 pKa = 4.35DD103 pKa = 5.07DD104 pKa = 4.22EE105 pKa = 5.0WGHH108 pKa = 7.07GIDD111 pKa = 5.04DD112 pKa = 5.82DD113 pKa = 4.72EE114 pKa = 6.64FEE116 pKa = 5.05DD117 pKa = 4.91LNPDD121 pKa = 3.7FLLGEE126 pKa = 4.31EE127 pKa = 4.38EE128 pKa = 4.16

Molecular weight: 14.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A8WXK6|A0A0A8WXK6_9BACI HTH-type transcriptional regulator LrpC OS=Bacillus selenatarsenatis SF-1 OX=1321606 GN=SAMD00020551_0491 PE=4 SV=1
MM1 pKa = 7.45VLRR4 pKa = 11.84KK5 pKa = 10.0LISAFGATGMMVMLLSVLDD24 pKa = 3.69QSGVGLFFGMYY35 pKa = 9.54ILPIVLIYY43 pKa = 9.32GIPSSIFTDD52 pKa = 4.42FLTRR56 pKa = 11.84RR57 pKa = 11.84TGGLKK62 pKa = 9.81RR63 pKa = 11.84RR64 pKa = 11.84AAAGLLHH71 pKa = 6.4VFLGALFVLIPTLLFDD87 pKa = 5.07AGNGNWLTNLRR98 pKa = 11.84NNGFFFFTAVVSAFIFWRR116 pKa = 11.84FDD118 pKa = 3.21EE119 pKa = 4.48ALKK122 pKa = 11.02SEE124 pKa = 4.06WCGDD128 pKa = 3.63LRR130 pKa = 11.84RR131 pKa = 11.84KK132 pKa = 10.0CLGILKK138 pKa = 10.13RR139 pKa = 11.84IGAMRR144 pKa = 11.84II145 pKa = 3.49

Molecular weight: 16.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4894

0

4894

1317473

37

3035

269.2

30.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.213 ± 0.039

0.633 ± 0.013

5.176 ± 0.029

7.785 ± 0.045

4.678 ± 0.03

7.149 ± 0.035

1.963 ± 0.018

7.622 ± 0.035

7.024 ± 0.035

9.79 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.883 ± 0.017

4.37 ± 0.023

3.632 ± 0.021

3.458 ± 0.023

3.989 ± 0.026

5.999 ± 0.025

5.183 ± 0.026

6.948 ± 0.03

1.031 ± 0.014

3.472 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski