Mycobacteroides abscessus subsp. abscessus
Average proteome isoelectric point is 7.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9486 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1N0E9G0|A0A1N0E9G0_9MYCO Uncharacterized protein OS=Mycobacteroides abscessus subsp. abscessus OX=1185650 GN=SAMEA2259684_05673 PE=4 SV=1
MM1 pKa = 8.13 DD2 pKa = 5.41 PPFGQFDD9 pKa = 3.83 SGADD13 pKa = 3.59 TPEE16 pKa = 5.26 AGADD20 pKa = 3.73 DD21 pKa = 4.83 HH22 pKa = 8.46 DD23 pKa = 5.26 LMIGNTLAEE32 pKa = 4.26 PAGFAVPHH40 pKa = 6.5 TCTSHH45 pKa = 6.26
Molecular weight: 4.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.91
IPC_protein 3.783
Toseland 3.592
ProMoST 3.986
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.808
Rodwell 3.63
Grimsley 3.516
Solomon 3.77
Lehninger 3.732
Nozaki 3.961
DTASelect 4.19
Thurlkill 3.681
EMBOSS 3.808
Sillero 3.923
Patrickios 1.926
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.832
Protein with the highest isoelectric point:
>tr|A0A1M9WNR9|A0A1M9WNR9_9MYCO Cobaltochelatase subunit CobN OS=Mycobacteroides abscessus subsp. abscessus OX=1185650 GN=cobN_1 PE=4 SV=1
MM1 pKa = 7.42 SRR3 pKa = 11.84 RR4 pKa = 11.84 KK5 pKa = 10.13 ASGSALGSGAAGRR18 pKa = 11.84 SRR20 pKa = 11.84 PRR22 pKa = 11.84 ARR24 pKa = 11.84 ASARR28 pKa = 11.84 ASTTIVRR35 pKa = 11.84 PALSSRR41 pKa = 11.84 ARR43 pKa = 11.84 TASSASRR50 pKa = 11.84 WLSTSAA56 pKa = 4.12
Molecular weight: 5.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.522
IPC2_protein 11.228
IPC_protein 12.837
Toseland 12.998
ProMoST 13.495
Dawson 12.998
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.544
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.495
Sillero 12.998
Patrickios 12.281
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.206
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9486
0
9486
1295986
29
1410
136.6
14.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.107 ± 0.039
1.379 ± 0.015
5.243 ± 0.028
4.608 ± 0.03
2.789 ± 0.019
8.464 ± 0.036
2.326 ± 0.02
4.371 ± 0.019
2.338 ± 0.022
8.838 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.627 ± 0.016
2.367 ± 0.019
6.243 ± 0.03
3.122 ± 0.025
7.985 ± 0.036
7.504 ± 0.052
6.308 ± 0.029
8.085 ± 0.033
1.411 ± 0.015
1.852 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here