Paracoccus laeviglucosivorans
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4217 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A521AUU4|A0A521AUU4_9RHOB Uncharacterized membrane protein OS=Paracoccus laeviglucosivorans OX=1197861 GN=SAMN06265221_101374 PE=4 SV=1
MM1 pKa = 7.89 DD2 pKa = 5.72 PSLLVLLLPLGAAAFAGLIVDD23 pKa = 5.08 SNDD26 pKa = 3.08 SDD28 pKa = 4.88 DD29 pKa = 4.59 PVPEE33 pKa = 4.69 PEE35 pKa = 4.9 PEE37 pKa = 4.22 PEE39 pKa = 4.41 PEE41 pKa = 4.41 PEE43 pKa = 4.41 PEE45 pKa = 4.41 PEE47 pKa = 4.41 PEE49 pKa = 4.41 PEE51 pKa = 4.41 PEE53 pKa = 4.41 PEE55 pKa = 4.41 PEE57 pKa = 4.41 PEE59 pKa = 4.41 PEE61 pKa = 4.41 PEE63 pKa = 4.41 PEE65 pKa = 4.37 PEE67 pKa = 4.56 PEE69 pKa = 4.64 PEE71 pKa = 4.26 TITLIGTDD79 pKa = 3.53 GNDD82 pKa = 3.11 RR83 pKa = 11.84 LSNDD87 pKa = 3.58 YY88 pKa = 11.16 YY89 pKa = 11.44 NPTVPYY95 pKa = 9.71 DD96 pKa = 3.41 IRR98 pKa = 11.84 GGAGNDD104 pKa = 3.74 TIIGCDD110 pKa = 3.35 ALASHH115 pKa = 7.29 AGSQQVHH122 pKa = 6.21 HH123 pKa = 6.58 SWHH126 pKa = 6.04 VGEE129 pKa = 5.04 TNDD132 pKa = 4.6 TLDD135 pKa = 4.2 GGAGDD140 pKa = 4.74 DD141 pKa = 5.21 RR142 pKa = 11.84 IFFDD146 pKa = 4.47 LADD149 pKa = 3.69 IVTSGTGADD158 pKa = 3.72 TIGGYY163 pKa = 9.63 VNQGHH168 pKa = 5.34 QAIITDD174 pKa = 4.57 FDD176 pKa = 4.33 PAHH179 pKa = 6.75 DD180 pKa = 3.83 WLRR183 pKa = 11.84 LNFDD187 pKa = 3.9 PDD189 pKa = 3.45 EE190 pKa = 5.49 AGGPDD195 pKa = 3.52 DD196 pKa = 5.25 RR197 pKa = 11.84 FANVSIVEE205 pKa = 4.28 EE206 pKa = 4.31 NGDD209 pKa = 3.74 TVIQLGGIEE218 pKa = 4.07 VTRR221 pKa = 11.84 ILGRR225 pKa = 11.84 TGLNIGYY232 pKa = 7.43 EE233 pKa = 4.47 TNLVRR238 pKa = 11.84 YY239 pKa = 8.9 NYY241 pKa = 10.1 QGVDD245 pKa = 3.45 YY246 pKa = 10.66 VYY248 pKa = 10.66 EE249 pKa = 4.64 ADD251 pKa = 4.1 SDD253 pKa = 4.52 LEE255 pKa = 4.1 AHH257 pKa = 6.09 SEE259 pKa = 3.83 WGGPIHH265 pKa = 7.17 TDD267 pKa = 3.13 LNGNEE272 pKa = 4.31 VARR275 pKa = 11.84 EE276 pKa = 3.88 EE277 pKa = 4.54 LDD279 pKa = 3.73 VVLNSYY285 pKa = 11.14 LSFNTT290 pKa = 3.7
Molecular weight: 31.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.712
IPC2_protein 3.668
IPC_protein 3.681
Toseland 3.465
ProMoST 3.821
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.567
Rodwell 3.503
Grimsley 3.376
Solomon 3.656
Lehninger 3.605
Nozaki 3.77
DTASelect 3.973
Thurlkill 3.503
EMBOSS 3.579
Sillero 3.795
Patrickios 1.227
IPC_peptide 3.643
IPC2_peptide 3.77
IPC2.peptide.svr19 3.729
Protein with the highest isoelectric point:
>tr|A0A521AM48|A0A521AM48_9RHOB Bifunctional protein GlmU OS=Paracoccus laeviglucosivorans OX=1197861 GN=glmU PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNLVRR13 pKa = 11.84 ARR15 pKa = 11.84 RR16 pKa = 11.84 HH17 pKa = 4.37 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.12 GGRR29 pKa = 11.84 RR30 pKa = 11.84 VLNARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.0 GRR40 pKa = 11.84 KK41 pKa = 8.91 SLSAA45 pKa = 3.86
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4217
0
4217
1300680
26
2087
308.4
33.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.843 ± 0.048
0.816 ± 0.012
5.993 ± 0.034
5.296 ± 0.03
3.527 ± 0.024
8.832 ± 0.046
2.077 ± 0.017
5.318 ± 0.028
2.772 ± 0.028
10.284 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.851 ± 0.021
2.525 ± 0.024
5.382 ± 0.031
3.522 ± 0.025
7.187 ± 0.043
4.965 ± 0.022
5.3 ± 0.026
7.025 ± 0.034
1.422 ± 0.016
2.065 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here