Amylibacter sp. SFDW26

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Amylibacter; unclassified Amylibacter

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3070 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I1EFZ7|A0A6I1EFZ7_9RHOB Gamma-glutamyl phosphate reductase OS=Amylibacter sp. SFDW26 OX=2652722 GN=proA PE=3 SV=1
MM1 pKa = 7.71KK2 pKa = 9.19NTLLTTTALALTAGAAYY19 pKa = 10.37ADD21 pKa = 3.72GHH23 pKa = 5.97AVDD26 pKa = 4.09VKK28 pKa = 11.37VGGFYY33 pKa = 9.93NTTVAITSVDD43 pKa = 3.03TGTNFAGTDD52 pKa = 3.68FDD54 pKa = 6.07GIDD57 pKa = 3.34VLSNAEE63 pKa = 4.49IIFKK67 pKa = 10.55PSITLDD73 pKa = 3.09NGIKK77 pKa = 10.16FGAEE81 pKa = 3.49IQLEE85 pKa = 4.24AGSGGGNTAGAATDD99 pKa = 5.61DD100 pKa = 3.55IDD102 pKa = 3.45EE103 pKa = 4.75SYY105 pKa = 11.67VYY107 pKa = 10.32IQGDD111 pKa = 4.19FGQVLIGSEE120 pKa = 3.78NSAGYY125 pKa = 10.56KK126 pKa = 6.96MTVAAPDD133 pKa = 3.39VSPIFAQSSSLTAFVPYY150 pKa = 10.49SGATVGANVFRR161 pKa = 11.84GTLGATYY168 pKa = 10.2IEE170 pKa = 4.23NAGNNDD176 pKa = 3.59ANRR179 pKa = 11.84ITYY182 pKa = 7.67FSPRR186 pKa = 11.84FGGLQLGVSYY196 pKa = 11.05ARR198 pKa = 11.84DD199 pKa = 3.36AGQGNGAVDD208 pKa = 3.9NNAVTTDD215 pKa = 3.9FVDD218 pKa = 3.5IAANYY223 pKa = 8.34GGSFGGVDD231 pKa = 3.21INASARR237 pKa = 11.84YY238 pKa = 7.07GTASAIAGAPDD249 pKa = 3.76PEE251 pKa = 4.41VWGAGLKK258 pKa = 10.6LGFGGFSIGGSYY270 pKa = 10.98AEE272 pKa = 4.16QDD274 pKa = 3.37GTAGGLGDD282 pKa = 3.8GSSYY286 pKa = 11.54DD287 pKa = 3.55VGIGYY292 pKa = 10.77ANGPMSYY299 pKa = 10.57SLTYY303 pKa = 9.94FAGEE307 pKa = 3.87NRR309 pKa = 11.84DD310 pKa = 4.04NEE312 pKa = 4.26GLDD315 pKa = 3.54NVAATADD322 pKa = 3.49DD323 pKa = 4.09GVNRR327 pKa = 11.84EE328 pKa = 4.13SLDD331 pKa = 4.0TIVLAAKK338 pKa = 10.32YY339 pKa = 9.6KK340 pKa = 9.44VNSNFAVSAFVANTQFEE357 pKa = 4.62EE358 pKa = 4.42AGNPADD364 pKa = 4.27DD365 pKa = 4.41VEE367 pKa = 4.53GTVLGVAAQFSFF379 pKa = 3.86

Molecular weight: 38.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I1E7D5|A0A6I1E7D5_9RHOB Octanoyltransferase OS=Amylibacter sp. SFDW26 OX=2652722 GN=lipB PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.26NGRR28 pKa = 11.84IILNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84NQGRR39 pKa = 11.84KK40 pKa = 9.25KK41 pKa = 10.62LSAA44 pKa = 3.91

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3070

0

3070

944714

35

2337

307.7

33.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.079 ± 0.056

0.985 ± 0.014

6.171 ± 0.049

5.971 ± 0.046

4.108 ± 0.032

7.871 ± 0.05

2.036 ± 0.02

6.385 ± 0.036

5.043 ± 0.041

9.493 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.822 ± 0.024

3.735 ± 0.031

4.292 ± 0.026

3.323 ± 0.026

5.096 ± 0.04

5.923 ± 0.034

5.732 ± 0.038

7.013 ± 0.035

1.303 ± 0.019

2.618 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski