Amino acid dipepetide frequency for Amylibacter sp. SFDW26

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.01AlaAla: 11.01 ± 0.145
1.063AlaCys: 1.063 ± 0.034
6.142AlaAsp: 6.142 ± 0.08
6.345AlaGlu: 6.345 ± 0.103
4.0AlaPhe: 4.0 ± 0.07
7.926AlaGly: 7.926 ± 0.106
1.919AlaHis: 1.919 ± 0.046
6.145AlaIle: 6.145 ± 0.089
5.377AlaLys: 5.377 ± 0.097
10.257AlaLeu: 10.257 ± 0.114
3.091AlaMet: 3.091 ± 0.072
3.403AlaAsn: 3.403 ± 0.064
3.959AlaPro: 3.959 ± 0.068
3.834AlaGln: 3.834 ± 0.06
5.013AlaArg: 5.013 ± 0.08
5.502AlaSer: 5.502 ± 0.083
5.077AlaThr: 5.077 ± 0.083
7.026AlaVal: 7.026 ± 0.095
1.066AlaTrp: 1.066 ± 0.034
2.64AlaTyr: 2.64 ± 0.054
0.0AlaXaa: 0.0 ± 0.0
Cys
1.014CysAla: 1.014 ± 0.033
0.134CysCys: 0.134 ± 0.012
0.653CysAsp: 0.653 ± 0.029
0.535CysGlu: 0.535 ± 0.022
0.463CysPhe: 0.463 ± 0.023
0.918CysGly: 0.918 ± 0.033
0.271CysHis: 0.271 ± 0.018
0.611CysIle: 0.611 ± 0.025
0.377CysLys: 0.377 ± 0.021
0.941CysLeu: 0.941 ± 0.033
0.206CysMet: 0.206 ± 0.016
0.305CysAsn: 0.305 ± 0.019
0.473CysPro: 0.473 ± 0.026
0.246CysGln: 0.246 ± 0.016
0.459CysArg: 0.459 ± 0.02
0.618CysSer: 0.618 ± 0.027
0.53CysThr: 0.53 ± 0.023
0.729CysVal: 0.729 ± 0.033
0.125CysTrp: 0.125 ± 0.011
0.239CysTyr: 0.239 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
6.649AspAla: 6.649 ± 0.085
0.547AspCys: 0.547 ± 0.023
3.707AspAsp: 3.707 ± 0.085
3.849AspGlu: 3.849 ± 0.071
2.568AspPhe: 2.568 ± 0.049
5.162AspGly: 5.162 ± 0.109
1.433AspHis: 1.433 ± 0.04
4.194AspIle: 4.194 ± 0.077
2.66AspLys: 2.66 ± 0.058
5.843AspLeu: 5.843 ± 0.081
1.839AspMet: 1.839 ± 0.047
1.869AspAsn: 1.869 ± 0.046
2.805AspPro: 2.805 ± 0.052
2.115AspGln: 2.115 ± 0.051
3.237AspArg: 3.237 ± 0.063
2.202AspSer: 2.202 ± 0.051
3.415AspThr: 3.415 ± 0.077
4.805AspVal: 4.805 ± 0.086
1.084AspTrp: 1.084 ± 0.034
1.732AspTyr: 1.732 ± 0.042
0.0AspXaa: 0.0 ± 0.0
Glu
6.38GluAla: 6.38 ± 0.103
0.46GluCys: 0.46 ± 0.024
3.42GluAsp: 3.42 ± 0.065
3.587GluGlu: 3.587 ± 0.078
2.182GluPhe: 2.182 ± 0.048
4.099GluGly: 4.099 ± 0.084
1.298GluHis: 1.298 ± 0.034
4.055GluIle: 4.055 ± 0.066
3.264GluLys: 3.264 ± 0.063
5.627GluLeu: 5.627 ± 0.077
1.744GluMet: 1.744 ± 0.048
2.712GluAsn: 2.712 ± 0.056
2.221GluPro: 2.221 ± 0.056
2.281GluGln: 2.281 ± 0.051
3.632GluArg: 3.632 ± 0.067
2.278GluSer: 2.278 ± 0.044
4.083GluThr: 4.083 ± 0.074
4.089GluVal: 4.089 ± 0.066
0.776GluTrp: 0.776 ± 0.03
1.523GluTyr: 1.523 ± 0.045
0.0GluXaa: 0.0 ± 0.0
Phe
3.96PheAla: 3.96 ± 0.075
0.548PheCys: 0.548 ± 0.025
2.892PheAsp: 2.892 ± 0.057
2.516PheGlu: 2.516 ± 0.056
1.664PhePhe: 1.664 ± 0.045
3.798PheGly: 3.798 ± 0.068
0.77PheHis: 0.77 ± 0.027
2.508PheIle: 2.508 ± 0.054
1.869PheLys: 1.869 ± 0.045
3.703PheLeu: 3.703 ± 0.074
1.07PheMet: 1.07 ± 0.036
1.52PheAsn: 1.52 ± 0.04
1.558PhePro: 1.558 ± 0.043
1.232PheGln: 1.232 ± 0.032
1.722PheArg: 1.722 ± 0.043
2.752PheSer: 2.752 ± 0.048
2.408PheThr: 2.408 ± 0.048
2.779PheVal: 2.779 ± 0.057
0.577PheTrp: 0.577 ± 0.029
1.231PheTyr: 1.231 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
7.511GlyAla: 7.511 ± 0.09
0.874GlyCys: 0.874 ± 0.033
4.517GlyAsp: 4.517 ± 0.09
4.123GlyGlu: 4.123 ± 0.073
3.762GlyPhe: 3.762 ± 0.062
6.478GlyGly: 6.478 ± 0.107
1.696GlyHis: 1.696 ± 0.044
4.87GlyIle: 4.87 ± 0.083
3.999GlyLys: 3.999 ± 0.069
7.941GlyLeu: 7.941 ± 0.096
2.347GlyMet: 2.347 ± 0.059
2.693GlyAsn: 2.693 ± 0.073
2.725GlyPro: 2.725 ± 0.057
2.894GlyGln: 2.894 ± 0.068
3.946GlyArg: 3.946 ± 0.074
4.36GlySer: 4.36 ± 0.076
4.308GlyThr: 4.308 ± 0.083
6.011GlyVal: 6.011 ± 0.076
1.228GlyTrp: 1.228 ± 0.037
2.423GlyTyr: 2.423 ± 0.055
0.0GlyXaa: 0.0 ± 0.0
His
1.816HisAla: 1.816 ± 0.048
0.255HisCys: 0.255 ± 0.018
1.26HisAsp: 1.26 ± 0.038
1.096HisGlu: 1.096 ± 0.038
0.868HisPhe: 0.868 ± 0.032
1.615HisGly: 1.615 ± 0.044
0.538HisHis: 0.538 ± 0.027
1.347HisIle: 1.347 ± 0.036
0.917HisLys: 0.917 ± 0.035
1.948HisLeu: 1.948 ± 0.052
0.617HisMet: 0.617 ± 0.026
0.717HisAsn: 0.717 ± 0.027
1.187HisPro: 1.187 ± 0.034
0.654HisGln: 0.654 ± 0.026
1.0HisArg: 1.0 ± 0.032
1.138HisSer: 1.138 ± 0.042
0.985HisThr: 0.985 ± 0.031
1.425HisVal: 1.425 ± 0.038
0.341HisTrp: 0.341 ± 0.017
0.639HisTyr: 0.639 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
6.914IleAla: 6.914 ± 0.089
0.749IleCys: 0.749 ± 0.025
4.082IleAsp: 4.082 ± 0.07
4.247IleGlu: 4.247 ± 0.072
2.395IlePhe: 2.395 ± 0.06
5.415IleGly: 5.415 ± 0.095
1.21IleHis: 1.21 ± 0.032
3.881IleIle: 3.881 ± 0.078
2.974IleLys: 2.974 ± 0.062
5.912IleLeu: 5.912 ± 0.098
1.507IleMet: 1.507 ± 0.042
2.272IleAsn: 2.272 ± 0.056
2.746IlePro: 2.746 ± 0.054
1.754IleGln: 1.754 ± 0.044
3.025IleArg: 3.025 ± 0.057
4.249IleSer: 4.249 ± 0.073
3.899IleThr: 3.899 ± 0.064
4.284IleVal: 4.284 ± 0.078
0.788IleTrp: 0.788 ± 0.032
1.543IleTyr: 1.543 ± 0.047
0.0IleXaa: 0.0 ± 0.0
Lys
5.316LysAla: 5.316 ± 0.095
0.361LysCys: 0.361 ± 0.021
3.04LysAsp: 3.04 ± 0.063
2.792LysGlu: 2.792 ± 0.057
1.629LysPhe: 1.629 ± 0.046
3.713LysGly: 3.713 ± 0.072
1.029LysHis: 1.029 ± 0.029
3.106LysIle: 3.106 ± 0.057
2.736LysLys: 2.736 ± 0.072
4.589LysLeu: 4.589 ± 0.077
1.467LysMet: 1.467 ± 0.036
1.978LysAsn: 1.978 ± 0.047
2.486LysPro: 2.486 ± 0.054
1.656LysGln: 1.656 ± 0.04
3.018LysArg: 3.018 ± 0.058
3.228LysSer: 3.228 ± 0.066
3.285LysThr: 3.285 ± 0.06
3.139LysVal: 3.139 ± 0.064
0.625LysTrp: 0.625 ± 0.027
1.24LysTyr: 1.24 ± 0.04
0.0LysXaa: 0.0 ± 0.0
Leu
9.496LeuAla: 9.496 ± 0.125
0.991LeuCys: 0.991 ± 0.034
5.818LeuAsp: 5.818 ± 0.084
5.64LeuGlu: 5.64 ± 0.087
3.733LeuPhe: 3.733 ± 0.076
7.519LeuGly: 7.519 ± 0.104
1.695LeuHis: 1.695 ± 0.05
6.038LeuIle: 6.038 ± 0.092
5.017LeuLys: 5.017 ± 0.081
8.191LeuLeu: 8.191 ± 0.116
2.561LeuMet: 2.561 ± 0.057
3.747LeuAsn: 3.747 ± 0.07
4.461LeuPro: 4.461 ± 0.068
2.984LeuGln: 2.984 ± 0.052
4.979LeuArg: 4.979 ± 0.082
7.165LeuSer: 7.165 ± 0.093
5.587LeuThr: 5.587 ± 0.077
5.971LeuVal: 5.971 ± 0.082
1.116LeuTrp: 1.116 ± 0.035
2.224LeuTyr: 2.224 ± 0.053
0.0LeuXaa: 0.0 ± 0.0
Met
2.959MetAla: 2.959 ± 0.06
0.256MetCys: 0.256 ± 0.015
1.571MetAsp: 1.571 ± 0.043
1.489MetGlu: 1.489 ± 0.045
0.971MetPhe: 0.971 ± 0.039
2.153MetGly: 2.153 ± 0.05
0.49MetHis: 0.49 ± 0.022
1.829MetIle: 1.829 ± 0.046
1.443MetLys: 1.443 ± 0.035
2.582MetLeu: 2.582 ± 0.066
0.872MetMet: 0.872 ± 0.032
1.105MetAsn: 1.105 ± 0.034
1.383MetPro: 1.383 ± 0.039
1.012MetGln: 1.012 ± 0.027
1.643MetArg: 1.643 ± 0.045
1.93MetSer: 1.93 ± 0.044
1.986MetThr: 1.986 ± 0.054
1.82MetVal: 1.82 ± 0.055
0.267MetTrp: 0.267 ± 0.02
0.464MetTyr: 0.464 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
3.904AsnAla: 3.904 ± 0.069
0.356AsnCys: 0.356 ± 0.018
2.273AsnAsp: 2.273 ± 0.064
1.891AsnGlu: 1.891 ± 0.044
1.481AsnPhe: 1.481 ± 0.041
3.325AsnGly: 3.325 ± 0.072
0.798AsnHis: 0.798 ± 0.031
2.531AsnIle: 2.531 ± 0.055
1.663AsnLys: 1.663 ± 0.04
3.443AsnLeu: 3.443 ± 0.061
0.934AsnMet: 0.934 ± 0.033
1.425AsnAsn: 1.425 ± 0.043
2.045AsnPro: 2.045 ± 0.039
1.144AsnGln: 1.144 ± 0.037
1.901AsnArg: 1.901 ± 0.047
1.932AsnSer: 1.932 ± 0.051
2.181AsnThr: 2.181 ± 0.046
2.573AsnVal: 2.573 ± 0.049
0.558AsnTrp: 0.558 ± 0.021
0.991AsnTyr: 0.991 ± 0.037
0.0AsnXaa: 0.0 ± 0.0
Pro
3.67ProAla: 3.67 ± 0.078
0.33ProCys: 0.33 ± 0.019
3.088ProAsp: 3.088 ± 0.059
3.531ProGlu: 3.531 ± 0.066
1.917ProPhe: 1.917 ± 0.042
2.323ProGly: 2.323 ± 0.059
0.912ProHis: 0.912 ± 0.032
2.806ProIle: 2.806 ± 0.057
2.551ProLys: 2.551 ± 0.057
3.695ProLeu: 3.695 ± 0.061
1.233ProMet: 1.233 ± 0.039
2.008ProAsn: 2.008 ± 0.045
1.377ProPro: 1.377 ± 0.045
1.358ProGln: 1.358 ± 0.04
1.817ProArg: 1.817 ± 0.047
2.642ProSer: 2.642 ± 0.056
2.476ProThr: 2.476 ± 0.055
3.436ProVal: 3.436 ± 0.072
0.561ProTrp: 0.561 ± 0.025
1.191ProTyr: 1.191 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
3.463GlnAla: 3.463 ± 0.06
0.246GlnCys: 0.246 ± 0.016
1.843GlnAsp: 1.843 ± 0.048
1.9GlnGlu: 1.9 ± 0.044
1.351GlnPhe: 1.351 ± 0.037
2.249GlnGly: 2.249 ± 0.048
0.652GlnHis: 0.652 ± 0.026
2.257GlnIle: 2.257 ± 0.048
1.899GlnLys: 1.899 ± 0.048
2.898GlnLeu: 2.898 ± 0.057
1.12GlnMet: 1.12 ± 0.03
1.486GlnAsn: 1.486 ± 0.044
1.355GlnPro: 1.355 ± 0.036
1.136GlnGln: 1.136 ± 0.039
1.716GlnArg: 1.716 ± 0.046
2.276GlnSer: 2.276 ± 0.05
2.05GlnThr: 2.05 ± 0.048
2.129GlnVal: 2.129 ± 0.048
0.405GlnTrp: 0.405 ± 0.02
0.798GlnTyr: 0.798 ± 0.029
0.0GlnXaa: 0.0 ± 0.0
Arg
4.653ArgAla: 4.653 ± 0.079
0.431ArgCys: 0.431 ± 0.023
3.261ArgAsp: 3.261 ± 0.06
3.035ArgGlu: 3.035 ± 0.061
2.154ArgPhe: 2.154 ± 0.051
3.304ArgGly: 3.304 ± 0.06
1.067ArgHis: 1.067 ± 0.038
3.392ArgIle: 3.392 ± 0.067
2.786ArgLys: 2.786 ± 0.055
5.305ArgLeu: 5.305 ± 0.086
1.567ArgMet: 1.567 ± 0.04
1.942ArgAsn: 1.942 ± 0.043
2.213ArgPro: 2.213 ± 0.055
1.705ArgGln: 1.705 ± 0.043
2.959ArgArg: 2.959 ± 0.059
3.043ArgSer: 3.043 ± 0.058
2.443ArgThr: 2.443 ± 0.052
3.641ArgVal: 3.641 ± 0.067
0.679ArgTrp: 0.679 ± 0.026
1.376ArgTyr: 1.376 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
5.554SerAla: 5.554 ± 0.078
0.574SerCys: 0.574 ± 0.026
3.879SerAsp: 3.879 ± 0.08
3.437SerGlu: 3.437 ± 0.065
2.786SerPhe: 2.786 ± 0.052
5.431SerGly: 5.431 ± 0.079
1.106SerHis: 1.106 ± 0.037
3.739SerIle: 3.739 ± 0.079
3.017SerLys: 3.017 ± 0.059
5.595SerLeu: 5.595 ± 0.081
1.596SerMet: 1.596 ± 0.04
2.262SerAsn: 2.262 ± 0.055
2.379SerPro: 2.379 ± 0.046
1.949SerGln: 1.949 ± 0.04
2.768SerArg: 2.768 ± 0.054
3.38SerSer: 3.38 ± 0.064
3.053SerThr: 3.053 ± 0.059
4.303SerVal: 4.303 ± 0.065
0.71SerTrp: 0.71 ± 0.026
1.717SerTyr: 1.717 ± 0.046
0.0SerXaa: 0.0 ± 0.0
Thr
5.718ThrAla: 5.718 ± 0.103
0.542ThrCys: 0.542 ± 0.026
3.387ThrAsp: 3.387 ± 0.065
3.107ThrGlu: 3.107 ± 0.064
2.272ThrPhe: 2.272 ± 0.053
4.857ThrGly: 4.857 ± 0.073
1.243ThrHis: 1.243 ± 0.035
3.589ThrIle: 3.589 ± 0.065
2.717ThrLys: 2.717 ± 0.054
5.876ThrLeu: 5.876 ± 0.089
1.363ThrMet: 1.363 ± 0.04
1.959ThrAsn: 1.959 ± 0.05
3.079ThrPro: 3.079 ± 0.058
1.874ThrGln: 1.874 ± 0.046
2.626ThrArg: 2.626 ± 0.055
3.381ThrSer: 3.381 ± 0.055
3.239ThrThr: 3.239 ± 0.062
4.284ThrVal: 4.284 ± 0.076
0.681ThrTrp: 0.681 ± 0.029
1.527ThrTyr: 1.527 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
7.068ValAla: 7.068 ± 0.086
0.71ValCys: 0.71 ± 0.029
4.236ValAsp: 4.236 ± 0.074
4.372ValGlu: 4.372 ± 0.07
3.106ValPhe: 3.106 ± 0.052
5.238ValGly: 5.238 ± 0.078
1.294ValHis: 1.294 ± 0.038
4.704ValIle: 4.704 ± 0.091
3.289ValLys: 3.289 ± 0.06
6.765ValLeu: 6.765 ± 0.094
2.056ValMet: 2.056 ± 0.046
2.54ValAsn: 2.54 ± 0.061
2.859ValPro: 2.859 ± 0.049
2.133ValGln: 2.133 ± 0.045
3.231ValArg: 3.231 ± 0.071
4.681ValSer: 4.681 ± 0.073
4.239ValThr: 4.239 ± 0.077
5.147ValVal: 5.147 ± 0.075
0.808ValTrp: 0.808 ± 0.03
1.653ValTyr: 1.653 ± 0.05
0.0ValXaa: 0.0 ± 0.0
Trp
1.178TrpAla: 1.178 ± 0.035
0.127TrpCys: 0.127 ± 0.012
0.764TrpAsp: 0.764 ± 0.029
0.635TrpGlu: 0.635 ± 0.025
0.603TrpPhe: 0.603 ± 0.025
0.981TrpGly: 0.981 ± 0.036
0.334TrpHis: 0.334 ± 0.02
0.748TrpIle: 0.748 ± 0.034
0.629TrpLys: 0.629 ± 0.026
1.403TrpLeu: 1.403 ± 0.045
0.423TrpMet: 0.423 ± 0.022
0.473TrpAsn: 0.473 ± 0.024
0.573TrpPro: 0.573 ± 0.025
0.466TrpGln: 0.466 ± 0.019
0.83TrpArg: 0.83 ± 0.032
0.813TrpSer: 0.813 ± 0.031
0.661TrpThr: 0.661 ± 0.029
0.888TrpVal: 0.888 ± 0.031
0.21TrpTrp: 0.21 ± 0.016
0.293TrpTyr: 0.293 ± 0.019
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.562TyrAla: 2.562 ± 0.059
0.292TyrCys: 0.292 ± 0.017
1.882TyrAsp: 1.882 ± 0.053
1.586TyrGlu: 1.586 ± 0.036
1.173TyrPhe: 1.173 ± 0.035
2.202TyrGly: 2.202 ± 0.046
0.61TyrHis: 0.61 ± 0.028
1.497TyrIle: 1.497 ± 0.041
1.247TyrLys: 1.247 ± 0.036
2.418TyrLeu: 2.418 ± 0.058
0.609TyrMet: 0.609 ± 0.026
0.929TyrAsn: 0.929 ± 0.03
1.041TyrPro: 1.041 ± 0.035
0.803TyrGln: 0.803 ± 0.033
1.433TyrArg: 1.433 ± 0.036
1.665TyrSer: 1.665 ± 0.044
1.417TyrThr: 1.417 ± 0.041
1.649TyrVal: 1.649 ± 0.04
0.431TyrTrp: 0.431 ± 0.021
0.737TyrTyr: 0.737 ± 0.031
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3070 proteins (944714 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski