Streptomyces taklimakanensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5063 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G2B5V7|A0A6G2B5V7_9ACTN PIG-L family deacetylase OS=Streptomyces taklimakanensis OX=2569853 GN=F0L17_00520 PE=4 SV=1
MM1 pKa = 7.09KK2 pKa = 10.3RR3 pKa = 11.84RR4 pKa = 11.84WARR7 pKa = 11.84GLVAGAGVLALTGTSTLLMAAPAGAAIGNDD37 pKa = 3.24SNANAMASALAGPGADD53 pKa = 3.08VLGAGFEE60 pKa = 4.41SLAGAVANGVGDD72 pKa = 3.97SPLSNFPTNGSTFTILTSGDD92 pKa = 3.34AASADD97 pKa = 3.8DD98 pKa = 4.76PNTEE102 pKa = 4.15GSKK105 pKa = 7.7TTDD108 pKa = 3.26LAGGNVRR115 pKa = 11.84GNTDD119 pKa = 3.43FDD121 pKa = 4.26VSVFTVDD128 pKa = 3.51LQVPEE133 pKa = 4.3TANCLSFDD141 pKa = 3.57FQFYY145 pKa = 10.37SEE147 pKa = 5.59EE148 pKa = 4.0YY149 pKa = 9.03PEE151 pKa = 4.38WVGSAFNDD159 pKa = 3.43AFIAEE164 pKa = 5.07LDD166 pKa = 3.73NSDD169 pKa = 3.09WTTSGSEE176 pKa = 3.56INAPNNFAFDD186 pKa = 3.74ADD188 pKa = 3.77NDD190 pKa = 4.23VVSVNAVGLGGFSAEE205 pKa = 4.07NAAGTTYY212 pKa = 11.12DD213 pKa = 3.39GATPLLSAAKK223 pKa = 9.38QVTPGTHH230 pKa = 5.21TLYY233 pKa = 11.11LSIFDD238 pKa = 3.53QGDD241 pKa = 3.8RR242 pKa = 11.84ILDD245 pKa = 3.48SATFVDD251 pKa = 3.88NLRR254 pKa = 11.84VGFVPDD260 pKa = 5.02PEE262 pKa = 4.58TQCKK266 pKa = 10.24SGAQPKK272 pKa = 9.78PYY274 pKa = 10.07QLDD277 pKa = 3.7LTPVEE282 pKa = 4.5TTLDD286 pKa = 3.33TGTDD290 pKa = 3.42HH291 pKa = 6.99TVTATLTEE299 pKa = 4.38IGAEE303 pKa = 4.07PPAVSGASILFQASGAHH320 pKa = 5.98AVQGSDD326 pKa = 3.08TTDD329 pKa = 2.99TAGQATFTYY338 pKa = 9.39TGTTVGVDD346 pKa = 4.0SISACYY352 pKa = 10.19DD353 pKa = 2.74VDD355 pKa = 4.85GNGTCDD361 pKa = 3.14AGEE364 pKa = 4.31PFASAKK370 pKa = 9.29ATWKK374 pKa = 8.07NTPPVNDD381 pKa = 4.0PGGPYY386 pKa = 10.2SGAEE390 pKa = 4.14GSAVALDD397 pKa = 3.82ATASDD402 pKa = 4.22VNGDD406 pKa = 4.0PLTHH410 pKa = 6.55KK411 pKa = 8.22WTYY414 pKa = 10.33EE415 pKa = 3.85PVSGVDD421 pKa = 3.28TGAVCSFEE429 pKa = 5.45DD430 pKa = 4.86DD431 pKa = 3.88SALEE435 pKa = 3.95TAITCTDD442 pKa = 3.47DD443 pKa = 3.54GEE445 pKa = 4.43YY446 pKa = 10.82RR447 pKa = 11.84LTLTTDD453 pKa = 3.54DD454 pKa = 4.25GVNAPVSNTADD465 pKa = 3.43LTVTNVAPAIGEE477 pKa = 3.97VSTPVDD483 pKa = 3.67PVAVGTPLSLEE494 pKa = 4.1AGYY497 pKa = 8.99TDD499 pKa = 4.3PGANDD504 pKa = 3.26THH506 pKa = 6.33TASIDD511 pKa = 3.3WGDD514 pKa = 3.53GSMSEE519 pKa = 4.08PTAAAGQVDD528 pKa = 4.0ASHH531 pKa = 7.32TYY533 pKa = 8.19TAAGIYY539 pKa = 7.27TICLTVTDD547 pKa = 5.21DD548 pKa = 6.07DD549 pKa = 4.43GASDD553 pKa = 4.18EE554 pKa = 4.6KK555 pKa = 11.08CSPNYY560 pKa = 10.05VVVYY564 pKa = 9.98DD565 pKa = 4.69PSAGFVTGGGWIDD578 pKa = 3.75VPEE581 pKa = 4.53GSYY584 pKa = 10.43PADD587 pKa = 3.9PSASGPGRR595 pKa = 11.84FGFVSKK601 pKa = 9.63YY602 pKa = 8.8QKK604 pKa = 10.81GATAPKK610 pKa = 9.95GQTEE614 pKa = 4.45FQFKK618 pKa = 9.47TGSLNFHH625 pKa = 6.38SMSYY629 pKa = 10.8DD630 pKa = 2.94WLVVSGNKK638 pKa = 9.81AIYY641 pKa = 10.01KK642 pKa = 8.43GTGTVNGEE650 pKa = 3.92DD651 pKa = 3.86GYY653 pKa = 11.5GFLVSVVDD661 pKa = 3.97ADD663 pKa = 4.26KK664 pKa = 11.58SGGDD668 pKa = 3.07DD669 pKa = 3.46TYY671 pKa = 11.14RR672 pKa = 11.84IKK674 pKa = 10.96VWDD677 pKa = 4.27ADD679 pKa = 3.38SDD681 pKa = 4.33TVVFDD686 pKa = 4.2NQPGAADD693 pKa = 3.61DD694 pKa = 4.08TAATTAISHH703 pKa = 6.19GSIVIHH709 pKa = 6.2SS710 pKa = 3.78

Molecular weight: 72.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G2BDV9|A0A6G2BDV9_9ACTN Uncharacterized protein OS=Streptomyces taklimakanensis OX=2569853 GN=F0L17_14605 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIASRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.34GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5063

0

5063

1706788

32

6685

337.1

36.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.314 ± 0.057

0.8 ± 0.01

6.121 ± 0.029

6.347 ± 0.032

2.581 ± 0.016

9.911 ± 0.035

2.356 ± 0.016

2.775 ± 0.026

1.764 ± 0.03

10.049 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.63 ± 0.014

1.639 ± 0.019

6.43 ± 0.036

2.232 ± 0.024

8.982 ± 0.042

4.761 ± 0.027

6.067 ± 0.026

8.791 ± 0.029

1.484 ± 0.015

1.966 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski