Paeniglutamicibacter gangotriensis Lz1y
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4017 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M7NA47|M7NA47_9MICC Putative hydrolase OS=Paeniglutamicibacter gangotriensis Lz1y OX=1276920 GN=ADIAG_02089 PE=4 SV=1
MM1 pKa = 7.3 SKK3 pKa = 10.28 VLASLRR9 pKa = 11.84 YY10 pKa = 8.44 SAEE13 pKa = 4.12 HH14 pKa = 6.11 EE15 pKa = 4.33 WVDD18 pKa = 3.21 ASTPTKK24 pKa = 10.81 VGITQVAADD33 pKa = 3.75 ALGDD37 pKa = 3.73 VVYY40 pKa = 11.27 VDD42 pKa = 4.35 LPEE45 pKa = 5.01 VGDD48 pKa = 4.91 AITAGEE54 pKa = 4.25 TCGEE58 pKa = 4.21 VEE60 pKa = 4.36 STKK63 pKa = 10.75 SVSDD67 pKa = 3.74 LYY69 pKa = 11.56 APVTGTILEE78 pKa = 4.33 INQEE82 pKa = 4.5 AIDD85 pKa = 4.24 NPAILNEE92 pKa = 4.18 DD93 pKa = 3.85 PYY95 pKa = 11.17 GAGWLFTVEE104 pKa = 4.07 VTAEE108 pKa = 4.3 GPLLSAADD116 pKa = 3.76 YY117 pKa = 11.24 ASANGGDD124 pKa = 3.8 VAA126 pKa = 5.53
Molecular weight: 13.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.758
IPC2_protein 3.681
IPC_protein 3.617
Toseland 3.427
ProMoST 3.795
Dawson 3.617
Bjellqvist 3.77
Wikipedia 3.541
Rodwell 3.465
Grimsley 3.35
Solomon 3.592
Lehninger 3.541
Nozaki 3.745
DTASelect 3.91
Thurlkill 3.49
EMBOSS 3.554
Sillero 3.745
Patrickios 0.769
IPC_peptide 3.592
IPC2_peptide 3.719
IPC2.peptide.svr19 3.705
Protein with the highest isoelectric point:
>tr|M7NL12|M7NL12_9MICC Phosphatidate cytidylyltransferase OS=Paeniglutamicibacter gangotriensis Lz1y OX=1276920 GN=ADIAG_01223 PE=3 SV=1
MM1 pKa = 7.05 VPVAAAHH8 pKa = 5.35 VRR10 pKa = 11.84 AVPARR15 pKa = 11.84 VARR18 pKa = 11.84 VLRR21 pKa = 11.84 QVPVVPVPQQAPAVPARR38 pKa = 11.84 QPVQADD44 pKa = 3.54 PVRR47 pKa = 11.84 AHH49 pKa = 7.61 RR50 pKa = 11.84 VRR52 pKa = 11.84 QVLRR56 pKa = 11.84 VQARR60 pKa = 11.84 VPRR63 pKa = 11.84 VALAQLPAA71 pKa = 5.23
Molecular weight: 7.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.407
IPC2_protein 10.877
IPC_protein 12.501
Toseland 12.647
ProMoST 13.159
Dawson 12.647
Bjellqvist 12.647
Wikipedia 13.13
Rodwell 12.149
Grimsley 12.691
Solomon 13.159
Lehninger 13.056
Nozaki 12.647
DTASelect 12.647
Thurlkill 12.647
EMBOSS 13.159
Sillero 12.647
Patrickios 11.901
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.162
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4017
0
4017
1241062
29
2104
309.0
33.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.636 ± 0.05
0.659 ± 0.01
5.365 ± 0.032
5.891 ± 0.034
3.263 ± 0.023
8.772 ± 0.035
2.224 ± 0.017
4.838 ± 0.028
2.997 ± 0.029
10.416 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.307 ± 0.018
2.614 ± 0.021
5.215 ± 0.029
3.055 ± 0.019
6.26 ± 0.039
6.105 ± 0.027
6.061 ± 0.024
7.863 ± 0.03
1.418 ± 0.014
2.04 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here