Ateles paniscus polyomavirus 1
Average proteome isoelectric point is 5.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K7QK07|K7QK07_9POLY Small T antigen OS=Ateles paniscus polyomavirus 1 OX=1236391 PE=4 SV=1
MM1 pKa = 7.46 GGVLSLFFDD10 pKa = 3.5 IAEE13 pKa = 4.41 LASALSTEE21 pKa = 4.34 TGISVGAILAGEE33 pKa = 4.09 AAADD37 pKa = 3.55 IEE39 pKa = 4.31 ASVMGLMTIEE49 pKa = 4.18 GLGAADD55 pKa = 4.18 ALAAMGLTMEE65 pKa = 5.06 NFSMMYY71 pKa = 9.88 ALPGMLSEE79 pKa = 4.54 AVGIGTLFQTISGASSLVAVGIKK102 pKa = 9.75 YY103 pKa = 10.44 SRR105 pKa = 11.84 EE106 pKa = 3.62 EE107 pKa = 3.66 VSIVNKK113 pKa = 10.31 NISDD117 pKa = 3.67 MALQLWRR124 pKa = 11.84 PQDD127 pKa = 3.56 YY128 pKa = 11.22 YY129 pKa = 11.46 DD130 pKa = 3.73 ILFPGVNHH138 pKa = 6.51 FAHH141 pKa = 6.32 YY142 pKa = 10.85 LNVIEE147 pKa = 5.31 GWASSLVHH155 pKa = 5.38 TVSRR159 pKa = 11.84 AIWDD163 pKa = 3.77 SIMRR167 pKa = 11.84 DD168 pKa = 3.06 ARR170 pKa = 11.84 HH171 pKa = 5.53 QIGYY175 pKa = 10.39 ASRR178 pKa = 11.84 EE179 pKa = 3.96 LVVRR183 pKa = 11.84 GTTSFQDD190 pKa = 3.21 VMAKK194 pKa = 10.3 VIEE197 pKa = 4.04 TSRR200 pKa = 11.84 WVLTSGPSHH209 pKa = 7.14 IYY211 pKa = 10.29 SSLSEE216 pKa = 4.43 YY217 pKa = 10.35 YY218 pKa = 10.14 RR219 pKa = 11.84 QLPGLNPAQIRR230 pKa = 11.84 DD231 pKa = 3.44 VYY233 pKa = 10.69 RR234 pKa = 11.84 RR235 pKa = 11.84 LGEE238 pKa = 4.11 PVPSRR243 pKa = 11.84 YY244 pKa = 8.92 QLEE247 pKa = 4.18 VEE249 pKa = 4.17 ARR251 pKa = 11.84 EE252 pKa = 4.02 EE253 pKa = 3.98 SAEE256 pKa = 4.14 VIEE259 pKa = 5.07 TYY261 pKa = 10.25 SAPGGAHH268 pKa = 6.07 QRR270 pKa = 11.84 VCPDD274 pKa = 2.37 WMLPLILGLYY284 pKa = 10.41 GDD286 pKa = 4.05 VTPTFATYY294 pKa = 9.37 IHH296 pKa = 5.77 QVEE299 pKa = 4.4 KK300 pKa = 10.72 EE301 pKa = 3.99 DD302 pKa = 4.53 DD303 pKa = 3.21 KK304 pKa = 11.77 GRR306 pKa = 11.84 PSKK309 pKa = 10.45 RR310 pKa = 11.84 RR311 pKa = 11.84 RR312 pKa = 11.84 LL313 pKa = 3.55
Molecular weight: 34.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.002
IPC2_protein 5.08
IPC_protein 5.016
Toseland 4.978
ProMoST 5.156
Dawson 5.003
Bjellqvist 5.13
Wikipedia 4.863
Rodwell 4.927
Grimsley 4.914
Solomon 5.003
Lehninger 4.965
Nozaki 5.13
DTASelect 5.258
Thurlkill 4.978
EMBOSS 4.914
Sillero 5.207
Patrickios 3.821
IPC_peptide 5.016
IPC2_peptide 5.207
IPC2.peptide.svr19 5.158
Protein with the highest isoelectric point:
>tr|K7QLH4|K7QLH4_9POLY Capsid protein VP1 OS=Ateles paniscus polyomavirus 1 OX=1236391 PE=3 SV=1
MM1 pKa = 7.71 ASKK4 pKa = 10.36 KK5 pKa = 10.09 CPRR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 8.22 TPSCPRR16 pKa = 11.84 KK17 pKa = 9.06 PATCPQTTCPVPRR30 pKa = 11.84 AVPRR34 pKa = 11.84 LLIKK38 pKa = 10.88 GGVEE42 pKa = 3.73 VLAVKK47 pKa = 9.34 TGPDD51 pKa = 3.81 SITQIEE57 pKa = 4.95 AYY59 pKa = 10.36 LNPRR63 pKa = 11.84 MGKK66 pKa = 10.07 AGNDD70 pKa = 3.43 DD71 pKa = 3.54 QYY73 pKa = 11.53 GYY75 pKa = 10.19 SASITVAASLDD86 pKa = 3.31 ADD88 pKa = 3.73 NPKK91 pKa = 10.27 KK92 pKa = 10.73 EE93 pKa = 4.17 EE94 pKa = 4.07 LPTYY98 pKa = 8.96 STAKK102 pKa = 10.17 ISLPLLNEE110 pKa = 4.58 DD111 pKa = 4.2 LTCGTLLMWEE121 pKa = 4.14 AVSVKK126 pKa = 9.88 TEE128 pKa = 3.84 VVGISSLVNVHH139 pKa = 6.17 IPSKK143 pKa = 10.69 RR144 pKa = 11.84 MYY146 pKa = 8.85 NDD148 pKa = 2.99 KK149 pKa = 11.3 GIGFPIEE156 pKa = 3.8 GMNFHH161 pKa = 6.75 MFAVGGEE168 pKa = 4.05 PLEE171 pKa = 4.35 LQAVTSNYY179 pKa = 7.35 KK180 pKa = 9.37 TNYY183 pKa = 8.66 PDD185 pKa = 3.58 GTVKK189 pKa = 10.68 PPVKK193 pKa = 10.57 AKK195 pKa = 8.27 TQQVLDD201 pKa = 3.95 PQYY204 pKa = 10.88 KK205 pKa = 10.44 AKK207 pKa = 10.46 LLKK210 pKa = 10.86 DD211 pKa = 3.17 GAYY214 pKa = 9.64 PIEE217 pKa = 4.66 LWAPDD222 pKa = 3.61 PSMNEE227 pKa = 3.34 NTRR230 pKa = 11.84 YY231 pKa = 9.36 YY232 pKa = 11.08 GSYY235 pKa = 9.74 TGGQSTPPVLQFTNTVTTVLLDD257 pKa = 3.84 EE258 pKa = 5.12 NGVGPLCKK266 pKa = 10.13 GDD268 pKa = 4.16 ALYY271 pKa = 8.95 LTAADD276 pKa = 2.74 IVGFHH281 pKa = 6.32 TNAAEE286 pKa = 3.98 TMAYY290 pKa = 9.9 RR291 pKa = 11.84 GLPRR295 pKa = 11.84 YY296 pKa = 10.01 FNVTLRR302 pKa = 11.84 KK303 pKa = 9.36 RR304 pKa = 11.84 AVKK307 pKa = 10.4 NPYY310 pKa = 9.15 PVSTLLNNLFTGYY323 pKa = 9.98 LPKK326 pKa = 10.56 VEE328 pKa = 4.18 GQPMNDD334 pKa = 3.13 KK335 pKa = 10.66 NGEE338 pKa = 4.03 VGQVEE343 pKa = 4.47 EE344 pKa = 4.15 VRR346 pKa = 11.84 VYY348 pKa = 11.12 EE349 pKa = 4.42 GMEE352 pKa = 4.1 PTPGDD357 pKa = 3.56 PDD359 pKa = 3.76 MIRR362 pKa = 11.84 YY363 pKa = 9.33 LDD365 pKa = 3.61 EE366 pKa = 5.19 FGQQKK371 pKa = 9.02 TSPNMNN377 pKa = 3.48
Molecular weight: 41.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.571
IPC2_protein 6.781
IPC_protein 6.883
Toseland 6.605
ProMoST 7.278
Dawson 7.527
Bjellqvist 7.512
Wikipedia 7.454
Rodwell 7.512
Grimsley 6.634
Solomon 7.6
Lehninger 7.614
Nozaki 7.775
DTASelect 7.644
Thurlkill 7.673
EMBOSS 7.761
Sillero 7.936
Patrickios 4.024
IPC_peptide 7.585
IPC2_peptide 6.971
IPC2.peptide.svr19 7.227
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1584
197
697
396.0
44.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.503 ± 0.814
2.525 ± 0.648
5.366 ± 0.394
6.755 ± 0.368
4.293 ± 0.794
6.692 ± 0.677
2.146 ± 0.392
4.419 ± 0.599
6.692 ± 1.15
9.848 ± 0.482
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.093 ± 0.366
4.545 ± 0.837
5.808 ± 1.006
3.535 ± 0.094
4.293 ± 0.417
6.566 ± 0.876
5.24 ± 0.839
6.187 ± 0.635
1.515 ± 0.397
3.977 ± 0.561
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here