Eptesicus serotinus papillomavirus 2
Average proteome isoelectric point is 5.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W8EC56|W8EC56_9PAPI Protein E6 OS=Eptesicus serotinus papillomavirus 2 OX=1464072 GN=E6 PE=3 SV=1
MM1 pKa = 7.56 IGNAPTLKK9 pKa = 10.64 DD10 pKa = 3.18 IVLTEE15 pKa = 3.8 IPEE18 pKa = 4.3 AVDD21 pKa = 3.94 LLCHH25 pKa = 5.38 EE26 pKa = 4.68 TMPSEE31 pKa = 4.36 EE32 pKa = 4.15 EE33 pKa = 3.91 LGAGDD38 pKa = 3.54 SHH40 pKa = 8.32 SGNRR44 pKa = 11.84 PRR46 pKa = 11.84 AQQVHH51 pKa = 6.8 VILTWCGRR59 pKa = 11.84 CDD61 pKa = 3.27 QDD63 pKa = 3.89 IRR65 pKa = 11.84 VCIEE69 pKa = 3.79 TEE71 pKa = 3.97 QQSLVILEE79 pKa = 4.16 SLLLKK84 pKa = 10.34 DD85 pKa = 4.72 LQILCPEE92 pKa = 5.23 CYY94 pKa = 9.97 QNLL97 pKa = 3.69
Molecular weight: 10.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.124
IPC2_protein 4.444
IPC_protein 4.317
Toseland 4.177
ProMoST 4.431
Dawson 4.266
Bjellqvist 4.418
Wikipedia 4.139
Rodwell 4.164
Grimsley 4.088
Solomon 4.266
Lehninger 4.215
Nozaki 4.38
DTASelect 4.507
Thurlkill 4.19
EMBOSS 4.164
Sillero 4.431
Patrickios 1.99
IPC_peptide 4.266
IPC2_peptide 4.431
IPC2.peptide.svr19 4.319
Protein with the highest isoelectric point:
>tr|W8EH55|W8EH55_9PAPI Regulatory protein E2 OS=Eptesicus serotinus papillomavirus 2 OX=1464072 GN=E2 PE=3 SV=1
MM1 pKa = 7.46 KK2 pKa = 10.25 RR3 pKa = 11.84 RR4 pKa = 11.84 EE5 pKa = 4.1 TTEE8 pKa = 3.74 QLTSRR13 pKa = 11.84 LDD15 pKa = 3.48 AVQEE19 pKa = 4.1 KK20 pKa = 10.18 ILAHH24 pKa = 6.48 FEE26 pKa = 4.26 TTSTEE31 pKa = 3.84 LAAHH35 pKa = 6.34 IEE37 pKa = 4.17 YY38 pKa = 9.68 WSLVRR43 pKa = 11.84 QEE45 pKa = 4.91 SVILYY50 pKa = 6.66 YY51 pKa = 10.67 ARR53 pKa = 11.84 HH54 pKa = 6.05 LGVNRR59 pKa = 11.84 LGLQPVPAQAISEE72 pKa = 4.15 GRR74 pKa = 11.84 AKK76 pKa = 10.16 QAIGMQLVLEE86 pKa = 4.27 SLQRR90 pKa = 11.84 SPYY93 pKa = 10.36 ARR95 pKa = 11.84 EE96 pKa = 3.39 EE97 pKa = 3.82 WTLQDD102 pKa = 3.49 VSLEE106 pKa = 4.22 KK107 pKa = 11.15 YY108 pKa = 10.13 SVQPKK113 pKa = 8.58 NTFKK117 pKa = 11.03 KK118 pKa = 10.45 DD119 pKa = 3.08 GTQVHH124 pKa = 5.8 VKK126 pKa = 10.18 YY127 pKa = 10.86 GPDD130 pKa = 3.3 PTDD133 pKa = 3.77 FMPYY137 pKa = 9.77 VLWGSIYY144 pKa = 10.69 YY145 pKa = 10.02 QDD147 pKa = 5.82 VDD149 pKa = 4.52 DD150 pKa = 4.97 KK151 pKa = 9.07 WHH153 pKa = 6.45 KK154 pKa = 11.03 VPGQVDD160 pKa = 3.53 YY161 pKa = 11.49 HH162 pKa = 7.07 GLFYY166 pKa = 10.61 MEE168 pKa = 4.6 GKK170 pKa = 10.12 EE171 pKa = 4.03 KK172 pKa = 9.89 IYY174 pKa = 10.96 YY175 pKa = 10.39 EE176 pKa = 4.09 NFKK179 pKa = 10.88 EE180 pKa = 4.0 DD181 pKa = 3.86 AIKK184 pKa = 10.62 FGMPGRR190 pKa = 11.84 WIVNVKK196 pKa = 8.4 NTAVPSFSRR205 pKa = 11.84 QTSSAFAGGRR215 pKa = 11.84 PGADD219 pKa = 2.94 STDD222 pKa = 3.47 APEE225 pKa = 4.61 APSTSRR231 pKa = 11.84 WSPRR235 pKa = 11.84 ASNTTQAQEE244 pKa = 4.34 SNQVSGPSTRR254 pKa = 11.84 RR255 pKa = 11.84 GRR257 pKa = 11.84 GRR259 pKa = 11.84 RR260 pKa = 11.84 SPSPGEE266 pKa = 4.26 GPSSSRR272 pKa = 11.84 RR273 pKa = 11.84 RR274 pKa = 11.84 RR275 pKa = 11.84 LPTAARR281 pKa = 11.84 RR282 pKa = 11.84 TPSPEE287 pKa = 3.2 RR288 pKa = 11.84 HH289 pKa = 5.36 SRR291 pKa = 11.84 SRR293 pKa = 11.84 SRR295 pKa = 11.84 DD296 pKa = 2.81 WRR298 pKa = 11.84 GGRR301 pKa = 11.84 GGGGGLRR308 pKa = 11.84 GRR310 pKa = 11.84 GRR312 pKa = 11.84 QGEE315 pKa = 4.44 SAGRR319 pKa = 11.84 SAGPHH324 pKa = 5.39 SRR326 pKa = 11.84 SSQSPAPTPPTPGQVGGRR344 pKa = 11.84 HH345 pKa = 4.63 RR346 pKa = 11.84 TAEE349 pKa = 4.22 PGASRR354 pKa = 11.84 LEE356 pKa = 4.01 RR357 pKa = 11.84 LIQEE361 pKa = 4.31 ARR363 pKa = 11.84 DD364 pKa = 3.7 PPVVLIRR371 pKa = 11.84 GTPNGVKK378 pKa = 10.2 CLRR381 pKa = 11.84 YY382 pKa = 9.56 RR383 pKa = 11.84 LRR385 pKa = 11.84 TQNQHH390 pKa = 6.65 LYY392 pKa = 10.99 DD393 pKa = 4.01 SVTSTFFWVNKK404 pKa = 9.72 NGKK407 pKa = 6.35 QQEE410 pKa = 4.23 GRR412 pKa = 11.84 IMLSFTGTPQRR423 pKa = 11.84 THH425 pKa = 6.25 FLSVFKK431 pKa = 10.83 VPKK434 pKa = 10.39 GMSLSLGRR442 pKa = 11.84 LDD444 pKa = 4.94 ALL446 pKa = 4.35
Molecular weight: 49.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.314
IPC2_protein 9.663
IPC_protein 10.423
Toseland 10.482
ProMoST 10.204
Dawson 10.628
Bjellqvist 10.335
Wikipedia 10.847
Rodwell 10.833
Grimsley 10.701
Solomon 10.701
Lehninger 10.672
Nozaki 10.452
DTASelect 10.335
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.555
Patrickios 10.482
IPC_peptide 10.701
IPC2_peptide 9.151
IPC2.peptide.svr19 8.711
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2343
97
620
390.5
43.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.719 ± 0.347
2.091 ± 0.805
6.274 ± 0.649
6.018 ± 0.513
4.225 ± 0.504
7.896 ± 1.159
2.006 ± 0.177
4.14 ± 0.638
4.14 ± 0.851
8.792 ± 0.82
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.494 ± 0.31
3.286 ± 0.685
6.701 ± 0.881
4.78 ± 0.586
7.085 ± 0.913
7.085 ± 0.738
6.701 ± 0.518
7.128 ± 0.916
1.28 ± 0.348
3.158 ± 0.371
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here