Duganella ginsengisoli
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5918 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6L6Q9A2|A0A6L6Q9A2_9BURK Uncharacterized protein OS=Duganella ginsengisoli OX=1462440 GN=GM668_29535 PE=4 SV=1
MM1 pKa = 7.86 SDD3 pKa = 3.39 LTAPSLKK10 pKa = 10.52 SVTFTNSTANASAVNTSADD29 pKa = 3.09 IVLTFSEE36 pKa = 4.78 AMTSGTGLITISDD49 pKa = 4.49 GATQTYY55 pKa = 8.45 MGKK58 pKa = 10.2 DD59 pKa = 2.98 GTLRR63 pKa = 11.84 TRR65 pKa = 11.84 LVGATDD71 pKa = 3.26 TRR73 pKa = 11.84 TVDD76 pKa = 3.62 ISDD79 pKa = 3.8 TSQVTVSGDD88 pKa = 3.24 TVTINLASDD97 pKa = 3.96 LKK99 pKa = 10.78 AGKK102 pKa = 9.89 SYY104 pKa = 10.56 SVQMGKK110 pKa = 10.57 GVLLDD115 pKa = 3.78 EE116 pKa = 5.65 AGNAYY121 pKa = 10.31 AGLSDD126 pKa = 3.5 TTKK129 pKa = 11.15 LKK131 pKa = 9.6 FTTSAATLPTAVVDD145 pKa = 3.86 STFTITDD152 pKa = 3.69 TGSSASDD159 pKa = 4.47 FITSSAVQTIAGKK172 pKa = 10.95 VNGALLAGEE181 pKa = 4.42 AVMVSLDD188 pKa = 4.74 NGGTWAAATVNAADD202 pKa = 4.14 STWSIGGTVTGSSTIIARR220 pKa = 11.84 VVNAEE225 pKa = 4.09 GNASAAARR233 pKa = 11.84 HH234 pKa = 6.32 AYY236 pKa = 9.06 TFDD239 pKa = 3.25 TDD241 pKa = 3.38 APTVGSIAINDD252 pKa = 3.68 TDD254 pKa = 4.12 LVAGEE259 pKa = 4.32 TATVTVTFNEE269 pKa = 4.36 AVTGLDD275 pKa = 3.13 VSDD278 pKa = 3.95 FTVEE282 pKa = 4.03 GGALSSLATADD293 pKa = 4.31 NGKK296 pKa = 7.63 TWTATLTPTKK306 pKa = 10.29 GVSTTTGTVTLNAGSVADD324 pKa = 4.0 LAGNSGPANSSSKK337 pKa = 9.78 PFNYY341 pKa = 8.93 STVPDD346 pKa = 4.14 APTATVTSISMTDD359 pKa = 3.3 TGSSATDD366 pKa = 4.37 FITNSTSQTIKK377 pKa = 9.49 GTYY380 pKa = 9.73 SGTLAADD387 pKa = 4.28 EE388 pKa = 4.75 YY389 pKa = 10.97 IQVSLDD395 pKa = 3.7 NGSTWTKK402 pKa = 9.82 ATVSGSNWEE411 pKa = 4.12 VAGATISASNTLKK424 pKa = 10.79 ARR426 pKa = 11.84 VFNDD430 pKa = 3.19 YY431 pKa = 10.2 TYY433 pKa = 11.08 SNPMAQAYY441 pKa = 7.86 TLDD444 pKa = 3.55 QTAPTATVTLDD455 pKa = 3.09 KK456 pKa = 11.42 AHH458 pKa = 6.61 LKK460 pKa = 10.47 KK461 pKa = 11.16 GEE463 pKa = 4.17 TATITITFSEE473 pKa = 4.79 AVTGLTSADD482 pKa = 3.03 IKK484 pKa = 11.62 SNDD487 pKa = 3.65 TVGTPVSTDD496 pKa = 3.05 GGVTWTATLTPTNNGNGSVTLYY518 pKa = 10.76 ASSVTDD524 pKa = 3.5 LAGNSGPAADD534 pKa = 3.82 VAATYY539 pKa = 10.78 SSDD542 pKa = 3.35 LTRR545 pKa = 11.84 PTATISLADD554 pKa = 3.5 SALTAGEE561 pKa = 4.36 TTLVTITFSEE571 pKa = 4.44 AVTEE575 pKa = 4.19 FTNADD580 pKa = 3.6 LTVGNGTLSTVTSTDD595 pKa = 3.2 GGTTWTATLTPTASTTATTNVITLDD620 pKa = 3.47 NTAVSDD626 pKa = 3.84 LAGNTGIGSTSSANYY641 pKa = 9.31 SVSTVRR647 pKa = 11.84 PTATINIADD656 pKa = 4.02 TALTAGEE663 pKa = 4.41 TTTVTFTFSSAVTGFDD679 pKa = 3.3 ASDD682 pKa = 3.29 VTVANGTISTPTTTDD697 pKa = 3.13 NITWTATLTPSASTEE712 pKa = 3.85 DD713 pKa = 3.56 TTNVITLANTGFHH726 pKa = 6.5 NSAGNSGTGTTTSGNYY742 pKa = 8.72 TVDD745 pKa = 3.35 TARR748 pKa = 11.84 PTATISLADD757 pKa = 3.49 TALTVGEE764 pKa = 4.51 TTTVTVKK771 pKa = 10.46 FSEE774 pKa = 4.31 AVTGFSASSLSVANGTISSLTSSDD798 pKa = 3.47 NITWTGTLTPTASQEE813 pKa = 3.91 DD814 pKa = 4.19 STNVITLNNSGVSDD828 pKa = 3.61 AAGNTGSGTTTSANYY843 pKa = 9.02 TVDD846 pKa = 3.31 TLRR849 pKa = 11.84 PTATISLADD858 pKa = 3.5 SALTAGEE865 pKa = 4.35 TTTVSITFSEE875 pKa = 5.01 VVTGLTTADD884 pKa = 3.35 LTVANGTLTSLQSSDD899 pKa = 3.04 GMNWTATLTPSASTDD914 pKa = 3.14 DD915 pKa = 3.77 TTNVITLDD923 pKa = 3.35 NTGITDD929 pKa = 3.63 ASGNAGSGTTTSANYY944 pKa = 8.53 TVATVRR950 pKa = 11.84 PTATIAVSDD959 pKa = 4.02 TALKK963 pKa = 9.91 TGDD966 pKa = 3.6 TATVTITFSEE976 pKa = 4.55 AVTGLTAADD985 pKa = 3.35 FTVANGTLSTPTTTDD1000 pKa = 3.09 NITWTATLTPTSSIEE1015 pKa = 3.9 DD1016 pKa = 3.71 TTNVITLDD1024 pKa = 3.54 NTGVTDD1030 pKa = 3.43 SDD1032 pKa = 4.3 GNTGSGTTSSSNYY1045 pKa = 9.21 TVDD1048 pKa = 3.26 TKK1050 pKa = 11.59 APTATIALSQPEE1062 pKa = 4.87 LLTGEE1067 pKa = 4.36 TSTVTITFNEE1077 pKa = 4.26 AVTGLTLADD1086 pKa = 4.31 FKK1088 pKa = 11.72 YY1089 pKa = 10.16 SGTMTAPVSTDD1100 pKa = 3.26 GGTTWVATLTPSTEE1114 pKa = 4.09 SSSGEE1119 pKa = 3.72 VRR1121 pKa = 11.84 LNTSSVTDD1129 pKa = 3.37 SAGNSGPSSEE1139 pKa = 5.13 AYY1141 pKa = 10.35 ASFTYY1146 pKa = 10.35 NAAPTATIATASITDD1161 pKa = 3.64 TGTSGDD1167 pKa = 4.69 FITSSTSQEE1176 pKa = 3.56 ISGTLSAALGRR1187 pKa = 11.84 GQYY1190 pKa = 9.81 VQVSLDD1196 pKa = 3.59 NGEE1199 pKa = 4.36 SWTTATTIGTGWSLSGQTISHH1220 pKa = 6.52 SSTLKK1225 pKa = 9.98 VRR1227 pKa = 11.84 ATNGSSTSTTYY1238 pKa = 10.48 SHH1240 pKa = 7.41 SYY1242 pKa = 9.6 TLDD1245 pKa = 3.04 QAAPTAPTITLSKK1258 pKa = 10.57 DD1259 pKa = 3.08 ALYY1262 pKa = 10.33 TNQTATVTVVFDD1274 pKa = 3.44 EE1275 pKa = 4.4 AVYY1278 pKa = 10.95 GLTASDD1284 pKa = 3.62 FTISGGTLGTFSTSDD1299 pKa = 3.33 NLTWTATLTPTATGSLALAASSVYY1323 pKa = 9.49 DD1324 pKa = 3.3 TAGNVGPTSASSSKK1338 pKa = 10.94 SFTFTADD1345 pKa = 3.1 TTGPTATLTLNDD1357 pKa = 3.44 NTLGKK1362 pKa = 10.69 GGTATLTVTLSEE1374 pKa = 4.6 AVTDD1378 pKa = 3.99 TPTASDD1384 pKa = 3.7 FTVSGGSLGTFTSTDD1399 pKa = 3.07 SGLTWTATLTPTDD1412 pKa = 3.54 SAYY1415 pKa = 10.5 GAGTISLSANAISDD1429 pKa = 3.5 QSGNTGPSSAASLPFYY1445 pKa = 10.9 YY1446 pKa = 9.36 DD1447 pKa = 3.27 TQALTLSSSIAFSTDD1462 pKa = 2.79 SGEE1465 pKa = 4.31 SGSDD1469 pKa = 3.68 LITNTGTQTISGTYY1483 pKa = 9.53 SGGTIYY1489 pKa = 10.04 STMGDD1494 pKa = 3.82 KK1495 pKa = 10.7 IEE1497 pKa = 4.01 VSTDD1501 pKa = 3.07 NGSTWSEE1508 pKa = 3.83 ATVSGQAWSISKK1520 pKa = 8.57 TLSGSDD1526 pKa = 3.61 TIQVRR1531 pKa = 11.84 LTDD1534 pKa = 3.76 GTGVAGTPVSHH1545 pKa = 7.29 TYY1547 pKa = 10.22 TIDD1550 pKa = 3.33 TTSPGSMSSKK1560 pKa = 10.39 IPDD1563 pKa = 3.7 LASSSDD1569 pKa = 3.28 TSNGVSGGTSDD1580 pKa = 6.36 DD1581 pKa = 3.42 ITSNTTPVITYY1592 pKa = 10.16 SLYY1595 pKa = 11.07 NSGLTAGDD1603 pKa = 3.75 YY1604 pKa = 11.15 VDD1606 pKa = 5.57 VYY1608 pKa = 11.09 DD1609 pKa = 4.31 TTTSTILHH1617 pKa = 5.71 THH1619 pKa = 7.28 TITSSEE1625 pKa = 4.04 LTSYY1629 pKa = 10.99 GGTVSATVSEE1639 pKa = 4.85 LSAGSHH1645 pKa = 5.06 SLVLRR1650 pKa = 11.84 SRR1652 pKa = 11.84 DD1653 pKa = 3.34 AAGNTGTQSQALALTIDD1670 pKa = 3.94 TSAISMAGKK1679 pKa = 8.12 TVDD1682 pKa = 4.07 LAASSDD1688 pKa = 3.84 SGTSSTDD1695 pKa = 3.14 NITNNSAPTVTVNVANTSGLVAGDD1719 pKa = 3.59 KK1720 pKa = 10.83 LQIIDD1725 pKa = 4.35 SSNGDD1730 pKa = 3.64 AVVGTYY1736 pKa = 9.34 TLTSTDD1742 pKa = 4.05 FSSSGADD1749 pKa = 3.13 INITLSTLTDD1759 pKa = 3.51 GTHH1762 pKa = 6.55 ALALRR1767 pKa = 11.84 VYY1769 pKa = 10.56 DD1770 pKa = 3.6 RR1771 pKa = 11.84 AGNTGTASSATSITIDD1787 pKa = 3.81 TIAPSLSSSTPSSSSTSNSVSTSSVTLVFSEE1818 pKa = 4.54 ALADD1822 pKa = 3.79 VNSRR1826 pKa = 11.84 TGFKK1830 pKa = 9.55 VTDD1833 pKa = 3.86 GANDD1837 pKa = 3.17 ARR1839 pKa = 11.84 EE1840 pKa = 4.15 ITGEE1844 pKa = 3.97 ALSGNTLSYY1853 pKa = 11.2 DD1854 pKa = 3.71 SSTHH1858 pKa = 5.29 TATLSLSGSLQYY1870 pKa = 11.47 ASTYY1874 pKa = 8.08 TVYY1877 pKa = 10.73 VYY1879 pKa = 10.91 NAALSDD1885 pKa = 3.56 TAGNSVAIDD1894 pKa = 3.44 TALLSFSTEE1903 pKa = 4.24 SVPTPSAVTLAMTEE1917 pKa = 4.0 STYY1920 pKa = 11.33 ASHH1923 pKa = 7.16 TGTDD1927 pKa = 3.42 NDD1929 pKa = 5.27 HH1930 pKa = 6.35 ITNNATLCVSGITAAYY1946 pKa = 9.69 LRR1948 pKa = 11.84 YY1949 pKa = 10.06 KK1950 pKa = 9.49 LTSNAGWTTVQTSGSTYY1967 pKa = 10.41 SIPLSDD1973 pKa = 3.97 DD1974 pKa = 3.24 TYY1976 pKa = 10.67 AAGVIQVEE1984 pKa = 4.6 QYY1986 pKa = 8.96 DD1987 pKa = 3.95 TAGYY1991 pKa = 9.77 EE1992 pKa = 4.13 STAATLSDD2000 pKa = 3.47 SWTVDD2005 pKa = 2.83 TSSPSGRR2012 pKa = 11.84 ISTSTSGLPSFTGLGINTAISTITGSVSGEE2042 pKa = 3.87 FNLDD2046 pKa = 3.5 DD2047 pKa = 4.26 EE2048 pKa = 5.28 FIEE2051 pKa = 4.38 YY2052 pKa = 7.59 TTDD2055 pKa = 4.69 SGSTWTKK2062 pKa = 10.18 ASAMTAFIWTIADD2075 pKa = 3.39 VKK2077 pKa = 10.34 VAAGGEE2083 pKa = 3.94 IGFRR2087 pKa = 11.84 ASDD2090 pKa = 3.16 KK2091 pKa = 11.0 AGNIGYY2097 pKa = 10.03 YY2098 pKa = 10.75 GEE2100 pKa = 4.16 TSNYY2104 pKa = 7.35 TVYY2107 pKa = 11.01 VGDD2110 pKa = 4.61 DD2111 pKa = 3.06 SAGNHH2116 pKa = 5.57 TATGSKK2122 pKa = 10.34 YY2123 pKa = 10.69 LYY2125 pKa = 8.71 TQAGNDD2131 pKa = 3.89 TIDD2134 pKa = 3.0 ITGASNAVHH2143 pKa = 6.81 AGSGNDD2149 pKa = 3.85 AININSASTTNHH2161 pKa = 5.5 VYY2163 pKa = 11.14 GDD2165 pKa = 4.07 GGSDD2169 pKa = 3.55 TINVNGSHH2177 pKa = 6.68 NSQIEE2182 pKa = 4.07 GGADD2186 pKa = 3.34 ADD2188 pKa = 4.56 TITVTGSYY2196 pKa = 10.64 NYY2198 pKa = 10.38 VYY2200 pKa = 10.98 GNDD2203 pKa = 3.78 GDD2205 pKa = 4.25 DD2206 pKa = 3.6 TLSISGTYY2214 pKa = 7.9 NTVYY2218 pKa = 10.82 GDD2220 pKa = 3.51 NSEE2223 pKa = 4.33 TGDD2226 pKa = 3.6 TGADD2230 pKa = 3.63 TITVSGSSNTVDD2242 pKa = 3.87 AGPGANSITVTGADD2256 pKa = 3.31 NTVTGGSDD2264 pKa = 3.1 TDD2266 pKa = 4.18 TISVSSTGNTVYY2278 pKa = 10.84 GGDD2281 pKa = 3.65 SADD2284 pKa = 4.02 TITVATSGNTIYY2296 pKa = 11.23 GEE2298 pKa = 4.15 AGADD2302 pKa = 3.52 QFTVAALGSNIYY2314 pKa = 10.67 GGDD2317 pKa = 3.55 GSDD2320 pKa = 3.33 TFTVTASLAGTLSGLIDD2337 pKa = 4.31 GGTDD2341 pKa = 3.18 GTDD2344 pKa = 3.16 VLNIGVSSQTLSLSTLTSNIAHH2366 pKa = 6.91 IEE2368 pKa = 4.14 KK2369 pKa = 10.33 ISLGTSNTLTISGAAIQTMSDD2390 pKa = 2.89 TDD2392 pKa = 4.0 SIRR2395 pKa = 11.84 FEE2397 pKa = 4.47 GDD2399 pKa = 3.13 STDD2402 pKa = 3.35 TIYY2405 pKa = 11.33 YY2406 pKa = 10.3 DD2407 pKa = 3.33 SSLWEE2412 pKa = 4.02 SATWIQILGYY2422 pKa = 10.35 NKK2424 pKa = 10.12 YY2425 pKa = 8.42 QLKK2428 pKa = 10.49 SDD2430 pKa = 3.56 TTITFIIDD2438 pKa = 3.45 SDD2440 pKa = 3.31 IHH2442 pKa = 8.31 LYY2444 pKa = 11.12 GLTSS2448 pKa = 3.21
Molecular weight: 246.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.672
IPC2_protein 3.681
IPC_protein 3.757
Toseland 3.516
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.719
Rodwell 3.567
Grimsley 3.414
Solomon 3.757
Lehninger 3.719
Nozaki 3.859
DTASelect 4.177
Thurlkill 3.567
EMBOSS 3.719
Sillero 3.884
Patrickios 1.074
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.77
Protein with the highest isoelectric point:
>tr|A0A6L6Q7R1|A0A6L6Q7R1_9BURK Uncharacterized protein OS=Duganella ginsengisoli OX=1462440 GN=GM668_27725 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 9.97 QPSVVRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.57 RR14 pKa = 11.84 THH16 pKa = 5.79 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATRR25 pKa = 11.84 GGRR28 pKa = 11.84 QVLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.65 GRR39 pKa = 11.84 KK40 pKa = 8.72 RR41 pKa = 11.84 LAVVV45 pKa = 3.61
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.447
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.1
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5918
0
5918
2124225
37
12803
358.9
38.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.019 ± 0.053
0.883 ± 0.011
5.416 ± 0.023
4.965 ± 0.034
3.404 ± 0.02
8.152 ± 0.041
2.235 ± 0.019
4.56 ± 0.025
3.676 ± 0.031
10.305 ± 0.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.638 ± 0.018
3.149 ± 0.025
4.916 ± 0.03
4.261 ± 0.028
6.155 ± 0.042
5.571 ± 0.036
5.439 ± 0.05
7.301 ± 0.034
1.325 ± 0.014
2.629 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here