Corynebacterium riegelii
Average proteome isoelectric point is 5.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2204 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0K1RDN4|A0A0K1RDN4_9CORY Catalase OS=Corynebacterium riegelii OX=156976 GN=AK829_09170 PE=3 SV=1
MM1 pKa = 7.51 NKK3 pKa = 9.3 QAMASVVAAGVATVFILAGAFALLRR28 pKa = 11.84 GGPDD32 pKa = 3.32 GGPDD36 pKa = 3.23 ASLASSFDD44 pKa = 3.38 PSMSVQRR51 pKa = 11.84 SEE53 pKa = 4.32 VMAPAAEE60 pKa = 4.19 YY61 pKa = 10.69 SGNRR65 pKa = 11.84 PDD67 pKa = 4.28 CVAGGVGGVDD77 pKa = 4.15 LPCLGGAEE85 pKa = 4.81 DD86 pKa = 4.51 PGQFADD92 pKa = 3.67 VTLVNVWAWWCVPCRR107 pKa = 11.84 EE108 pKa = 4.5 EE109 pKa = 6.26 LPVLDD114 pKa = 3.79 QFARR118 pKa = 11.84 EE119 pKa = 3.97 HH120 pKa = 6.69 PEE122 pKa = 3.4 ISVVGVHH129 pKa = 6.93 ADD131 pKa = 3.29 ANAEE135 pKa = 4.01 NGIALLDD142 pKa = 4.11 EE143 pKa = 5.42 LDD145 pKa = 3.54 VSYY148 pKa = 10.7 PSYY151 pKa = 11.17 QDD153 pKa = 4.28 DD154 pKa = 3.62 SGKK157 pKa = 10.37 FAGQLALPNVVPLLLVYY174 pKa = 10.54 KK175 pKa = 10.43 DD176 pKa = 3.84 GEE178 pKa = 4.49 QVGVYY183 pKa = 8.26 PQTYY187 pKa = 9.81 ASVEE191 pKa = 4.02 EE192 pKa = 4.36 LEE194 pKa = 4.58 ALIGQVNN201 pKa = 3.38
Molecular weight: 20.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.741
IPC2_protein 3.923
IPC_protein 3.884
Toseland 3.681
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.592
Solomon 3.846
Lehninger 3.808
Nozaki 3.973
DTASelect 4.177
Thurlkill 3.732
EMBOSS 3.795
Sillero 3.999
Patrickios 1.036
IPC_peptide 3.846
IPC2_peptide 3.986
IPC2.peptide.svr19 3.874
Protein with the highest isoelectric point:
>tr|A0A0K1RDY9|A0A0K1RDY9_9CORY Uncharacterized protein OS=Corynebacterium riegelii OX=156976 GN=AK829_09940 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 SRR15 pKa = 11.84 KK16 pKa = 8.14 HH17 pKa = 4.83 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AIISARR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.49 KK38 pKa = 10.52 GRR40 pKa = 11.84 AKK42 pKa = 10.73 LSAA45 pKa = 3.92
Molecular weight: 5.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.568
Rodwell 12.735
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.457
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.237
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2204
0
2204
716373
40
3017
325.0
35.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.914 ± 0.069
0.667 ± 0.013
6.014 ± 0.046
6.493 ± 0.055
3.385 ± 0.035
8.228 ± 0.054
2.076 ± 0.024
4.863 ± 0.037
3.263 ± 0.044
9.439 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.29 ± 0.023
2.955 ± 0.032
5.108 ± 0.036
3.509 ± 0.033
6.179 ± 0.05
5.344 ± 0.032
6.203 ± 0.041
8.405 ± 0.047
1.38 ± 0.022
2.282 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here