Amino acid dipepetide frequency for Gluconobacter phage GC1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.917AlaAla: 11.917 ± 2.353
0.794AlaCys: 0.794 ± 0.361
4.965AlaAsp: 4.965 ± 1.096
6.753AlaGlu: 6.753 ± 1.532
3.972AlaPhe: 3.972 ± 0.947
6.753AlaGly: 6.753 ± 0.875
1.787AlaHis: 1.787 ± 0.676
6.157AlaIle: 6.157 ± 1.143
4.369AlaLys: 4.369 ± 1.059
9.93AlaLeu: 9.93 ± 1.471
2.781AlaMet: 2.781 ± 0.762
3.178AlaAsn: 3.178 ± 0.824
3.575AlaPro: 3.575 ± 0.629
3.774AlaGln: 3.774 ± 1.026
3.972AlaArg: 3.972 ± 0.747
9.533AlaSer: 9.533 ± 1.455
8.342AlaThr: 8.342 ± 1.478
6.753AlaVal: 6.753 ± 1.279
1.192AlaTrp: 1.192 ± 0.539
2.979AlaTyr: 2.979 ± 0.623
0.0AlaXaa: 0.0 ± 0.0
Cys
0.794CysAla: 0.794 ± 0.424
0.199CysCys: 0.199 ± 0.181
1.192CysAsp: 1.192 ± 0.409
0.794CysGlu: 0.794 ± 0.477
0.397CysPhe: 0.397 ± 0.293
0.397CysGly: 0.397 ± 0.305
0.199CysHis: 0.199 ± 0.181
0.794CysIle: 0.794 ± 0.438
0.0CysLys: 0.0 ± 0.0
0.596CysLeu: 0.596 ± 0.271
0.199CysMet: 0.199 ± 0.163
0.397CysAsn: 0.397 ± 0.294
0.199CysPro: 0.199 ± 0.223
0.397CysGln: 0.397 ± 0.278
0.199CysArg: 0.199 ± 0.166
0.0CysSer: 0.0 ± 0.0
0.794CysThr: 0.794 ± 0.508
0.794CysVal: 0.794 ± 0.381
0.0CysTrp: 0.0 ± 0.0
0.794CysTyr: 0.794 ± 0.507
0.0CysXaa: 0.0 ± 0.0
Asp
6.157AspAla: 6.157 ± 1.17
0.993AspCys: 0.993 ± 0.567
4.171AspAsp: 4.171 ± 0.957
3.178AspGlu: 3.178 ± 1.024
3.972AspPhe: 3.972 ± 1.005
4.965AspGly: 4.965 ± 1.092
0.596AspHis: 0.596 ± 0.311
3.178AspIle: 3.178 ± 0.62
1.192AspLys: 1.192 ± 0.469
2.582AspLeu: 2.582 ± 0.494
1.787AspMet: 1.787 ± 0.53
1.986AspAsn: 1.986 ± 0.603
3.178AspPro: 3.178 ± 0.702
1.192AspGln: 1.192 ± 0.447
3.178AspArg: 3.178 ± 0.772
2.979AspSer: 2.979 ± 0.739
4.767AspThr: 4.767 ± 1.274
2.979AspVal: 2.979 ± 0.724
0.596AspTrp: 0.596 ± 0.256
1.39AspTyr: 1.39 ± 0.751
0.0AspXaa: 0.0 ± 0.0
Glu
5.76GluAla: 5.76 ± 1.356
0.397GluCys: 0.397 ± 0.299
3.178GluAsp: 3.178 ± 0.757
4.369GluGlu: 4.369 ± 1.399
2.781GluPhe: 2.781 ± 0.82
3.575GluGly: 3.575 ± 0.841
1.192GluHis: 1.192 ± 0.559
3.774GluIle: 3.774 ± 0.956
4.767GluLys: 4.767 ± 1.253
3.774GluLeu: 3.774 ± 1.028
3.178GluMet: 3.178 ± 0.618
3.575GluAsn: 3.575 ± 0.868
0.596GluPro: 0.596 ± 0.285
1.787GluGln: 1.787 ± 0.618
3.376GluArg: 3.376 ± 1.041
2.582GluSer: 2.582 ± 0.74
3.376GluThr: 3.376 ± 1.275
1.986GluVal: 1.986 ± 0.649
0.794GluTrp: 0.794 ± 0.592
0.794GluTyr: 0.794 ± 0.316
0.0GluXaa: 0.0 ± 0.0
Phe
4.965PheAla: 4.965 ± 0.845
0.397PheCys: 0.397 ± 0.273
2.582PheAsp: 2.582 ± 0.888
1.589PheGlu: 1.589 ± 0.685
1.589PhePhe: 1.589 ± 0.613
3.376PheGly: 3.376 ± 0.739
0.794PheHis: 0.794 ± 0.384
1.39PheIle: 1.39 ± 0.481
1.986PheLys: 1.986 ± 0.581
3.575PheLeu: 3.575 ± 0.781
1.589PheMet: 1.589 ± 0.59
1.787PheAsn: 1.787 ± 0.775
2.185PhePro: 2.185 ± 0.556
0.397PheGln: 0.397 ± 0.238
1.986PheArg: 1.986 ± 0.56
2.582PheSer: 2.582 ± 0.719
2.185PheThr: 2.185 ± 0.839
3.972PheVal: 3.972 ± 0.671
0.596PheTrp: 0.596 ± 0.331
0.993PheTyr: 0.993 ± 0.538
0.0PheXaa: 0.0 ± 0.0
Gly
7.746GlyAla: 7.746 ± 1.28
0.0GlyCys: 0.0 ± 0.0
4.369GlyAsp: 4.369 ± 0.635
3.972GlyGlu: 3.972 ± 0.913
3.376GlyPhe: 3.376 ± 0.799
5.76GlyGly: 5.76 ± 1.249
0.596GlyHis: 0.596 ± 0.283
6.356GlyIle: 6.356 ± 1.231
4.568GlyLys: 4.568 ± 1.154
4.965GlyLeu: 4.965 ± 0.913
2.979GlyMet: 2.979 ± 0.814
4.568GlyAsn: 4.568 ± 1.032
1.986GlyPro: 1.986 ± 0.644
3.376GlyGln: 3.376 ± 0.787
3.575GlyArg: 3.575 ± 1.047
5.76GlySer: 5.76 ± 1.288
4.965GlyThr: 4.965 ± 1.739
3.972GlyVal: 3.972 ± 0.797
1.787GlyTrp: 1.787 ± 0.828
3.575GlyTyr: 3.575 ± 0.978
0.0GlyXaa: 0.0 ± 0.0
His
1.192HisAla: 1.192 ± 0.474
0.0HisCys: 0.0 ± 0.0
0.596HisAsp: 0.596 ± 0.303
1.39HisGlu: 1.39 ± 0.581
0.397HisPhe: 0.397 ± 0.282
0.794HisGly: 0.794 ± 0.419
0.199HisHis: 0.199 ± 0.181
0.397HisIle: 0.397 ± 0.283
1.589HisLys: 1.589 ± 0.591
0.794HisLeu: 0.794 ± 0.368
0.596HisMet: 0.596 ± 0.319
0.397HisAsn: 0.397 ± 0.231
0.794HisPro: 0.794 ± 0.395
0.0HisGln: 0.0 ± 0.0
0.993HisArg: 0.993 ± 0.548
0.993HisSer: 0.993 ± 0.38
0.0HisThr: 0.0 ± 0.0
0.199HisVal: 0.199 ± 0.184
0.199HisTrp: 0.199 ± 0.163
0.397HisTyr: 0.397 ± 0.225
0.0HisXaa: 0.0 ± 0.0
Ile
5.561IleAla: 5.561 ± 1.193
0.993IleCys: 0.993 ± 0.415
2.185IleAsp: 2.185 ± 0.58
3.178IleGlu: 3.178 ± 0.73
1.787IlePhe: 1.787 ± 0.594
3.972IleGly: 3.972 ± 0.705
0.993IleHis: 0.993 ± 0.503
4.369IleIle: 4.369 ± 1.104
1.39IleLys: 1.39 ± 0.586
5.561IleLeu: 5.561 ± 1.318
2.582IleMet: 2.582 ± 0.683
2.979IleAsn: 2.979 ± 0.572
1.192IlePro: 1.192 ± 0.365
1.986IleGln: 1.986 ± 0.842
2.979IleArg: 2.979 ± 0.924
5.164IleSer: 5.164 ± 1.078
4.568IleThr: 4.568 ± 1.054
7.349IleVal: 7.349 ± 1.319
0.397IleTrp: 0.397 ± 0.236
1.986IleTyr: 1.986 ± 0.501
0.0IleXaa: 0.0 ± 0.0
Lys
3.575LysAla: 3.575 ± 1.113
0.0LysCys: 0.0 ± 0.0
1.986LysAsp: 1.986 ± 0.645
2.582LysGlu: 2.582 ± 0.756
1.589LysPhe: 1.589 ± 0.767
2.582LysGly: 2.582 ± 0.8
0.596LysHis: 0.596 ± 0.395
4.568LysIle: 4.568 ± 1.002
3.972LysLys: 3.972 ± 1.821
3.178LysLeu: 3.178 ± 0.708
2.383LysMet: 2.383 ± 0.688
3.575LysAsn: 3.575 ± 0.971
1.39LysPro: 1.39 ± 0.669
0.993LysGln: 0.993 ± 0.386
2.781LysArg: 2.781 ± 0.92
4.171LysSer: 4.171 ± 0.937
2.383LysThr: 2.383 ± 0.698
4.767LysVal: 4.767 ± 0.731
0.397LysTrp: 0.397 ± 0.293
1.986LysTyr: 1.986 ± 0.558
0.0LysXaa: 0.0 ± 0.0
Leu
8.54LeuAla: 8.54 ± 1.358
0.596LeuCys: 0.596 ± 0.424
3.972LeuAsp: 3.972 ± 0.874
5.561LeuGlu: 5.561 ± 1.208
2.383LeuPhe: 2.383 ± 0.775
5.164LeuGly: 5.164 ± 1.03
0.397LeuHis: 0.397 ± 0.348
3.575LeuIle: 3.575 ± 0.862
4.965LeuLys: 4.965 ± 1.156
5.362LeuLeu: 5.362 ± 1.235
2.582LeuMet: 2.582 ± 0.75
4.767LeuAsn: 4.767 ± 1.13
3.972LeuPro: 3.972 ± 0.84
1.589LeuGln: 1.589 ± 0.564
3.774LeuArg: 3.774 ± 0.828
6.157LeuSer: 6.157 ± 1.152
5.362LeuThr: 5.362 ± 0.921
4.369LeuVal: 4.369 ± 0.924
0.596LeuTrp: 0.596 ± 0.37
2.383LeuTyr: 2.383 ± 0.595
0.0LeuXaa: 0.0 ± 0.0
Met
4.568MetAla: 4.568 ± 0.883
0.0MetCys: 0.0 ± 0.0
1.39MetAsp: 1.39 ± 0.345
1.589MetGlu: 1.589 ± 0.57
1.39MetPhe: 1.39 ± 0.443
2.781MetGly: 2.781 ± 0.634
0.397MetHis: 0.397 ± 0.225
1.986MetIle: 1.986 ± 0.595
2.383MetLys: 2.383 ± 0.738
1.39MetLeu: 1.39 ± 0.52
0.993MetMet: 0.993 ± 0.385
1.39MetAsn: 1.39 ± 0.5
0.596MetPro: 0.596 ± 0.302
1.39MetGln: 1.39 ± 0.511
1.787MetArg: 1.787 ± 0.596
1.589MetSer: 1.589 ± 0.416
2.383MetThr: 2.383 ± 0.708
2.781MetVal: 2.781 ± 0.811
0.397MetTrp: 0.397 ± 0.238
1.192MetTyr: 1.192 ± 0.591
0.0MetXaa: 0.0 ± 0.0
Asn
4.568AsnAla: 4.568 ± 0.975
1.192AsnCys: 1.192 ± 0.487
1.986AsnAsp: 1.986 ± 0.63
1.39AsnGlu: 1.39 ± 0.571
1.39AsnPhe: 1.39 ± 0.515
5.164AsnGly: 5.164 ± 1.426
0.199AsnHis: 0.199 ± 0.201
1.192AsnIle: 1.192 ± 0.434
0.993AsnLys: 0.993 ± 0.503
3.376AsnLeu: 3.376 ± 0.959
1.192AsnMet: 1.192 ± 0.467
2.185AsnAsn: 2.185 ± 1.05
2.582AsnPro: 2.582 ± 0.641
1.986AsnGln: 1.986 ± 0.661
1.787AsnArg: 1.787 ± 0.533
4.369AsnSer: 4.369 ± 1.055
3.178AsnThr: 3.178 ± 0.655
4.369AsnVal: 4.369 ± 1.115
0.993AsnTrp: 0.993 ± 0.432
1.589AsnTyr: 1.589 ± 0.666
0.0AsnXaa: 0.0 ± 0.0
Pro
3.774ProAla: 3.774 ± 0.975
0.199ProCys: 0.199 ± 0.223
2.185ProAsp: 2.185 ± 0.661
2.185ProGlu: 2.185 ± 0.772
1.39ProPhe: 1.39 ± 0.7
2.979ProGly: 2.979 ± 0.705
0.596ProHis: 0.596 ± 0.291
2.383ProIle: 2.383 ± 0.651
2.185ProLys: 2.185 ± 0.734
2.383ProLeu: 2.383 ± 0.699
0.993ProMet: 0.993 ± 0.439
0.794ProAsn: 0.794 ± 0.459
0.993ProPro: 0.993 ± 0.355
0.993ProGln: 0.993 ± 0.333
1.787ProArg: 1.787 ± 0.494
3.178ProSer: 3.178 ± 0.863
3.178ProThr: 3.178 ± 1.033
5.362ProVal: 5.362 ± 0.81
0.397ProTrp: 0.397 ± 0.249
0.596ProTyr: 0.596 ± 0.347
0.0ProXaa: 0.0 ± 0.0
Gln
3.575GlnAla: 3.575 ± 0.725
0.199GlnCys: 0.199 ± 0.213
1.787GlnAsp: 1.787 ± 0.543
2.185GlnGlu: 2.185 ± 0.813
1.589GlnPhe: 1.589 ± 0.461
2.185GlnGly: 2.185 ± 0.681
0.0GlnHis: 0.0 ± 0.0
2.185GlnIle: 2.185 ± 0.701
1.39GlnLys: 1.39 ± 0.454
3.575GlnLeu: 3.575 ± 0.917
0.596GlnMet: 0.596 ± 0.481
0.596GlnAsn: 0.596 ± 0.319
1.39GlnPro: 1.39 ± 0.588
0.596GlnGln: 0.596 ± 0.422
1.589GlnArg: 1.589 ± 0.577
3.376GlnSer: 3.376 ± 0.845
2.383GlnThr: 2.383 ± 0.613
1.787GlnVal: 1.787 ± 0.605
0.596GlnTrp: 0.596 ± 0.305
1.986GlnTyr: 1.986 ± 0.524
0.0GlnXaa: 0.0 ± 0.0
Arg
4.369ArgAla: 4.369 ± 0.804
0.596ArgCys: 0.596 ± 0.363
2.781ArgAsp: 2.781 ± 0.636
2.582ArgGlu: 2.582 ± 0.79
1.589ArgPhe: 1.589 ± 0.456
2.781ArgGly: 2.781 ± 0.757
0.993ArgHis: 0.993 ± 0.372
3.376ArgIle: 3.376 ± 0.713
3.575ArgLys: 3.575 ± 1.259
4.965ArgLeu: 4.965 ± 1.102
1.787ArgMet: 1.787 ± 0.495
1.986ArgAsn: 1.986 ± 0.675
2.979ArgPro: 2.979 ± 0.649
1.589ArgGln: 1.589 ± 0.391
2.979ArgArg: 2.979 ± 1.055
2.582ArgSer: 2.582 ± 1.084
1.986ArgThr: 1.986 ± 0.674
2.781ArgVal: 2.781 ± 0.595
1.192ArgTrp: 1.192 ± 0.469
1.192ArgTyr: 1.192 ± 0.615
0.0ArgXaa: 0.0 ± 0.0
Ser
8.739SerAla: 8.739 ± 1.485
0.397SerCys: 0.397 ± 0.228
3.972SerAsp: 3.972 ± 1.027
4.767SerGlu: 4.767 ± 1.092
2.383SerPhe: 2.383 ± 0.794
8.739SerGly: 8.739 ± 1.803
0.0SerHis: 0.0 ± 0.0
3.376SerIle: 3.376 ± 0.53
2.979SerLys: 2.979 ± 0.612
4.767SerLeu: 4.767 ± 0.784
1.787SerMet: 1.787 ± 0.543
3.178SerAsn: 3.178 ± 0.776
2.383SerPro: 2.383 ± 0.608
3.774SerGln: 3.774 ± 0.848
3.376SerArg: 3.376 ± 0.899
5.561SerSer: 5.561 ± 1.424
4.965SerThr: 4.965 ± 0.93
5.362SerVal: 5.362 ± 0.832
0.397SerTrp: 0.397 ± 0.247
2.582SerTyr: 2.582 ± 0.792
0.0SerXaa: 0.0 ± 0.0
Thr
7.15ThrAla: 7.15 ± 1.103
0.794ThrCys: 0.794 ± 0.541
4.965ThrAsp: 4.965 ± 1.202
1.39ThrGlu: 1.39 ± 0.567
1.986ThrPhe: 1.986 ± 0.712
5.561ThrGly: 5.561 ± 1.154
0.794ThrHis: 0.794 ± 0.437
5.362ThrIle: 5.362 ± 0.917
2.979ThrLys: 2.979 ± 0.639
5.76ThrLeu: 5.76 ± 1.236
1.787ThrMet: 1.787 ± 0.421
1.787ThrAsn: 1.787 ± 0.594
2.979ThrPro: 2.979 ± 0.792
2.383ThrGln: 2.383 ± 0.647
3.376ThrArg: 3.376 ± 0.653
4.965ThrSer: 4.965 ± 1.225
5.561ThrThr: 5.561 ± 1.327
5.362ThrVal: 5.362 ± 1.237
0.993ThrTrp: 0.993 ± 0.444
2.582ThrTyr: 2.582 ± 0.663
0.0ThrXaa: 0.0 ± 0.0
Val
6.753ValAla: 6.753 ± 1.129
0.993ValCys: 0.993 ± 0.339
4.171ValAsp: 4.171 ± 0.933
3.575ValGlu: 3.575 ± 0.614
4.568ValPhe: 4.568 ± 1.045
5.958ValGly: 5.958 ± 1.08
0.397ValHis: 0.397 ± 0.281
5.164ValIle: 5.164 ± 0.957
2.582ValLys: 2.582 ± 0.646
5.76ValLeu: 5.76 ± 1.21
1.589ValMet: 1.589 ± 0.47
3.178ValAsn: 3.178 ± 0.62
3.178ValPro: 3.178 ± 0.587
2.383ValGln: 2.383 ± 0.569
3.575ValArg: 3.575 ± 0.992
4.965ValSer: 4.965 ± 0.827
6.356ValThr: 6.356 ± 1.167
4.965ValVal: 4.965 ± 1.379
1.39ValTrp: 1.39 ± 0.595
1.787ValTyr: 1.787 ± 0.585
0.0ValXaa: 0.0 ± 0.0
Trp
1.192TrpAla: 1.192 ± 0.458
0.0TrpCys: 0.0 ± 0.0
0.397TrpAsp: 0.397 ± 0.256
1.192TrpGlu: 1.192 ± 0.574
0.397TrpPhe: 0.397 ± 0.265
1.787TrpGly: 1.787 ± 0.517
0.199TrpHis: 0.199 ± 0.201
0.596TrpIle: 0.596 ± 0.308
0.993TrpLys: 0.993 ± 0.458
1.39TrpLeu: 1.39 ± 0.459
0.397TrpMet: 0.397 ± 0.192
0.397TrpAsn: 0.397 ± 0.278
0.397TrpPro: 0.397 ± 0.264
1.192TrpGln: 1.192 ± 0.54
0.794TrpArg: 0.794 ± 0.617
0.397TrpSer: 0.397 ± 0.235
0.397TrpThr: 0.397 ± 0.33
0.596TrpVal: 0.596 ± 0.329
0.596TrpTrp: 0.596 ± 0.264
0.199TrpTyr: 0.199 ± 0.163
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.582TyrAla: 2.582 ± 0.459
0.397TyrCys: 0.397 ± 0.293
2.185TyrAsp: 2.185 ± 0.48
1.39TyrGlu: 1.39 ± 0.543
1.589TyrPhe: 1.589 ± 0.707
2.979TyrGly: 2.979 ± 0.927
0.993TyrHis: 0.993 ± 0.356
0.993TyrIle: 0.993 ± 0.367
0.596TyrLys: 0.596 ± 0.384
2.383TyrLeu: 2.383 ± 0.514
0.397TyrMet: 0.397 ± 0.22
2.979TyrAsn: 2.979 ± 1.309
1.986TyrPro: 1.986 ± 0.525
1.986TyrGln: 1.986 ± 0.822
0.794TyrArg: 0.794 ± 0.429
2.582TyrSer: 2.582 ± 0.682
1.39TyrThr: 1.39 ± 0.431
2.781TyrVal: 2.781 ± 0.752
0.0TyrTrp: 0.0 ± 0.0
0.794TyrTyr: 0.794 ± 0.296
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 36 proteins (5036 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski