Mycobacterium phage Fascinus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1JV13|A0A2P1JV13_9CAUD Uncharacterized protein OS=Mycobacterium phage Fascinus OX=2108118 GN=39 PE=4 SV=1
MM1 pKa = 7.96SEE3 pKa = 5.11EE4 pKa = 3.92MQQLQKK10 pKa = 10.88EE11 pKa = 4.36LFSTYY16 pKa = 9.63VFYY19 pKa = 11.05EE20 pKa = 4.21PADD23 pKa = 3.7EE24 pKa = 4.36HH25 pKa = 7.13QGPGWMGFGLNSICWGAWQGWVGNLGGYY53 pKa = 9.1IAVIFYY59 pKa = 10.54NSEE62 pKa = 4.22DD63 pKa = 3.66GSVIAVTLNNFSAHH77 pKa = 6.4ADD79 pKa = 3.56AVDD82 pKa = 4.16LFYY85 pKa = 11.13QIAYY89 pKa = 9.85LLNPEE94 pKa = 4.45STGHH98 pKa = 6.67RR99 pKa = 11.84DD100 pKa = 2.94WIFRR104 pKa = 11.84PDD106 pKa = 3.47PAEE109 pKa = 4.9DD110 pKa = 3.62EE111 pKa = 5.0DD112 pKa = 4.22EE113 pKa = 4.53VRR115 pKa = 11.84DD116 pKa = 3.63PTLYY120 pKa = 9.89LTVEE124 pKa = 4.37STGDD128 pKa = 3.45SQIPADD134 pKa = 3.9VPFEE138 pKa = 3.98II139 pKa = 5.71

Molecular weight: 15.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1JUY8|A0A2P1JUY8_9CAUD Lysin B OS=Mycobacterium phage Fascinus OX=2108118 GN=9 PE=4 SV=1
MM1 pKa = 7.23GWEE4 pKa = 3.91SSDD7 pKa = 3.38RR8 pKa = 11.84RR9 pKa = 11.84GRR11 pKa = 11.84LPADD15 pKa = 3.3WPRR18 pKa = 11.84IRR20 pKa = 11.84RR21 pKa = 11.84EE22 pKa = 3.77VLRR25 pKa = 11.84AAGHH29 pKa = 6.4RR30 pKa = 11.84CQIRR34 pKa = 11.84YY35 pKa = 9.91ADD37 pKa = 3.88ICTGMATEE45 pKa = 4.27VDD47 pKa = 3.34HH48 pKa = 7.08VRR50 pKa = 11.84YY51 pKa = 9.51RR52 pKa = 11.84DD53 pKa = 3.58EE54 pKa = 4.33EE55 pKa = 4.6SPTRR59 pKa = 11.84AACKK63 pKa = 9.61ACHH66 pKa = 5.71ARR68 pKa = 11.84KK69 pKa = 9.55SAMEE73 pKa = 4.09GVAQRR78 pKa = 11.84AKK80 pKa = 10.29LRR82 pKa = 11.84AMKK85 pKa = 9.94KK86 pKa = 9.63RR87 pKa = 11.84PPPRR91 pKa = 11.84HH92 pKa = 5.87PGRR95 pKa = 11.84RR96 pKa = 11.84SNN98 pKa = 3.52

Molecular weight: 11.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

16155

43

859

187.8

20.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.028 ± 0.412

0.644 ± 0.105

6.605 ± 0.227

6.648 ± 0.298

3.132 ± 0.158

8.251 ± 0.42

2.024 ± 0.161

4.748 ± 0.221

4.104 ± 0.228

8.747 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.012 ± 0.112

3.262 ± 0.162

5.577 ± 0.22

3.312 ± 0.172

6.592 ± 0.366

5.769 ± 0.263

6.4 ± 0.296

7.298 ± 0.217

1.987 ± 0.104

2.86 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski