Amino acid dipepetide frequency for Mycobacterium phage Fascinus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.938AlaAla: 12.938 ± 1.14
0.929AlaCys: 0.929 ± 0.225
6.748AlaAsp: 6.748 ± 0.666
6.5AlaGlu: 6.5 ± 0.692
3.095AlaPhe: 3.095 ± 0.453
7.305AlaGly: 7.305 ± 0.756
1.486AlaHis: 1.486 ± 0.297
4.705AlaIle: 4.705 ± 0.573
4.086AlaLys: 4.086 ± 0.574
8.728AlaLeu: 8.728 ± 0.952
2.6AlaMet: 2.6 ± 0.552
2.786AlaAsn: 2.786 ± 0.437
4.767AlaPro: 4.767 ± 0.599
3.281AlaGln: 3.281 ± 0.513
6.067AlaArg: 6.067 ± 0.659
4.952AlaSer: 4.952 ± 0.547
5.943AlaThr: 5.943 ± 0.633
8.79AlaVal: 8.79 ± 0.718
1.919AlaTrp: 1.919 ± 0.337
2.662AlaTyr: 2.662 ± 0.35
0.0AlaXaa: 0.0 ± 0.0
Cys
0.619CysAla: 0.619 ± 0.216
0.062CysCys: 0.062 ± 0.056
0.557CysAsp: 0.557 ± 0.188
0.743CysGlu: 0.743 ± 0.238
0.186CysPhe: 0.186 ± 0.106
0.557CysGly: 0.557 ± 0.24
0.124CysHis: 0.124 ± 0.085
0.371CysIle: 0.371 ± 0.14
0.248CysLys: 0.248 ± 0.118
0.371CysLeu: 0.371 ± 0.179
0.186CysMet: 0.186 ± 0.085
0.186CysAsn: 0.186 ± 0.096
0.371CysPro: 0.371 ± 0.178
0.248CysGln: 0.248 ± 0.121
0.433CysArg: 0.433 ± 0.147
0.248CysSer: 0.248 ± 0.129
0.371CysThr: 0.371 ± 0.153
0.31CysVal: 0.31 ± 0.132
0.124CysTrp: 0.124 ± 0.095
0.124CysTyr: 0.124 ± 0.088
0.0CysXaa: 0.0 ± 0.0
Asp
5.819AspAla: 5.819 ± 0.584
0.495AspCys: 0.495 ± 0.181
4.643AspAsp: 4.643 ± 0.555
3.838AspGlu: 3.838 ± 0.51
2.105AspPhe: 2.105 ± 0.413
5.819AspGly: 5.819 ± 0.61
1.362AspHis: 1.362 ± 0.3
2.909AspIle: 2.909 ± 0.468
2.662AspLys: 2.662 ± 0.521
6.995AspLeu: 6.995 ± 0.602
1.238AspMet: 1.238 ± 0.244
1.733AspAsn: 1.733 ± 0.308
5.014AspPro: 5.014 ± 0.54
1.486AspGln: 1.486 ± 0.328
4.024AspArg: 4.024 ± 0.451
3.033AspSer: 3.033 ± 0.393
4.086AspThr: 4.086 ± 0.405
4.952AspVal: 4.952 ± 0.653
1.857AspTrp: 1.857 ± 0.356
1.981AspTyr: 1.981 ± 0.374
0.0AspXaa: 0.0 ± 0.0
Glu
6.314GluAla: 6.314 ± 0.734
0.31GluCys: 0.31 ± 0.17
4.457GluAsp: 4.457 ± 0.558
5.324GluGlu: 5.324 ± 0.639
2.414GluPhe: 2.414 ± 0.395
3.59GluGly: 3.59 ± 0.507
1.486GluHis: 1.486 ± 0.287
3.157GluIle: 3.157 ± 0.5
2.662GluLys: 2.662 ± 0.392
6.933GluLeu: 6.933 ± 0.715
1.61GluMet: 1.61 ± 0.285
1.857GluAsn: 1.857 ± 0.411
2.786GluPro: 2.786 ± 0.446
2.724GluGln: 2.724 ± 0.384
3.776GluArg: 3.776 ± 0.549
3.281GluSer: 3.281 ± 0.476
3.962GluThr: 3.962 ± 0.534
5.757GluVal: 5.757 ± 0.604
1.671GluTrp: 1.671 ± 0.398
2.414GluTyr: 2.414 ± 0.493
0.0GluXaa: 0.0 ± 0.0
Phe
2.662PheAla: 2.662 ± 0.45
0.371PheCys: 0.371 ± 0.179
2.662PheAsp: 2.662 ± 0.352
2.105PheGlu: 2.105 ± 0.324
0.31PhePhe: 0.31 ± 0.128
3.838PheGly: 3.838 ± 0.553
0.681PheHis: 0.681 ± 0.262
1.238PheIle: 1.238 ± 0.235
1.114PheLys: 1.114 ± 0.272
2.662PheLeu: 2.662 ± 0.485
0.557PheMet: 0.557 ± 0.208
1.114PheAsn: 1.114 ± 0.249
1.795PhePro: 1.795 ± 0.291
0.867PheGln: 0.867 ± 0.261
1.671PheArg: 1.671 ± 0.292
1.981PheSer: 1.981 ± 0.382
2.043PheThr: 2.043 ± 0.346
1.981PheVal: 1.981 ± 0.453
0.557PheTrp: 0.557 ± 0.195
1.114PheTyr: 1.114 ± 0.283
0.0PheXaa: 0.0 ± 0.0
Gly
6.686GlyAla: 6.686 ± 0.826
0.681GlyCys: 0.681 ± 0.245
6.314GlyAsp: 6.314 ± 0.635
4.333GlyGlu: 4.333 ± 0.514
2.971GlyPhe: 2.971 ± 0.551
7.552GlyGly: 7.552 ± 1.111
1.795GlyHis: 1.795 ± 0.364
4.148GlyIle: 4.148 ± 0.661
3.281GlyLys: 3.281 ± 0.504
7.8GlyLeu: 7.8 ± 0.662
1.61GlyMet: 1.61 ± 0.341
3.095GlyAsn: 3.095 ± 0.446
4.209GlyPro: 4.209 ± 0.621
2.352GlyGln: 2.352 ± 0.333
4.89GlyArg: 4.89 ± 0.576
5.448GlySer: 5.448 ± 0.717
5.076GlyThr: 5.076 ± 0.631
5.633GlyVal: 5.633 ± 0.64
2.229GlyTrp: 2.229 ± 0.38
2.414GlyTyr: 2.414 ± 0.393
0.0GlyXaa: 0.0 ± 0.0
His
1.548HisAla: 1.548 ± 0.298
0.124HisCys: 0.124 ± 0.082
1.238HisAsp: 1.238 ± 0.23
1.424HisGlu: 1.424 ± 0.312
0.619HisPhe: 0.619 ± 0.153
1.733HisGly: 1.733 ± 0.341
0.681HisHis: 0.681 ± 0.244
0.805HisIle: 0.805 ± 0.221
0.929HisLys: 0.929 ± 0.295
1.733HisLeu: 1.733 ± 0.387
0.124HisMet: 0.124 ± 0.092
0.371HisAsn: 0.371 ± 0.15
1.362HisPro: 1.362 ± 0.306
1.114HisGln: 1.114 ± 0.277
1.733HisArg: 1.733 ± 0.359
0.681HisSer: 0.681 ± 0.177
1.114HisThr: 1.114 ± 0.254
1.548HisVal: 1.548 ± 0.344
0.619HisTrp: 0.619 ± 0.18
0.743HisTyr: 0.743 ± 0.221
0.0HisXaa: 0.0 ± 0.0
Ile
6.067IleAla: 6.067 ± 0.733
0.31IleCys: 0.31 ± 0.132
2.909IleAsp: 2.909 ± 0.356
3.405IleGlu: 3.405 ± 0.437
0.743IlePhe: 0.743 ± 0.19
4.086IleGly: 4.086 ± 0.457
0.805IleHis: 0.805 ± 0.209
1.671IleIle: 1.671 ± 0.245
1.919IleLys: 1.919 ± 0.37
3.467IleLeu: 3.467 ± 0.391
0.929IleMet: 0.929 ± 0.202
2.043IleAsn: 2.043 ± 0.379
3.095IlePro: 3.095 ± 0.441
1.548IleGln: 1.548 ± 0.301
3.343IleArg: 3.343 ± 0.451
2.909IleSer: 2.909 ± 0.409
3.467IleThr: 3.467 ± 0.465
2.786IleVal: 2.786 ± 0.525
0.619IleTrp: 0.619 ± 0.184
1.362IleTyr: 1.362 ± 0.274
0.0IleXaa: 0.0 ± 0.0
Lys
3.59LysAla: 3.59 ± 0.491
0.124LysCys: 0.124 ± 0.089
2.414LysAsp: 2.414 ± 0.407
2.6LysGlu: 2.6 ± 0.406
1.424LysPhe: 1.424 ± 0.278
2.476LysGly: 2.476 ± 0.409
1.052LysHis: 1.052 ± 0.256
2.352LysIle: 2.352 ± 0.453
2.105LysLys: 2.105 ± 0.476
3.59LysLeu: 3.59 ± 0.505
0.929LysMet: 0.929 ± 0.218
1.548LysAsn: 1.548 ± 0.275
2.538LysPro: 2.538 ± 0.406
1.857LysGln: 1.857 ± 0.493
2.971LysArg: 2.971 ± 0.501
2.6LysSer: 2.6 ± 0.378
2.29LysThr: 2.29 ± 0.351
2.909LysVal: 2.909 ± 0.394
0.681LysTrp: 0.681 ± 0.215
0.99LysTyr: 0.99 ± 0.263
0.0LysXaa: 0.0 ± 0.0
Leu
9.224LeuAla: 9.224 ± 0.846
0.248LeuCys: 0.248 ± 0.107
6.376LeuAsp: 6.376 ± 0.581
5.695LeuGlu: 5.695 ± 0.637
2.29LeuPhe: 2.29 ± 0.389
7.49LeuGly: 7.49 ± 0.716
1.671LeuHis: 1.671 ± 0.294
4.519LeuIle: 4.519 ± 0.521
3.962LeuLys: 3.962 ± 0.473
6.067LeuLeu: 6.067 ± 0.507
1.733LeuMet: 1.733 ± 0.303
2.848LeuAsn: 2.848 ± 0.472
5.386LeuPro: 5.386 ± 0.599
2.6LeuGln: 2.6 ± 0.415
6.995LeuArg: 6.995 ± 0.549
5.757LeuSer: 5.757 ± 0.522
6.129LeuThr: 6.129 ± 0.497
5.262LeuVal: 5.262 ± 0.637
1.114LeuTrp: 1.114 ± 0.298
2.105LeuTyr: 2.105 ± 0.397
0.0LeuXaa: 0.0 ± 0.0
Met
2.6MetAla: 2.6 ± 0.355
0.0MetCys: 0.0 ± 0.0
1.052MetAsp: 1.052 ± 0.267
1.486MetGlu: 1.486 ± 0.343
0.681MetPhe: 0.681 ± 0.185
1.362MetGly: 1.362 ± 0.265
0.248MetHis: 0.248 ± 0.12
0.619MetIle: 0.619 ± 0.214
1.238MetLys: 1.238 ± 0.302
1.176MetLeu: 1.176 ± 0.272
0.124MetMet: 0.124 ± 0.098
0.867MetAsn: 0.867 ± 0.204
1.052MetPro: 1.052 ± 0.273
0.433MetGln: 0.433 ± 0.159
1.238MetArg: 1.238 ± 0.287
1.733MetSer: 1.733 ± 0.312
2.29MetThr: 2.29 ± 0.343
1.176MetVal: 1.176 ± 0.27
0.371MetTrp: 0.371 ± 0.163
0.371MetTyr: 0.371 ± 0.148
0.0MetXaa: 0.0 ± 0.0
Asn
3.652AsnAla: 3.652 ± 0.563
0.124AsnCys: 0.124 ± 0.086
2.229AsnAsp: 2.229 ± 0.363
2.167AsnGlu: 2.167 ± 0.382
0.929AsnPhe: 0.929 ± 0.282
3.281AsnGly: 3.281 ± 0.521
0.681AsnHis: 0.681 ± 0.181
1.733AsnIle: 1.733 ± 0.325
0.619AsnLys: 0.619 ± 0.204
2.786AsnLeu: 2.786 ± 0.323
0.557AsnMet: 0.557 ± 0.166
0.867AsnAsn: 0.867 ± 0.26
2.6AsnPro: 2.6 ± 0.316
0.99AsnGln: 0.99 ± 0.226
1.919AsnArg: 1.919 ± 0.335
1.61AsnSer: 1.61 ± 0.419
1.857AsnThr: 1.857 ± 0.297
2.229AsnVal: 2.229 ± 0.397
0.743AsnTrp: 0.743 ± 0.198
1.052AsnTyr: 1.052 ± 0.266
0.0AsnXaa: 0.0 ± 0.0
Pro
5.448ProAla: 5.448 ± 0.498
0.371ProCys: 0.371 ± 0.138
4.209ProAsp: 4.209 ± 0.418
4.643ProGlu: 4.643 ± 0.46
2.229ProPhe: 2.229 ± 0.365
4.89ProGly: 4.89 ± 0.679
0.867ProHis: 0.867 ± 0.222
2.538ProIle: 2.538 ± 0.414
1.981ProLys: 1.981 ± 0.263
4.643ProLeu: 4.643 ± 0.563
0.929ProMet: 0.929 ± 0.201
1.548ProAsn: 1.548 ± 0.313
2.786ProPro: 2.786 ± 0.446
1.548ProGln: 1.548 ± 0.375
3.405ProArg: 3.405 ± 0.542
3.838ProSer: 3.838 ± 0.491
3.838ProThr: 3.838 ± 0.495
3.59ProVal: 3.59 ± 0.496
0.743ProTrp: 0.743 ± 0.286
1.733ProTyr: 1.733 ± 0.351
0.0ProXaa: 0.0 ± 0.0
Gln
3.405GlnAla: 3.405 ± 0.59
0.062GlnCys: 0.062 ± 0.063
1.424GlnAsp: 1.424 ± 0.368
1.548GlnGlu: 1.548 ± 0.283
1.052GlnPhe: 1.052 ± 0.208
2.538GlnGly: 2.538 ± 0.334
0.557GlnHis: 0.557 ± 0.166
2.352GlnIle: 2.352 ± 0.458
1.486GlnLys: 1.486 ± 0.301
4.148GlnLeu: 4.148 ± 0.593
0.929GlnMet: 0.929 ± 0.23
0.433GlnAsn: 0.433 ± 0.174
2.29GlnPro: 2.29 ± 0.38
1.857GlnGln: 1.857 ± 0.382
1.857GlnArg: 1.857 ± 0.381
1.671GlnSer: 1.671 ± 0.284
1.61GlnThr: 1.61 ± 0.317
2.414GlnVal: 2.414 ± 0.299
0.681GlnTrp: 0.681 ± 0.143
0.805GlnTyr: 0.805 ± 0.179
0.0GlnXaa: 0.0 ± 0.0
Arg
5.881ArgAla: 5.881 ± 0.698
0.805ArgCys: 0.805 ± 0.244
3.157ArgAsp: 3.157 ± 0.366
4.643ArgGlu: 4.643 ± 0.609
2.229ArgPhe: 2.229 ± 0.465
5.386ArgGly: 5.386 ± 0.685
1.3ArgHis: 1.3 ± 0.282
3.095ArgIle: 3.095 ± 0.428
3.343ArgLys: 3.343 ± 0.562
6.129ArgLeu: 6.129 ± 0.739
1.981ArgMet: 1.981 ± 0.374
2.414ArgAsn: 2.414 ± 0.472
2.538ArgPro: 2.538 ± 0.384
2.229ArgGln: 2.229 ± 0.341
5.509ArgArg: 5.509 ± 0.666
3.962ArgSer: 3.962 ± 0.516
3.281ArgThr: 3.281 ± 0.619
5.138ArgVal: 5.138 ± 0.563
1.176ArgTrp: 1.176 ± 0.272
1.733ArgTyr: 1.733 ± 0.297
0.0ArgXaa: 0.0 ± 0.0
Ser
6.067SerAla: 6.067 ± 0.669
0.433SerCys: 0.433 ± 0.195
3.405SerAsp: 3.405 ± 0.476
4.024SerGlu: 4.024 ± 0.507
1.857SerPhe: 1.857 ± 0.406
5.509SerGly: 5.509 ± 0.651
1.424SerHis: 1.424 ± 0.277
2.414SerIle: 2.414 ± 0.386
2.476SerLys: 2.476 ± 0.362
4.952SerLeu: 4.952 ± 0.55
1.3SerMet: 1.3 ± 0.306
1.981SerAsn: 1.981 ± 0.329
2.971SerPro: 2.971 ± 0.435
1.981SerGln: 1.981 ± 0.303
3.281SerArg: 3.281 ± 0.406
3.281SerSer: 3.281 ± 0.583
3.343SerThr: 3.343 ± 0.506
4.148SerVal: 4.148 ± 0.443
1.3SerTrp: 1.3 ± 0.331
1.548SerTyr: 1.548 ± 0.347
0.0SerXaa: 0.0 ± 0.0
Thr
6.252ThrAla: 6.252 ± 0.696
0.186ThrCys: 0.186 ± 0.11
3.9ThrAsp: 3.9 ± 0.646
4.148ThrGlu: 4.148 ± 0.536
2.229ThrPhe: 2.229 ± 0.418
6.438ThrGly: 6.438 ± 0.668
1.176ThrHis: 1.176 ± 0.313
2.476ThrIle: 2.476 ± 0.464
2.352ThrLys: 2.352 ± 0.343
5.448ThrLeu: 5.448 ± 0.64
0.929ThrMet: 0.929 ± 0.214
2.29ThrAsn: 2.29 ± 0.457
4.086ThrPro: 4.086 ± 0.501
1.919ThrGln: 1.919 ± 0.331
3.405ThrArg: 3.405 ± 0.575
3.467ThrSer: 3.467 ± 0.461
4.024ThrThr: 4.024 ± 0.595
6.19ThrVal: 6.19 ± 0.715
1.238ThrTrp: 1.238 ± 0.237
1.857ThrTyr: 1.857 ± 0.398
0.0ThrXaa: 0.0 ± 0.0
Val
7.243ValAla: 7.243 ± 0.832
0.31ValCys: 0.31 ± 0.135
5.386ValAsp: 5.386 ± 0.603
4.643ValGlu: 4.643 ± 0.495
2.352ValPhe: 2.352 ± 0.391
4.643ValGly: 4.643 ± 0.67
1.671ValHis: 1.671 ± 0.261
4.024ValIle: 4.024 ± 0.546
3.095ValLys: 3.095 ± 0.414
5.386ValLeu: 5.386 ± 0.546
1.052ValMet: 1.052 ± 0.282
2.6ValAsn: 2.6 ± 0.36
4.271ValPro: 4.271 ± 0.551
2.043ValGln: 2.043 ± 0.388
5.757ValArg: 5.757 ± 0.701
4.705ValSer: 4.705 ± 0.507
5.509ValThr: 5.509 ± 0.691
4.457ValVal: 4.457 ± 0.727
1.114ValTrp: 1.114 ± 0.245
2.662ValTyr: 2.662 ± 0.432
0.0ValXaa: 0.0 ± 0.0
Trp
1.733TrpAla: 1.733 ± 0.329
0.186TrpCys: 0.186 ± 0.107
1.548TrpAsp: 1.548 ± 0.3
0.99TrpGlu: 0.99 ± 0.206
0.929TrpPhe: 0.929 ± 0.254
1.486TrpGly: 1.486 ± 0.256
0.371TrpHis: 0.371 ± 0.146
0.805TrpIle: 0.805 ± 0.244
0.371TrpLys: 0.371 ± 0.208
1.857TrpLeu: 1.857 ± 0.236
0.31TrpMet: 0.31 ± 0.144
0.805TrpAsn: 0.805 ± 0.214
0.805TrpPro: 0.805 ± 0.262
1.052TrpGln: 1.052 ± 0.236
0.99TrpArg: 0.99 ± 0.273
1.052TrpSer: 1.052 ± 0.254
1.61TrpThr: 1.61 ± 0.405
1.919TrpVal: 1.919 ± 0.285
0.681TrpTrp: 0.681 ± 0.261
0.371TrpTyr: 0.371 ± 0.142
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.538TyrAla: 2.538 ± 0.429
0.31TyrCys: 0.31 ± 0.172
1.424TyrAsp: 1.424 ± 0.307
2.229TyrGlu: 2.229 ± 0.375
0.681TyrPhe: 0.681 ± 0.201
2.538TyrGly: 2.538 ± 0.451
0.805TyrHis: 0.805 ± 0.215
1.548TyrIle: 1.548 ± 0.365
1.114TyrLys: 1.114 ± 0.238
2.6TyrLeu: 2.6 ± 0.376
0.433TyrMet: 0.433 ± 0.125
1.238TyrAsn: 1.238 ± 0.266
1.052TyrPro: 1.052 ± 0.243
0.99TyrGln: 0.99 ± 0.253
2.662TyrArg: 2.662 ± 0.381
1.486TyrSer: 1.486 ± 0.237
2.167TyrThr: 2.167 ± 0.382
1.795TyrVal: 1.795 ± 0.313
0.433TyrTrp: 0.433 ± 0.19
0.557TyrTyr: 0.557 ± 0.161
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 86 proteins (16155 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski