Amino acid dipepetide frequency for Methylovirgula sp. 4M-Z18

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.27AlaAla: 17.27 ± 0.16
1.181AlaCys: 1.181 ± 0.029
6.741AlaAsp: 6.741 ± 0.071
6.796AlaGlu: 6.796 ± 0.088
4.625AlaPhe: 4.625 ± 0.058
10.092AlaGly: 10.092 ± 0.116
2.71AlaHis: 2.71 ± 0.042
6.606AlaIle: 6.606 ± 0.074
4.979AlaLys: 4.979 ± 0.075
13.585AlaLeu: 13.585 ± 0.11
3.397AlaMet: 3.397 ± 0.053
3.275AlaAsn: 3.275 ± 0.059
5.824AlaPro: 5.824 ± 0.073
4.773AlaGln: 4.773 ± 0.073
8.343AlaArg: 8.343 ± 0.106
6.626AlaSer: 6.626 ± 0.083
6.153AlaThr: 6.153 ± 0.065
8.341AlaVal: 8.341 ± 0.082
1.51AlaTrp: 1.51 ± 0.036
2.772AlaTyr: 2.772 ± 0.046
0.0AlaXaa: 0.0 ± 0.0
Cys
1.116CysAla: 1.116 ± 0.032
0.132CysCys: 0.132 ± 0.01
0.52CysAsp: 0.52 ± 0.018
0.432CysGlu: 0.432 ± 0.019
0.358CysPhe: 0.358 ± 0.014
1.019CysGly: 1.019 ± 0.03
0.229CysHis: 0.229 ± 0.012
0.463CysIle: 0.463 ± 0.019
0.244CysLys: 0.244 ± 0.013
0.89CysLeu: 0.89 ± 0.027
0.153CysMet: 0.153 ± 0.01
0.243CysAsn: 0.243 ± 0.014
0.436CysPro: 0.436 ± 0.018
0.234CysGln: 0.234 ± 0.014
0.606CysArg: 0.606 ± 0.021
0.48CysSer: 0.48 ± 0.018
0.439CysThr: 0.439 ± 0.019
0.716CysVal: 0.716 ± 0.022
0.126CysTrp: 0.126 ± 0.008
0.222CysTyr: 0.222 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
6.988AspAla: 6.988 ± 0.08
0.504AspCys: 0.504 ± 0.02
3.013AspAsp: 3.013 ± 0.051
3.192AspGlu: 3.192 ± 0.053
2.284AspPhe: 2.284 ± 0.041
4.796AspGly: 4.796 ± 0.07
1.359AspHis: 1.359 ± 0.03
3.129AspIle: 3.129 ± 0.053
1.908AspLys: 1.908 ± 0.043
6.193AspLeu: 6.193 ± 0.066
1.441AspMet: 1.441 ± 0.031
1.293AspAsn: 1.293 ± 0.032
3.309AspPro: 3.309 ± 0.049
1.767AspGln: 1.767 ± 0.04
3.661AspArg: 3.661 ± 0.053
2.129AspSer: 2.129 ± 0.037
2.54AspThr: 2.54 ± 0.048
4.322AspVal: 4.322 ± 0.058
0.933AspTrp: 0.933 ± 0.029
1.548AspTyr: 1.548 ± 0.033
0.0AspXaa: 0.0 ± 0.0
Glu
7.035GluAla: 7.035 ± 0.09
0.371GluCys: 0.371 ± 0.017
2.464GluAsp: 2.464 ± 0.052
2.646GluGlu: 2.646 ± 0.061
1.619GluPhe: 1.619 ± 0.031
3.619GluGly: 3.619 ± 0.065
1.218GluHis: 1.218 ± 0.031
3.407GluIle: 3.407 ± 0.054
2.136GluLys: 2.136 ± 0.044
4.823GluLeu: 4.823 ± 0.072
1.387GluMet: 1.387 ± 0.037
1.452GluAsn: 1.452 ± 0.032
2.587GluPro: 2.587 ± 0.049
1.894GluGln: 1.894 ± 0.041
4.315GluArg: 4.315 ± 0.068
2.271GluSer: 2.271 ± 0.04
3.245GluThr: 3.245 ± 0.044
3.336GluVal: 3.336 ± 0.049
0.624GluTrp: 0.624 ± 0.023
0.965GluTyr: 0.965 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
5.086PheAla: 5.086 ± 0.066
0.442PheCys: 0.442 ± 0.018
2.708PheAsp: 2.708 ± 0.044
1.988PheGlu: 1.988 ± 0.036
1.442PhePhe: 1.442 ± 0.033
3.785PheGly: 3.785 ± 0.053
0.859PheHis: 0.859 ± 0.025
1.736PheIle: 1.736 ± 0.033
1.125PheLys: 1.125 ± 0.026
3.56PheLeu: 3.56 ± 0.059
0.801PheMet: 0.801 ± 0.024
1.145PheAsn: 1.145 ± 0.028
1.551PhePro: 1.551 ± 0.034
1.073PheGln: 1.073 ± 0.026
2.166PheArg: 2.166 ± 0.043
2.269PheSer: 2.269 ± 0.046
2.048PheThr: 2.048 ± 0.041
2.999PheVal: 2.999 ± 0.047
0.592PheTrp: 0.592 ± 0.023
0.98PheTyr: 0.98 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
9.545GlyAla: 9.545 ± 0.121
0.86GlyCys: 0.86 ± 0.027
4.275GlyAsp: 4.275 ± 0.058
4.23GlyGlu: 4.23 ± 0.065
3.686GlyPhe: 3.686 ± 0.062
7.449GlyGly: 7.449 ± 0.434
1.982GlyHis: 1.982 ± 0.041
4.619GlyIle: 4.619 ± 0.069
3.464GlyLys: 3.464 ± 0.05
8.809GlyLeu: 8.809 ± 0.094
2.093GlyMet: 2.093 ± 0.044
2.386GlyAsn: 2.386 ± 0.11
3.416GlyPro: 3.416 ± 0.057
2.877GlyGln: 2.877 ± 0.05
5.552GlyArg: 5.552 ± 0.07
4.597GlySer: 4.597 ± 0.118
4.88GlyThr: 4.88 ± 0.117
6.096GlyVal: 6.096 ± 0.07
1.319GlyTrp: 1.319 ± 0.033
2.224GlyTyr: 2.224 ± 0.041
0.0GlyXaa: 0.0 ± 0.0
His
2.565HisAla: 2.565 ± 0.046
0.253HisCys: 0.253 ± 0.013
1.361HisAsp: 1.361 ± 0.034
1.168HisGlu: 1.168 ± 0.031
1.032HisPhe: 1.032 ± 0.026
2.087HisGly: 2.087 ± 0.041
0.669HisHis: 0.669 ± 0.024
1.177HisIle: 1.177 ± 0.029
0.626HisLys: 0.626 ± 0.02
2.387HisLeu: 2.387 ± 0.045
0.596HisMet: 0.596 ± 0.021
0.522HisAsn: 0.522 ± 0.021
1.373HisPro: 1.373 ± 0.037
0.664HisGln: 0.664 ± 0.022
1.49HisArg: 1.49 ± 0.036
0.995HisSer: 0.995 ± 0.029
0.906HisThr: 0.906 ± 0.025
1.812HisVal: 1.812 ± 0.036
0.38HisTrp: 0.38 ± 0.018
0.636HisTyr: 0.636 ± 0.024
0.0HisXaa: 0.0 ± 0.0
Ile
7.767IleAla: 7.767 ± 0.078
0.581IleCys: 0.581 ± 0.019
3.695IleAsp: 3.695 ± 0.052
3.264IleGlu: 3.264 ± 0.044
2.0IlePhe: 2.0 ± 0.04
5.305IleGly: 5.305 ± 0.073
1.066IleHis: 1.066 ± 0.03
2.468IleIle: 2.468 ± 0.051
1.642IleLys: 1.642 ± 0.038
4.845IleLeu: 4.845 ± 0.066
1.028IleMet: 1.028 ± 0.029
1.499IleAsn: 1.499 ± 0.034
2.373IlePro: 2.373 ± 0.04
1.259IleGln: 1.259 ± 0.027
3.176IleArg: 3.176 ± 0.048
2.973IleSer: 2.973 ± 0.05
2.712IleThr: 2.712 ± 0.042
4.611IleVal: 4.611 ± 0.057
0.679IleTrp: 0.679 ± 0.025
1.212IleTyr: 1.212 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
4.512LysAla: 4.512 ± 0.065
0.202LysCys: 0.202 ± 0.012
1.926LysAsp: 1.926 ± 0.038
1.61LysGlu: 1.61 ± 0.042
1.055LysPhe: 1.055 ± 0.032
2.822LysGly: 2.822 ± 0.052
0.731LysHis: 0.731 ± 0.024
2.21LysIle: 2.21 ± 0.042
1.432LysLys: 1.432 ± 0.044
3.659LysLeu: 3.659 ± 0.05
0.911LysMet: 0.911 ± 0.026
0.978LysAsn: 0.978 ± 0.028
2.193LysPro: 2.193 ± 0.044
1.143LysGln: 1.143 ± 0.031
2.387LysArg: 2.387 ± 0.046
2.1LysSer: 2.1 ± 0.041
2.041LysThr: 2.041 ± 0.037
2.659LysVal: 2.659 ± 0.048
0.386LysTrp: 0.386 ± 0.016
0.728LysTyr: 0.728 ± 0.025
0.0LysXaa: 0.0 ± 0.0
Leu
13.751LeuAla: 13.751 ± 0.108
1.003LeuCys: 1.003 ± 0.03
6.003LeuAsp: 6.003 ± 0.081
4.769LeuGlu: 4.769 ± 0.063
3.677LeuPhe: 3.677 ± 0.061
8.25LeuGly: 8.25 ± 0.098
2.077LeuHis: 2.077 ± 0.044
5.322LeuIle: 5.322 ± 0.057
3.815LeuLys: 3.815 ± 0.059
9.271LeuLeu: 9.271 ± 0.093
2.298LeuMet: 2.298 ± 0.044
2.898LeuAsn: 2.898 ± 0.052
5.564LeuPro: 5.564 ± 0.078
3.205LeuGln: 3.205 ± 0.045
6.647LeuArg: 6.647 ± 0.083
6.338LeuSer: 6.338 ± 0.064
5.745LeuThr: 5.745 ± 0.075
7.281LeuVal: 7.281 ± 0.088
1.186LeuTrp: 1.186 ± 0.033
2.106LeuTyr: 2.106 ± 0.038
0.0LeuXaa: 0.0 ± 0.0
Met
2.894MetAla: 2.894 ± 0.045
0.181MetCys: 0.181 ± 0.012
1.1MetAsp: 1.1 ± 0.028
0.984MetGlu: 0.984 ± 0.029
0.721MetPhe: 0.721 ± 0.025
1.738MetGly: 1.738 ± 0.039
0.481MetHis: 0.481 ± 0.019
1.414MetIle: 1.414 ± 0.034
0.964MetLys: 0.964 ± 0.029
2.418MetLeu: 2.418 ± 0.046
0.665MetMet: 0.665 ± 0.023
0.782MetAsn: 0.782 ± 0.025
1.508MetPro: 1.508 ± 0.03
0.917MetGln: 0.917 ± 0.025
1.856MetArg: 1.856 ± 0.034
1.734MetSer: 1.734 ± 0.035
1.794MetThr: 1.794 ± 0.035
1.593MetVal: 1.593 ± 0.032
0.225MetTrp: 0.225 ± 0.012
0.331MetTyr: 0.331 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.438AsnAla: 3.438 ± 0.058
0.268AsnCys: 0.268 ± 0.013
1.532AsnAsp: 1.532 ± 0.041
1.28AsnGlu: 1.28 ± 0.031
1.093AsnPhe: 1.093 ± 0.027
2.741AsnGly: 2.741 ± 0.101
0.576AsnHis: 0.576 ± 0.021
1.523AsnIle: 1.523 ± 0.031
0.782AsnLys: 0.782 ± 0.027
2.779AsnLeu: 2.779 ± 0.048
0.648AsnMet: 0.648 ± 0.023
0.827AsnAsn: 0.827 ± 0.038
1.819AsnPro: 1.819 ± 0.036
0.841AsnGln: 0.841 ± 0.028
1.639AsnArg: 1.639 ± 0.031
1.425AsnSer: 1.425 ± 0.051
1.308AsnThr: 1.308 ± 0.045
2.316AsnVal: 2.316 ± 0.05
0.459AsnTrp: 0.459 ± 0.017
0.721AsnTyr: 0.721 ± 0.024
0.0AsnXaa: 0.0 ± 0.0
Pro
6.022ProAla: 6.022 ± 0.082
0.344ProCys: 0.344 ± 0.015
3.51ProAsp: 3.51 ± 0.048
3.136ProGlu: 3.136 ± 0.055
1.964ProPhe: 1.964 ± 0.039
4.295ProGly: 4.295 ± 0.057
1.301ProHis: 1.301 ± 0.032
2.506ProIle: 2.506 ± 0.044
1.864ProLys: 1.864 ± 0.032
4.821ProLeu: 4.821 ± 0.065
1.197ProMet: 1.197 ± 0.028
1.593ProAsn: 1.593 ± 0.037
2.672ProPro: 2.672 ± 0.061
2.009ProGln: 2.009 ± 0.046
2.81ProArg: 2.81 ± 0.05
2.837ProSer: 2.837 ± 0.052
2.542ProThr: 2.542 ± 0.048
4.001ProVal: 4.001 ± 0.064
0.696ProTrp: 0.696 ± 0.024
1.256ProTyr: 1.256 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
4.34GlnAla: 4.34 ± 0.06
0.24GlnCys: 0.24 ± 0.013
1.703GlnAsp: 1.703 ± 0.035
1.583GlnGlu: 1.583 ± 0.036
1.148GlnPhe: 1.148 ± 0.028
2.542GlnGly: 2.542 ± 0.041
0.764GlnHis: 0.764 ± 0.023
2.064GlnIle: 2.064 ± 0.043
1.295GlnLys: 1.295 ± 0.034
2.908GlnLeu: 2.908 ± 0.051
0.946GlnMet: 0.946 ± 0.024
1.071GlnAsn: 1.071 ± 0.034
1.768GlnPro: 1.768 ± 0.04
1.39GlnGln: 1.39 ± 0.04
2.433GlnArg: 2.433 ± 0.046
2.047GlnSer: 2.047 ± 0.066
1.836GlnThr: 1.836 ± 0.041
2.294GlnVal: 2.294 ± 0.044
0.434GlnTrp: 0.434 ± 0.018
0.656GlnTyr: 0.656 ± 0.021
0.0GlnXaa: 0.0 ± 0.0
Arg
7.698ArgAla: 7.698 ± 0.088
0.495ArgCys: 0.495 ± 0.02
3.824ArgAsp: 3.824 ± 0.056
3.702ArgGlu: 3.702 ± 0.06
2.781ArgPhe: 2.781 ± 0.041
4.534ArgGly: 4.534 ± 0.067
1.724ArgHis: 1.724 ± 0.039
3.801ArgIle: 3.801 ± 0.051
2.26ArgLys: 2.26 ± 0.046
7.391ArgLeu: 7.391 ± 0.09
1.677ArgMet: 1.677 ± 0.035
1.842ArgAsn: 1.842 ± 0.036
3.251ArgPro: 3.251 ± 0.054
2.52ArgGln: 2.52 ± 0.043
5.069ArgArg: 5.069 ± 0.081
3.363ArgSer: 3.363 ± 0.068
3.072ArgThr: 3.072 ± 0.051
4.577ArgVal: 4.577 ± 0.063
0.885ArgTrp: 0.885 ± 0.026
1.619ArgTyr: 1.619 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
6.462SerAla: 6.462 ± 0.076
0.441SerCys: 0.441 ± 0.016
3.052SerAsp: 3.052 ± 0.053
2.599SerGlu: 2.599 ± 0.048
2.445SerPhe: 2.445 ± 0.04
5.634SerGly: 5.634 ± 0.129
1.258SerHis: 1.258 ± 0.032
2.873SerIle: 2.873 ± 0.051
1.683SerLys: 1.683 ± 0.035
5.579SerLeu: 5.579 ± 0.065
1.218SerMet: 1.218 ± 0.031
1.531SerAsn: 1.531 ± 0.045
2.855SerPro: 2.855 ± 0.045
1.675SerGln: 1.675 ± 0.031
3.223SerArg: 3.223 ± 0.058
3.158SerSer: 3.158 ± 0.09
2.738SerThr: 2.738 ± 0.051
4.098SerVal: 4.098 ± 0.079
0.766SerTrp: 0.766 ± 0.026
1.355SerTyr: 1.355 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
5.985ThrAla: 5.985 ± 0.085
0.506ThrCys: 0.506 ± 0.017
2.73ThrAsp: 2.73 ± 0.041
2.346ThrGlu: 2.346 ± 0.045
2.169ThrPhe: 2.169 ± 0.039
4.998ThrGly: 4.998 ± 0.091
1.182ThrHis: 1.182 ± 0.028
3.194ThrIle: 3.194 ± 0.058
1.745ThrLys: 1.745 ± 0.036
5.915ThrLeu: 5.915 ± 0.073
1.238ThrMet: 1.238 ± 0.03
1.542ThrAsn: 1.542 ± 0.048
3.249ThrPro: 3.249 ± 0.049
1.646ThrGln: 1.646 ± 0.035
3.08ThrArg: 3.08 ± 0.05
3.009ThrSer: 3.009 ± 0.048
2.81ThrThr: 2.81 ± 0.055
4.049ThrVal: 4.049 ± 0.063
0.694ThrTrp: 0.694 ± 0.027
1.367ThrTyr: 1.367 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
9.219ValAla: 9.219 ± 0.084
0.651ValCys: 0.651 ± 0.025
4.047ValAsp: 4.047 ± 0.057
4.034ValGlu: 4.034 ± 0.065
2.727ValPhe: 2.727 ± 0.053
5.619ValGly: 5.619 ± 0.106
1.585ValHis: 1.585 ± 0.032
3.961ValIle: 3.961 ± 0.061
2.584ValLys: 2.584 ± 0.048
7.318ValLeu: 7.318 ± 0.082
1.768ValMet: 1.768 ± 0.037
2.038ValAsn: 2.038 ± 0.047
3.709ValPro: 3.709 ± 0.052
2.253ValGln: 2.253 ± 0.038
4.749ValArg: 4.749 ± 0.065
4.267ValSer: 4.267 ± 0.055
4.735ValThr: 4.735 ± 0.071
5.668ValVal: 5.668 ± 0.067
0.894ValTrp: 0.894 ± 0.026
1.481ValTyr: 1.481 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.256TrpAla: 1.256 ± 0.03
0.138TrpCys: 0.138 ± 0.011
0.625TrpAsp: 0.625 ± 0.022
0.487TrpGlu: 0.487 ± 0.021
0.528TrpPhe: 0.528 ± 0.021
0.924TrpGly: 0.924 ± 0.025
0.383TrpHis: 0.383 ± 0.017
0.713TrpIle: 0.713 ± 0.023
0.447TrpLys: 0.447 ± 0.018
1.657TrpLeu: 1.657 ± 0.042
0.346TrpMet: 0.346 ± 0.016
0.455TrpAsn: 0.455 ± 0.019
0.744TrpPro: 0.744 ± 0.024
0.603TrpGln: 0.603 ± 0.02
1.216TrpArg: 1.216 ± 0.035
0.824TrpSer: 0.824 ± 0.028
0.772TrpThr: 0.772 ± 0.026
0.782TrpVal: 0.782 ± 0.022
0.22TrpTrp: 0.22 ± 0.013
0.284TrpTyr: 0.284 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.651TyrAla: 2.651 ± 0.04
0.263TyrCys: 0.263 ± 0.014
1.48TyrAsp: 1.48 ± 0.032
1.17TyrGlu: 1.17 ± 0.03
1.003TyrPhe: 1.003 ± 0.028
2.109TyrGly: 2.109 ± 0.042
0.547TyrHis: 0.547 ± 0.02
0.969TyrIle: 0.969 ± 0.027
0.69TyrLys: 0.69 ± 0.025
2.396TyrLeu: 2.396 ± 0.042
0.49TyrMet: 0.49 ± 0.021
0.644TyrAsn: 0.644 ± 0.024
1.176TyrPro: 1.176 ± 0.032
0.716TyrGln: 0.716 ± 0.024
1.668TyrArg: 1.668 ± 0.036
1.201TyrSer: 1.201 ± 0.03
1.142TyrThr: 1.142 ± 0.031
1.756TyrVal: 1.756 ± 0.041
0.393TyrTrp: 0.393 ± 0.018
0.64TyrTyr: 0.64 ± 0.025
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4535 proteins (1443161 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski