Oceanomicrobium pacificus
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3093 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6B0TXR5|A0A6B0TXR5_9RHOB Uncharacterized protein OS=Oceanomicrobium pacificus OX=2692916 GN=GSH16_13670 PE=4 SV=1
MM1 pKa = 7.37 KK2 pKa = 10.37 KK3 pKa = 10.28 AVALVLASSVSAGAAYY19 pKa = 9.76 AGNMNFEE26 pKa = 4.39 EE27 pKa = 4.5 PVEE30 pKa = 4.17 PVVVAPVAVVDD41 pKa = 4.37 TGYY44 pKa = 10.97 DD45 pKa = 2.81 WSGFYY50 pKa = 10.82 GGAQLGYY57 pKa = 10.97 GRR59 pKa = 11.84 GDD61 pKa = 3.09 VDD63 pKa = 4.24 GVGDD67 pKa = 3.7 TEE69 pKa = 4.9 GYY71 pKa = 10.28 LGGVHH76 pKa = 7.17 AGYY79 pKa = 10.84 NYY81 pKa = 10.97 DD82 pKa = 3.31 FGNWVIGGEE91 pKa = 4.57 LDD93 pKa = 4.09 VDD95 pKa = 3.72 WADD98 pKa = 4.52 LEE100 pKa = 4.36 VDD102 pKa = 3.67 NTDD105 pKa = 3.03 ATIDD109 pKa = 4.06 GIARR113 pKa = 11.84 LKK115 pKa = 11.09 LKK117 pKa = 10.55 GGYY120 pKa = 10.06 DD121 pKa = 3.87 FGRR124 pKa = 11.84 TLVYY128 pKa = 9.57 GTVGAAAANLSSDD141 pKa = 3.36 TLGDD145 pKa = 3.66 DD146 pKa = 3.5 RR147 pKa = 11.84 GNGYY151 pKa = 9.67 VAGLGVDD158 pKa = 3.77 YY159 pKa = 10.92 AVTDD163 pKa = 3.3 NWIVGAEE170 pKa = 3.94 YY171 pKa = 10.17 LYY173 pKa = 10.93 HH174 pKa = 7.37 DD175 pKa = 4.48 FKK177 pKa = 11.52 DD178 pKa = 3.95 YY179 pKa = 11.71 GDD181 pKa = 4.87 LGTDD185 pKa = 3.43 VNVNTLKK192 pKa = 11.1 AKK194 pKa = 10.28 VSYY197 pKa = 10.77 KK198 pKa = 10.35 FF199 pKa = 3.37
Molecular weight: 20.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.777
IPC2_protein 3.961
IPC_protein 3.961
Toseland 3.732
ProMoST 4.139
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.935
Rodwell 3.783
Grimsley 3.643
Solomon 3.961
Lehninger 3.923
Nozaki 4.088
DTASelect 4.368
Thurlkill 3.795
EMBOSS 3.935
Sillero 4.088
Patrickios 0.871
IPC_peptide 3.961
IPC2_peptide 4.062
IPC2.peptide.svr19 3.973
Protein with the highest isoelectric point:
>tr|A0A6B0U7J8|A0A6B0U7J8_9RHOB Cell division protein ZapE OS=Oceanomicrobium pacificus OX=2692916 GN=zapE PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 10.31 QPSNLVRR12 pKa = 11.84 KK13 pKa = 8.96 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.62 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATKK25 pKa = 10.45 NGRR28 pKa = 11.84 KK29 pKa = 8.78 ILNRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 KK40 pKa = 9.25 KK41 pKa = 10.65 LSAA44 pKa = 3.91
Molecular weight: 5.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3093
0
3093
998086
36
10282
322.7
34.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.91 ± 0.065
0.864 ± 0.017
6.628 ± 0.053
5.613 ± 0.043
3.715 ± 0.033
8.963 ± 0.052
1.941 ± 0.027
5.002 ± 0.038
2.768 ± 0.04
10.205 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.643 ± 0.03
2.426 ± 0.032
5.287 ± 0.042
2.952 ± 0.021
7.014 ± 0.061
4.975 ± 0.04
5.333 ± 0.057
7.225 ± 0.039
1.371 ± 0.022
2.163 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here