Bacteroides phage B40-8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5TRA2|B5TRA2_9CAUD Putative essential recombination function protein OS=Bacteroides phage B40-8 OX=99179 GN=B40-8018 PE=4 SV=1
MM1 pKa = 7.32QIKK4 pKa = 10.05KK5 pKa = 10.54DD6 pKa = 3.96RR7 pKa = 11.84IYY9 pKa = 11.21KK10 pKa = 10.53LLVQACKK17 pKa = 10.82NEE19 pKa = 4.8GISFSYY25 pKa = 10.33EE26 pKa = 3.87KK27 pKa = 10.91LVLSLNKK34 pKa = 10.63YY35 pKa = 9.31IDD37 pKa = 3.73EE38 pKa = 4.77DD39 pKa = 4.06EE40 pKa = 4.3EE41 pKa = 5.5DD42 pKa = 3.9SVFGCKK48 pKa = 9.63ISDD51 pKa = 3.28IDD53 pKa = 3.63ISIAKK58 pKa = 9.1HH59 pKa = 5.46ISVDD63 pKa = 3.03ICGTLALSNVICQLTCIGAGDD84 pKa = 4.33CPNCGGLLRR93 pKa = 11.84LIEE96 pKa = 4.6SYY98 pKa = 11.1PKK100 pKa = 10.33FSKK103 pKa = 10.48QYY105 pKa = 10.14CDD107 pKa = 4.02RR108 pKa = 11.84DD109 pKa = 3.91CEE111 pKa = 4.15PDD113 pKa = 3.43RR114 pKa = 11.84EE115 pKa = 4.2EE116 pKa = 4.24EE117 pKa = 4.13NVYY120 pKa = 10.87EE121 pKa = 4.98CLTCGKK127 pKa = 10.03EE128 pKa = 4.16VILL131 pKa = 5.11

Molecular weight: 14.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5TR96|B5TR96_9CAUD Hypothetical phage protein OS=Bacteroides phage B40-8 OX=99179 GN=B40-8012 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 10.48AKK4 pKa = 8.83ITAIVAFSFLFLLIVCLIRR23 pKa = 11.84YY24 pKa = 6.61NAKK27 pKa = 10.01LRR29 pKa = 11.84EE30 pKa = 4.12EE31 pKa = 4.0NGILNRR37 pKa = 11.84NVSVLTTQNVAYY49 pKa = 8.57RR50 pKa = 11.84TEE52 pKa = 4.2SGKK55 pKa = 10.73SAMKK59 pKa = 9.94TDD61 pKa = 3.83EE62 pKa = 4.75LNLTLRR68 pKa = 11.84QYY70 pKa = 11.61RR71 pKa = 11.84NTLQGKK77 pKa = 9.94DD78 pKa = 3.47STIKK82 pKa = 10.47DD83 pKa = 3.83LKK85 pKa = 11.01QSIKK89 pKa = 10.34DD90 pKa = 3.91LKK92 pKa = 10.71SHH94 pKa = 6.4TSIQTSTEE102 pKa = 4.3SVFSGSLRR110 pKa = 11.84DD111 pKa = 3.76SIIIRR116 pKa = 11.84DD117 pKa = 3.85SLVIDD122 pKa = 3.31TMKK125 pKa = 10.58CVNIRR130 pKa = 11.84SKK132 pKa = 10.38WLDD135 pKa = 3.25LSGCIDD141 pKa = 3.77SNGTFAGTTVTRR153 pKa = 11.84DD154 pKa = 3.02SLEE157 pKa = 3.76ILNIEE162 pKa = 3.82HH163 pKa = 7.56RR164 pKa = 11.84KK165 pKa = 8.82RR166 pKa = 11.84FLWFRR171 pKa = 11.84LKK173 pKa = 10.21KK174 pKa = 9.19VKK176 pKa = 10.22YY177 pKa = 9.62RR178 pKa = 11.84EE179 pKa = 4.21FIVTSKK185 pKa = 11.05NPYY188 pKa = 8.85TEE190 pKa = 3.55ITGFNVTTIIKK201 pKa = 10.16

Molecular weight: 22.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

46

0

46

12462

81

1897

270.9

30.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.074 ± 0.514

1.316 ± 0.191

5.898 ± 0.24

7.792 ± 0.429

4.478 ± 0.269

6.171 ± 0.468

1.388 ± 0.152

6.885 ± 0.231

8.402 ± 0.468

7.8 ± 0.265

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.279 ± 0.175

5.778 ± 0.234

2.921 ± 0.258

3.788 ± 0.29

4.614 ± 0.286

6.444 ± 0.244

6.115 ± 0.395

6.219 ± 0.289

0.955 ± 0.099

4.686 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski