Gordonia phage GordDuk1
Average proteome isoelectric point is 5.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0E3T6W6|A0A0E3T6W6_9CAUD Uncharacterized protein OS=Gordonia phage GordDuk1 OX=1622191 GN=GordDuk1_81 PE=4 SV=1
MM1 pKa = 8.3 AEE3 pKa = 4.05 MYY5 pKa = 8.2 EE6 pKa = 4.28 PPGSRR11 pKa = 11.84 EE12 pKa = 4.03 LMDD15 pKa = 4.16 EE16 pKa = 4.74 LSLAEE21 pKa = 4.39 QNPIAQGDD29 pKa = 3.97 GWSLLGPFAADD40 pKa = 3.31 GTYY43 pKa = 10.78 LPDD46 pKa = 4.21 LEE48 pKa = 6.21 AGVRR52 pKa = 11.84 YY53 pKa = 10.13 DD54 pKa = 3.38 SASNTVIIPP63 pKa = 3.71
Molecular weight: 6.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.859
IPC2_protein 3.668
IPC_protein 3.516
Toseland 3.35
ProMoST 3.719
Dawson 3.516
Bjellqvist 3.694
Wikipedia 3.478
Rodwell 3.376
Grimsley 3.274
Solomon 3.452
Lehninger 3.414
Nozaki 3.681
DTASelect 3.795
Thurlkill 3.439
EMBOSS 3.49
Sillero 3.643
Patrickios 1.799
IPC_peptide 3.452
IPC2_peptide 3.605
IPC2.peptide.svr19 3.665
Protein with the highest isoelectric point:
>tr|A0A0E3T645|A0A0E3T645_9CAUD DNA binding domain protein OS=Gordonia phage GordDuk1 OX=1622191 GN=GordDuk1_58 PE=4 SV=1
MM1 pKa = 7.2 QNQDD5 pKa = 3.27 MRR7 pKa = 11.84 HH8 pKa = 5.16 SSEE11 pKa = 3.84 MAKK14 pKa = 10.5 YY15 pKa = 9.78 LAIASGLTGKK25 pKa = 10.09 RR26 pKa = 11.84 QEE28 pKa = 3.91 LQAAKK33 pKa = 10.09 KK34 pKa = 10.52 AKK36 pKa = 10.4 DD37 pKa = 3.22 KK38 pKa = 11.1 AKK40 pKa = 10.43 RR41 pKa = 11.84 RR42 pKa = 11.84 AKK44 pKa = 10.25 RR45 pKa = 11.84 KK46 pKa = 7.73 RR47 pKa = 11.84 TGRR50 pKa = 3.49
Molecular weight: 5.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.208
IPC2_protein 10.116
IPC_protein 11.228
Toseland 11.52
ProMoST 11.886
Dawson 11.535
Bjellqvist 11.389
Wikipedia 11.886
Rodwell 11.725
Grimsley 11.564
Solomon 11.886
Lehninger 11.813
Nozaki 11.491
DTASelect 11.389
Thurlkill 11.506
EMBOSS 11.974
Sillero 11.506
Patrickios 11.462
IPC_peptide 11.901
IPC2_peptide 10.745
IPC2.peptide.svr19 8.95
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
97
0
97
23139
40
3272
238.5
26.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.058 ± 0.799
0.985 ± 0.162
6.513 ± 0.459
6.422 ± 0.335
3.95 ± 0.13
7.662 ± 0.436
1.673 ± 0.173
5.804 ± 0.187
5.683 ± 0.253
7.36 ± 0.235
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.818 ± 0.134
5.022 ± 0.18
4.819 ± 0.332
3.799 ± 0.216
5.087 ± 0.23
6.673 ± 0.276
5.744 ± 0.228
6.5 ± 0.222
1.5 ± 0.115
2.93 ± 0.183
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here