Gordonia phage GordDuk1 
Average proteome isoelectric point is 5.99 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A0E3T6W6|A0A0E3T6W6_9CAUD Uncharacterized protein OS=Gordonia phage GordDuk1 OX=1622191 GN=GordDuk1_81 PE=4 SV=1MM1 pKa = 8.3  AEE3 pKa = 4.05  MYY5 pKa = 8.2  EE6 pKa = 4.28  PPGSRR11 pKa = 11.84  EE12 pKa = 4.03  LMDD15 pKa = 4.16  EE16 pKa = 4.74  LSLAEE21 pKa = 4.39  QNPIAQGDD29 pKa = 3.97  GWSLLGPFAADD40 pKa = 3.31  GTYY43 pKa = 10.78  LPDD46 pKa = 4.21  LEE48 pKa = 6.21  AGVRR52 pKa = 11.84  YY53 pKa = 10.13  DD54 pKa = 3.38  SASNTVIIPP63 pKa = 3.71  
 6.77 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.859 
IPC2_protein 3.668 
IPC_protein 3.516 
Toseland    3.35 
ProMoST     3.719 
Dawson      3.516 
Bjellqvist  3.694 
Wikipedia   3.478 
Rodwell     3.376 
Grimsley    3.274 
Solomon     3.452 
Lehninger   3.414 
Nozaki      3.681 
DTASelect   3.795 
Thurlkill   3.439 
EMBOSS      3.49 
Sillero     3.643 
Patrickios  1.799 
IPC_peptide 3.452 
IPC2_peptide  3.605 
IPC2.peptide.svr19  3.665 
 Protein with the highest isoelectric point: 
>tr|A0A0E3T645|A0A0E3T645_9CAUD DNA binding domain protein OS=Gordonia phage GordDuk1 OX=1622191 GN=GordDuk1_58 PE=4 SV=1MM1 pKa = 7.2  QNQDD5 pKa = 3.27  MRR7 pKa = 11.84  HH8 pKa = 5.16  SSEE11 pKa = 3.84  MAKK14 pKa = 10.5  YY15 pKa = 9.78  LAIASGLTGKK25 pKa = 10.09  RR26 pKa = 11.84  QEE28 pKa = 3.91  LQAAKK33 pKa = 10.09  KK34 pKa = 10.52  AKK36 pKa = 10.4  DD37 pKa = 3.22  KK38 pKa = 11.1  AKK40 pKa = 10.43  RR41 pKa = 11.84  RR42 pKa = 11.84  AKK44 pKa = 10.25  RR45 pKa = 11.84  KK46 pKa = 7.73  RR47 pKa = 11.84  TGRR50 pKa = 3.49  
 5.73 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.208 
IPC2_protein 10.116 
IPC_protein 11.228 
Toseland    11.52 
ProMoST     11.886 
Dawson      11.535 
Bjellqvist  11.389 
Wikipedia   11.886 
Rodwell     11.725 
Grimsley    11.564 
Solomon     11.886 
Lehninger   11.813 
Nozaki      11.491 
DTASelect   11.389 
Thurlkill   11.506 
EMBOSS      11.974 
Sillero     11.506 
Patrickios  11.462 
IPC_peptide 11.901 
IPC2_peptide  10.745 
IPC2.peptide.svr19  8.95 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        97 
0
97 
23139
40
3272
238.5
26.37
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        9.058 ± 0.799
0.985 ± 0.162
6.513 ± 0.459
6.422 ± 0.335
3.95 ± 0.13
7.662 ± 0.436
1.673 ± 0.173
5.804 ± 0.187
5.683 ± 0.253
7.36 ± 0.235
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.818 ± 0.134
5.022 ± 0.18
4.819 ± 0.332
3.799 ± 0.216
5.087 ± 0.23
6.673 ± 0.276
5.744 ± 0.228
6.5 ± 0.222
1.5 ± 0.115
2.93 ± 0.183
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here