Paracoccus contaminans
Average proteome isoelectric point is 6.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2745 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W6CX93|A0A1W6CX93_9RHOB MBL fold hydrolase OS=Paracoccus contaminans OX=1945662 GN=B0A89_07380 PE=4 SV=1
MM1 pKa = 7.87 DD2 pKa = 3.63 QPQTQSSAPNQGEE15 pKa = 4.1 PVIAPSSTEE24 pKa = 3.7 HH25 pKa = 6.79 PLYY28 pKa = 10.85 DD29 pKa = 3.84 AVVEE33 pKa = 4.4 ACRR36 pKa = 11.84 TVYY39 pKa = 10.74 DD40 pKa = 3.74 PEE42 pKa = 5.01 IPVNIFDD49 pKa = 4.84 LGLVYY54 pKa = 10.04 TIAIDD59 pKa = 3.45 DD60 pKa = 3.83 TDD62 pKa = 3.39 AVRR65 pKa = 11.84 IMMTLTAPGCPVAGEE80 pKa = 4.15 MPGWVADD87 pKa = 4.24 AVNALPGVRR96 pKa = 11.84 QVDD99 pKa = 3.57 VEE101 pKa = 4.52 MTFQPPWGMEE111 pKa = 3.86 MMSDD115 pKa = 3.74 EE116 pKa = 4.65 ARR118 pKa = 11.84 LEE120 pKa = 4.14 LGFMM124 pKa = 4.54
Molecular weight: 13.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.769
IPC2_protein 3.719
IPC_protein 3.656
Toseland 3.452
ProMoST 3.834
Dawson 3.656
Bjellqvist 3.808
Wikipedia 3.592
Rodwell 3.49
Grimsley 3.376
Solomon 3.63
Lehninger 3.579
Nozaki 3.783
DTASelect 3.961
Thurlkill 3.528
EMBOSS 3.605
Sillero 3.783
Patrickios 1.837
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.733
Protein with the highest isoelectric point:
>tr|A0A1W6CVM6|A0A1W6CVM6_9RHOB Uncharacterized protein OS=Paracoccus contaminans OX=1945662 GN=B0A89_04025 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.37 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.36 GGRR28 pKa = 11.84 KK29 pKa = 8.97 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.97 VLSAA44 pKa = 4.11
Molecular weight: 5.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2745
0
2745
868674
33
2843
316.5
33.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.841 ± 0.087
0.801 ± 0.016
5.95 ± 0.041
5.128 ± 0.044
3.176 ± 0.027
9.469 ± 0.049
1.978 ± 0.022
4.676 ± 0.034
2.241 ± 0.035
10.047 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.663 ± 0.027
2.12 ± 0.025
5.851 ± 0.042
3.166 ± 0.023
8.055 ± 0.055
4.551 ± 0.034
4.947 ± 0.035
7.002 ± 0.044
1.466 ± 0.021
1.872 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here