BeAn 58058 virus
Average proteome isoelectric point is 7.04
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 209 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3IZQ8|A0A1L3IZQ8_9POXV DNA ligase OS=BeAn 58058 virus OX=67082 GN=BAV00177 PE=4 SV=1
MM1 pKa = 8.13 DD2 pKa = 4.94 SGSIFVTIVTIIIAIILFGYY22 pKa = 9.51 GVSICLCYY30 pKa = 10.55 SIPLLYY36 pKa = 10.5 KK37 pKa = 10.35 KK38 pKa = 10.07 EE39 pKa = 4.57 SKK41 pKa = 11.02 DD42 pKa = 4.15 KK43 pKa = 10.38 MIKK46 pKa = 9.62 LNQMYY51 pKa = 10.29 FGTLDD56 pKa = 3.4 NKK58 pKa = 10.74 KK59 pKa = 9.92 SIQYY63 pKa = 6.18 VTSYY67 pKa = 11.44 EE68 pKa = 3.95 EE69 pKa = 3.87 TDD71 pKa = 4.92 DD72 pKa = 3.87 YY73 pKa = 11.81 NQDD76 pKa = 3.08 EE77 pKa = 4.96 FIEE80 pKa = 4.67 DD81 pKa = 3.54 NEE83 pKa = 4.18
Molecular weight: 9.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.033
IPC2_protein 4.329
IPC_protein 4.202
Toseland 4.012
ProMoST 4.266
Dawson 4.164
Bjellqvist 4.406
Wikipedia 4.088
Rodwell 4.037
Grimsley 3.923
Solomon 4.151
Lehninger 4.113
Nozaki 4.291
DTASelect 4.469
Thurlkill 4.05
EMBOSS 4.088
Sillero 4.317
Patrickios 0.896
IPC_peptide 4.164
IPC2_peptide 4.291
IPC2.peptide.svr19 4.279
Protein with the highest isoelectric point:
>tr|A0A1L3IZU1|A0A1L3IZU1_9POXV Ig-like domain-containing protein OS=BeAn 58058 virus OX=67082 GN=BAV00214 PE=4 SV=1
MM1 pKa = 7.36 YY2 pKa = 10.28 SIEE5 pKa = 4.3 NFKK8 pKa = 9.09 MWYY11 pKa = 10.11 SILCVKK17 pKa = 10.55 RR18 pKa = 11.84 IDD20 pKa = 4.31 LYY22 pKa = 10.79 IPKK25 pKa = 10.06 FKK27 pKa = 10.07 IKK29 pKa = 10.2 KK30 pKa = 9.02 YY31 pKa = 9.3 RR32 pKa = 11.84 TII34 pKa = 3.62
Molecular weight: 4.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.133
IPC2_protein 9.458
IPC_protein 9.414
Toseland 9.911
ProMoST 9.648
Dawson 10.16
Bjellqvist 9.838
Wikipedia 10.335
Rodwell 10.613
Grimsley 10.233
Solomon 10.189
Lehninger 10.16
Nozaki 9.926
DTASelect 9.838
Thurlkill 9.984
EMBOSS 10.321
Sillero 10.072
Patrickios 10.292
IPC_peptide 10.189
IPC2_peptide 8.551
IPC2.peptide.svr19 8.321
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
209
0
209
47411
30
1315
226.8
26.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.369 ± 0.096
2.035 ± 0.099
6.385 ± 0.134
5.033 ± 0.104
5.174 ± 0.136
3.398 ± 0.113
1.626 ± 0.071
11.78 ± 0.207
9.076 ± 0.151
8.705 ± 0.161
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.345 ± 0.081
9.209 ± 0.205
2.776 ± 0.118
1.801 ± 0.08
3.082 ± 0.104
7.477 ± 0.175
5.716 ± 0.151
5.406 ± 0.125
0.607 ± 0.041
6.001 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here