Flavobacteriaceae bacterium F202Z8

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; unclassified Flavobacteriaceae

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3847 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B7SYN8|A0A5B7SYN8_9FLAO Response regulator transcription factor OS=Flavobacteriaceae bacterium F202Z8 OX=2583587 GN=FGM00_08155 PE=4 SV=1
MM1 pKa = 7.7LSGALRR7 pKa = 11.84KK8 pKa = 10.06YY9 pKa = 10.62GLSFLLGACLVFMLLLASSCDD30 pKa = 3.7NEE32 pKa = 5.08DD33 pKa = 4.08VLDD36 pKa = 4.47LDD38 pKa = 4.19EE39 pKa = 4.5TAMTNDD45 pKa = 5.4DD46 pKa = 4.66IVTCTDD52 pKa = 3.75GIMNGDD58 pKa = 3.68EE59 pKa = 4.08TGIDD63 pKa = 3.54CGGACTPCEE72 pKa = 4.0AGEE75 pKa = 4.06EE76 pKa = 4.04LGRR79 pKa = 11.84RR80 pKa = 11.84AEE82 pKa = 4.69LYY84 pKa = 8.43VTNNANGDD92 pKa = 3.15ISRR95 pKa = 11.84YY96 pKa = 9.82SVTGDD101 pKa = 3.04SLTTFTTASTAAEE114 pKa = 4.59GIYY117 pKa = 10.34YY118 pKa = 10.2SSKK121 pKa = 11.17DD122 pKa = 3.46GVLVQASRR130 pKa = 11.84SGLQLDD136 pKa = 4.77AYY138 pKa = 10.67SGIAGIMEE146 pKa = 5.06DD147 pKa = 3.74SALDD151 pKa = 3.52ASFSSTTDD159 pKa = 3.18LEE161 pKa = 4.65SPRR164 pKa = 11.84EE165 pKa = 3.88LAVNGDD171 pKa = 3.99MYY173 pKa = 11.42VVSDD177 pKa = 3.4NGSNKK182 pKa = 9.43FFVYY186 pKa = 9.63TKK188 pKa = 10.63NGDD191 pKa = 3.99SFSLTSTVEE200 pKa = 3.5IPFPVWGITFRR211 pKa = 11.84GSDD214 pKa = 3.84LYY216 pKa = 11.45AVVDD220 pKa = 3.94NSSDD224 pKa = 3.39LAVFNNFQASISAPVCALNPSKK246 pKa = 10.57RR247 pKa = 11.84VVIEE251 pKa = 4.47GIVRR255 pKa = 11.84THH257 pKa = 5.78GLVYY261 pKa = 10.66NAADD265 pKa = 5.25DD266 pKa = 3.85IMIMTDD272 pKa = 3.02IGDD275 pKa = 4.14AANTTDD281 pKa = 4.76DD282 pKa = 3.99GAFHH286 pKa = 6.81VISEE290 pKa = 4.51FAATFDD296 pKa = 4.19LLSDD300 pKa = 3.91GEE302 pKa = 4.62VYY304 pKa = 10.39PLSRR308 pKa = 11.84QVRR311 pKa = 11.84VAGPSTMMGNPIDD324 pKa = 3.64VAYY327 pKa = 10.39DD328 pKa = 3.47SEE330 pKa = 4.45EE331 pKa = 3.84NAVYY335 pKa = 10.19ISEE338 pKa = 4.2VGNGKK343 pKa = 9.25VLGFTAQGGGDD354 pKa = 3.43RR355 pKa = 11.84APWFVKK361 pKa = 10.28DD362 pKa = 4.32LPAASSIYY370 pKa = 10.13FSSDD374 pKa = 2.82EE375 pKa = 4.15TDD377 pKa = 3.54DD378 pKa = 4.77DD379 pKa = 4.53LGEE382 pKa = 3.85EE383 pKa = 4.06AMAGNTVLYY392 pKa = 8.44ATSTANGNIYY402 pKa = 10.65VYY404 pKa = 10.98DD405 pKa = 3.73AMGNALKK412 pKa = 9.61TVTSQSEE419 pKa = 4.26SSEE422 pKa = 4.1GIYY425 pKa = 8.85YY426 pKa = 8.18TALDD430 pKa = 5.29DD431 pKa = 4.69ILVQASRR438 pKa = 11.84SALQLEE444 pKa = 5.62GYY446 pKa = 10.7AEE448 pKa = 4.84FSTVPDD454 pKa = 3.97GAIGAASFTSSKK466 pKa = 11.29DD467 pKa = 3.23LMSPRR472 pKa = 11.84EE473 pKa = 3.64IAVYY477 pKa = 10.2GNKK480 pKa = 9.53IVVADD485 pKa = 3.52NAEE488 pKa = 4.23NIFYY492 pKa = 10.59VYY494 pKa = 10.37SHH496 pKa = 7.21DD497 pKa = 3.67MFSFSLINTFRR508 pKa = 11.84FEE510 pKa = 4.58CRR512 pKa = 11.84GIWGITFKK520 pKa = 11.18GDD522 pKa = 3.37DD523 pKa = 3.96LLAVVDD529 pKa = 4.03NTGDD533 pKa = 3.55LAIFSDD539 pKa = 4.63FFTNYY544 pKa = 9.08TVDD547 pKa = 3.81GNITPEE553 pKa = 4.49KK554 pKa = 9.97IITIQGIVRR563 pKa = 11.84THH565 pKa = 6.85GIDD568 pKa = 3.34YY569 pKa = 10.78SAADD573 pKa = 4.69DD574 pKa = 4.54LLVMTDD580 pKa = 3.89IGDD583 pKa = 4.06AANGTDD589 pKa = 4.38DD590 pKa = 5.69GGFQLIQEE598 pKa = 4.74FSAKK602 pKa = 10.05LDD604 pKa = 4.57AISNGGTLVLSDD616 pKa = 3.53QTRR619 pKa = 11.84VSGPGTMMGNPIDD632 pKa = 3.51IAYY635 pKa = 10.42DD636 pKa = 3.59NDD638 pKa = 3.75SKK640 pKa = 10.11TVYY643 pKa = 9.82IAEE646 pKa = 4.33IGNGKK651 pKa = 9.05ILGFSDD657 pKa = 3.52VLNASGDD664 pKa = 3.71VAPTFNWDD672 pKa = 3.21LNQASSIFLYY682 pKa = 11.12NNN684 pKa = 3.13

Molecular weight: 72.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B7SPQ6|A0A5B7SPQ6_9FLAO Acyl-CoA carboxylase subunit beta OS=Flavobacteriaceae bacterium F202Z8 OX=2583587 GN=FGM00_08650 PE=4 SV=1
MM1 pKa = 7.67RR2 pKa = 11.84KK3 pKa = 9.86AEE5 pKa = 3.98NRR7 pKa = 11.84EE8 pKa = 3.58QRR10 pKa = 11.84MEE12 pKa = 4.01NGEE15 pKa = 3.83KK16 pKa = 10.14RR17 pKa = 11.84KK18 pKa = 9.81EE19 pKa = 3.81KK20 pKa = 10.74RR21 pKa = 11.84EE22 pKa = 3.6WRR24 pKa = 11.84MEE26 pKa = 3.76NGEE29 pKa = 3.87WRR31 pKa = 11.84KK32 pKa = 9.42FRR34 pKa = 11.84VKK36 pKa = 10.54NSNPNRR42 pKa = 11.84YY43 pKa = 6.38TLRR46 pKa = 11.84LFLVSCWRR54 pKa = 11.84MVCSRR59 pKa = 11.84IKK61 pKa = 10.6FKK63 pKa = 10.99LLSKK67 pKa = 10.91GG68 pKa = 3.37

Molecular weight: 8.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3847

0

3847

1374296

38

4852

357.2

40.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.062 ± 0.035

0.745 ± 0.014

5.978 ± 0.053

6.692 ± 0.037

5.043 ± 0.032

6.99 ± 0.048

1.781 ± 0.021

7.124 ± 0.032

6.858 ± 0.065

9.306 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.285 ± 0.023

5.488 ± 0.04

3.647 ± 0.026

3.38 ± 0.022

3.919 ± 0.03

6.335 ± 0.032

5.953 ± 0.059

6.418 ± 0.038

1.14 ± 0.014

3.855 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski