Aureliella helgolandensis

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Aureliella

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6357 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A518G0C3|A0A518G0C3_9BACT Uncharacterized protein OS=Aureliella helgolandensis OX=2527968 GN=Q31a_03310 PE=4 SV=1
MM1 pKa = 8.25ILMRR5 pKa = 11.84YY6 pKa = 8.98LMCTLLIGSSLQTAMADD23 pKa = 3.32EE24 pKa = 4.81LVFTLDD30 pKa = 3.37AARR33 pKa = 11.84SSLTLAGSTTVGDD46 pKa = 3.77PVNNTFAYY54 pKa = 7.33TAQGANSLITSYY66 pKa = 11.19GGAITVDD73 pKa = 3.71VSDD76 pKa = 4.9LLNPLSITFLSSNVDD91 pKa = 3.33ANVNGNWLPSDD102 pKa = 5.15DD103 pKa = 4.28IVPTEE108 pKa = 3.86PASAGDD114 pKa = 3.34YY115 pKa = 10.51GIRR118 pKa = 11.84IDD120 pKa = 5.93AILTNGKK127 pKa = 8.09MRR129 pKa = 11.84NVDD132 pKa = 4.03FNIAAASTSVTGGSFAVSGQTFGFNSGIIDD162 pKa = 3.84VYY164 pKa = 10.65STALTAGNSIDD175 pKa = 3.68FTNAGTLAGTGLNVAAGTGTYY196 pKa = 9.88SVSGGIATLTIPVAVTLPYY215 pKa = 10.43TFGPTSEE222 pKa = 4.21PTVEE226 pKa = 4.47GSQTYY231 pKa = 8.97TGTLVGVAAVPEE243 pKa = 4.33PSSLALLSLTAGCIGFVRR261 pKa = 11.84RR262 pKa = 11.84RR263 pKa = 11.84SPAA266 pKa = 2.97

Molecular weight: 26.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A518G8B4|A0A518G8B4_9BACT Type II secretion system protein G OS=Aureliella helgolandensis OX=2527968 GN=pulG_2 PE=4 SV=1
MM1 pKa = 7.56GAGNTSKK8 pKa = 10.72LRR10 pKa = 11.84WFLSIHH16 pKa = 6.73AIVVAPKK23 pKa = 10.37INLQTTWLKK32 pKa = 9.98IHH34 pKa = 6.36SLSGLLFRR42 pKa = 11.84QPVTRR47 pKa = 11.84RR48 pKa = 11.84GTIGTVPGSMAPRR61 pKa = 11.84AVRR64 pKa = 11.84ITQPVRR70 pKa = 11.84RR71 pKa = 11.84PNRR74 pKa = 3.4

Molecular weight: 8.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6357

0

6357

2352101

30

8810

370.0

40.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.918 ± 0.031

1.209 ± 0.014

5.553 ± 0.035

6.146 ± 0.03

3.623 ± 0.016

7.585 ± 0.04

2.236 ± 0.018

4.852 ± 0.022

3.334 ± 0.023

10.338 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.125 ± 0.017

3.236 ± 0.024

5.291 ± 0.024

4.605 ± 0.026

6.415 ± 0.031

7.021 ± 0.029

5.568 ± 0.034

6.937 ± 0.023

1.581 ± 0.015

2.427 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski