Streptococcus phage CHPC873
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3G8F772|A0A3G8F772_9CAUD Uncharacterized protein OS=Streptococcus phage CHPC873 OX=2365042 GN=CHPC873_0044 PE=4 SV=1
MM1 pKa = 7.3 SVSKK5 pKa = 9.08 EE6 pKa = 4.26 TIMQTLNLDD15 pKa = 3.8 EE16 pKa = 4.97 TDD18 pKa = 3.78 DD19 pKa = 3.8 TALIPAYY26 pKa = 9.65 IEE28 pKa = 3.9 SARR31 pKa = 11.84 QYY33 pKa = 10.3 VVNSVGDD40 pKa = 3.54 DD41 pKa = 3.37 PKK43 pKa = 10.95 FYY45 pKa = 11.07 NLDD48 pKa = 3.41 SVRR51 pKa = 11.84 ALFDD55 pKa = 3.34 TAVIALTSSYY65 pKa = 8.66 FTYY68 pKa = 10.07 RR69 pKa = 11.84 VALTDD74 pKa = 3.37 TATYY78 pKa = 9.41 PVNLTLNSIIGQLRR92 pKa = 11.84 GLYY95 pKa = 8.66 ATYY98 pKa = 10.56 SEE100 pKa = 4.46 EE101 pKa = 5.06 RR102 pKa = 11.84 GDD104 pKa = 3.53
Molecular weight: 11.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.863
IPC2_protein 4.151
IPC_protein 4.062
Toseland 3.859
ProMoST 4.139
Dawson 4.062
Bjellqvist 4.279
Wikipedia 3.999
Rodwell 3.897
Grimsley 3.77
Solomon 4.05
Lehninger 3.999
Nozaki 4.177
DTASelect 4.406
Thurlkill 3.923
EMBOSS 4.012
Sillero 4.19
Patrickios 1.964
IPC_peptide 4.037
IPC2_peptide 4.164
IPC2.peptide.svr19 4.1
Protein with the highest isoelectric point:
>tr|A0A3G8F9X9|A0A3G8F9X9_9CAUD Single-stranded DNA-binding protein OS=Streptococcus phage CHPC873 OX=2365042 GN=CHPC873_0037 PE=4 SV=1
MM1 pKa = 7.79 KK2 pKa = 10.47 NRR4 pKa = 11.84 LLGSRR9 pKa = 11.84 YY10 pKa = 8.35 TDD12 pKa = 4.05 AIKK15 pKa = 10.95 NNCGTANKK23 pKa = 9.21 MSNIYY28 pKa = 10.64 NKK30 pKa = 10.32 LNKK33 pKa = 10.0 DD34 pKa = 3.58 SLRR37 pKa = 11.84 EE38 pKa = 3.88 IHH40 pKa = 6.32 SALYY44 pKa = 10.59 GLLTAGYY51 pKa = 9.61 DD52 pKa = 3.25 ISNMRR57 pKa = 11.84 NIEE60 pKa = 4.04 EE61 pKa = 4.16 LEE63 pKa = 4.13 KK64 pKa = 11.06 YY65 pKa = 10.94 VNLKK69 pKa = 10.1 KK70 pKa = 10.82 SRR72 pKa = 11.84 GQLLNVSSNDD82 pKa = 2.51 IKK84 pKa = 10.9 LYY86 pKa = 10.63 HH87 pKa = 6.74 KK88 pKa = 10.64 LFVIRR93 pKa = 11.84 FGKK96 pKa = 10.2
Molecular weight: 11.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.117
IPC2_protein 9.37
IPC_protein 9.326
Toseland 9.984
ProMoST 9.736
Dawson 10.189
Bjellqvist 9.853
Wikipedia 10.35
Rodwell 10.672
Grimsley 10.262
Solomon 10.218
Lehninger 10.189
Nozaki 9.97
DTASelect 9.853
Thurlkill 10.028
EMBOSS 10.379
Sillero 10.101
Patrickios 10.292
IPC_peptide 10.218
IPC2_peptide 8.448
IPC2.peptide.svr19 8.311
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
51
0
51
11775
38
1600
230.9
26.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.565 ± 0.532
0.679 ± 0.204
6.454 ± 0.244
6.59 ± 0.466
4.28 ± 0.215
6.616 ± 0.407
1.58 ± 0.121
6.879 ± 0.262
8.569 ± 0.546
7.856 ± 0.348
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.403 ± 0.197
6.675 ± 0.266
2.896 ± 0.165
3.958 ± 0.188
4.093 ± 0.255
6.268 ± 0.254
6.089 ± 0.319
6.038 ± 0.294
1.486 ± 0.136
4.025 ± 0.272
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here