Amino acid dipepetide frequency for Streptococcus phage CHPC873

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.482AlaAla: 3.482 ± 1.062
0.34AlaCys: 0.34 ± 0.216
4.671AlaAsp: 4.671 ± 0.908
3.567AlaGlu: 3.567 ± 0.521
2.378AlaPhe: 2.378 ± 0.552
3.907AlaGly: 3.907 ± 0.636
1.104AlaHis: 1.104 ± 0.331
4.841AlaIle: 4.841 ± 0.672
6.795AlaLys: 6.795 ± 1.133
5.521AlaLeu: 5.521 ± 0.697
2.038AlaMet: 2.038 ± 0.463
4.332AlaAsn: 4.332 ± 0.681
1.529AlaPro: 1.529 ± 0.368
2.378AlaGln: 2.378 ± 0.485
2.803AlaArg: 2.803 ± 0.372
4.162AlaSer: 4.162 ± 0.629
4.162AlaThr: 4.162 ± 0.635
3.567AlaVal: 3.567 ± 0.607
1.274AlaTrp: 1.274 ± 0.492
2.803AlaTyr: 2.803 ± 0.497
0.0AlaXaa: 0.0 ± 0.0
Cys
0.17CysAla: 0.17 ± 0.108
0.0CysCys: 0.0 ± 0.0
0.595CysAsp: 0.595 ± 0.203
0.34CysGlu: 0.34 ± 0.166
0.34CysPhe: 0.34 ± 0.212
0.34CysGly: 0.34 ± 0.183
0.085CysHis: 0.085 ± 0.079
0.425CysIle: 0.425 ± 0.23
0.679CysLys: 0.679 ± 0.338
0.764CysLeu: 0.764 ± 0.281
0.17CysMet: 0.17 ± 0.122
0.51CysAsn: 0.51 ± 0.285
0.34CysPro: 0.34 ± 0.203
0.255CysGln: 0.255 ± 0.161
0.51CysArg: 0.51 ± 0.326
0.255CysSer: 0.255 ± 0.131
0.425CysThr: 0.425 ± 0.171
0.085CysVal: 0.085 ± 0.065
0.17CysTrp: 0.17 ± 0.134
0.34CysTyr: 0.34 ± 0.168
0.0CysXaa: 0.0 ± 0.0
Asp
3.058AspAla: 3.058 ± 0.476
0.679AspCys: 0.679 ± 0.266
3.822AspAsp: 3.822 ± 0.582
3.737AspGlu: 3.737 ± 0.606
4.247AspPhe: 4.247 ± 0.604
5.945AspGly: 5.945 ± 0.974
0.849AspHis: 0.849 ± 0.238
4.926AspIle: 4.926 ± 0.707
4.841AspLys: 4.841 ± 0.707
3.992AspLeu: 3.992 ± 0.689
1.784AspMet: 1.784 ± 0.479
5.096AspAsn: 5.096 ± 1.043
1.953AspPro: 1.953 ± 0.393
1.359AspGln: 1.359 ± 0.241
2.378AspArg: 2.378 ± 0.472
4.332AspSer: 4.332 ± 0.43
3.992AspThr: 3.992 ± 0.55
3.737AspVal: 3.737 ± 0.606
1.104AspTrp: 1.104 ± 0.28
2.718AspTyr: 2.718 ± 0.454
0.0AspXaa: 0.0 ± 0.0
Glu
4.077GluAla: 4.077 ± 0.528
0.34GluCys: 0.34 ± 0.142
3.397GluAsp: 3.397 ± 0.739
4.332GluGlu: 4.332 ± 0.85
2.463GluPhe: 2.463 ± 0.455
2.973GluGly: 2.973 ± 0.433
1.189GluHis: 1.189 ± 0.374
6.2GluIle: 6.2 ± 0.907
4.671GluLys: 4.671 ± 1.058
5.945GluLeu: 5.945 ± 0.726
1.953GluMet: 1.953 ± 0.376
4.756GluAsn: 4.756 ± 0.674
2.123GluPro: 2.123 ± 0.57
2.888GluGln: 2.888 ± 0.525
3.058GluArg: 3.058 ± 0.618
3.482GluSer: 3.482 ± 0.531
3.312GluThr: 3.312 ± 0.454
4.247GluVal: 4.247 ± 0.705
1.019GluTrp: 1.019 ± 0.251
3.482GluTyr: 3.482 ± 0.621
0.0GluXaa: 0.0 ± 0.0
Phe
2.803PheAla: 2.803 ± 0.471
0.085PheCys: 0.085 ± 0.087
3.482PheAsp: 3.482 ± 0.588
2.208PheGlu: 2.208 ± 0.545
1.614PhePhe: 1.614 ± 0.382
3.822PheGly: 3.822 ± 0.658
0.51PheHis: 0.51 ± 0.199
2.378PheIle: 2.378 ± 0.47
3.822PheLys: 3.822 ± 0.581
2.973PheLeu: 2.973 ± 0.457
0.679PheMet: 0.679 ± 0.272
4.332PheAsn: 4.332 ± 0.702
0.425PhePro: 0.425 ± 0.148
1.359PheGln: 1.359 ± 0.338
1.784PheArg: 1.784 ± 0.338
2.888PheSer: 2.888 ± 0.491
2.208PheThr: 2.208 ± 0.488
3.227PheVal: 3.227 ± 0.543
0.51PheTrp: 0.51 ± 0.167
1.699PheTyr: 1.699 ± 0.408
0.0PheXaa: 0.0 ± 0.0
Gly
3.567GlyAla: 3.567 ± 0.633
0.51GlyCys: 0.51 ± 0.234
4.162GlyAsp: 4.162 ± 0.599
4.077GlyGlu: 4.077 ± 0.624
3.567GlyPhe: 3.567 ± 0.587
4.501GlyGly: 4.501 ± 0.674
0.764GlyHis: 0.764 ± 0.238
4.841GlyIle: 4.841 ± 0.708
6.03GlyLys: 6.03 ± 0.762
5.606GlyLeu: 5.606 ± 0.87
1.274GlyMet: 1.274 ± 0.308
3.652GlyAsn: 3.652 ± 0.791
0.51GlyPro: 0.51 ± 0.215
2.548GlyGln: 2.548 ± 0.647
3.227GlyArg: 3.227 ± 0.528
4.671GlySer: 4.671 ± 0.703
4.332GlyThr: 4.332 ± 0.575
3.567GlyVal: 3.567 ± 0.53
1.699GlyTrp: 1.699 ± 0.509
3.058GlyTyr: 3.058 ± 0.505
0.0GlyXaa: 0.0 ± 0.0
His
0.679HisAla: 0.679 ± 0.295
0.0HisCys: 0.0 ± 0.0
1.189HisAsp: 1.189 ± 0.282
0.51HisGlu: 0.51 ± 0.199
0.849HisPhe: 0.849 ± 0.255
0.679HisGly: 0.679 ± 0.213
0.51HisHis: 0.51 ± 0.204
1.699HisIle: 1.699 ± 0.394
1.189HisLys: 1.189 ± 0.32
1.359HisLeu: 1.359 ± 0.254
0.255HisMet: 0.255 ± 0.176
0.679HisAsn: 0.679 ± 0.294
0.51HisPro: 0.51 ± 0.183
0.425HisGln: 0.425 ± 0.19
0.425HisArg: 0.425 ± 0.151
1.444HisSer: 1.444 ± 0.346
0.934HisThr: 0.934 ± 0.227
1.274HisVal: 1.274 ± 0.307
0.17HisTrp: 0.17 ± 0.123
1.019HisTyr: 1.019 ± 0.358
0.0HisXaa: 0.0 ± 0.0
Ile
5.181IleAla: 5.181 ± 0.845
0.425IleCys: 0.425 ± 0.184
5.521IleAsp: 5.521 ± 0.646
5.521IleGlu: 5.521 ± 0.879
1.614IlePhe: 1.614 ± 0.415
4.501IleGly: 4.501 ± 0.517
0.764IleHis: 0.764 ± 0.235
3.143IleIle: 3.143 ± 0.543
7.219IleLys: 7.219 ± 0.847
3.737IleLeu: 3.737 ± 0.691
1.784IleMet: 1.784 ± 0.388
4.586IleAsn: 4.586 ± 0.571
3.397IlePro: 3.397 ± 0.484
3.227IleGln: 3.227 ± 0.548
3.058IleArg: 3.058 ± 0.582
4.501IleSer: 4.501 ± 0.57
4.077IleThr: 4.077 ± 0.649
3.058IleVal: 3.058 ± 0.443
1.019IleTrp: 1.019 ± 0.255
2.463IleTyr: 2.463 ± 0.501
0.0IleXaa: 0.0 ± 0.0
Lys
6.71LysAla: 6.71 ± 0.798
0.34LysCys: 0.34 ± 0.255
4.671LysAsp: 4.671 ± 0.706
7.474LysGlu: 7.474 ± 0.885
3.227LysPhe: 3.227 ± 0.738
5.266LysGly: 5.266 ± 0.636
1.614LysHis: 1.614 ± 0.418
6.115LysIle: 6.115 ± 0.729
7.389LysLys: 7.389 ± 1.344
6.625LysLeu: 6.625 ± 0.936
2.718LysMet: 2.718 ± 0.631
5.945LysAsn: 5.945 ± 0.693
2.208LysPro: 2.208 ± 0.429
3.992LysGln: 3.992 ± 0.587
3.143LysArg: 3.143 ± 0.447
4.417LysSer: 4.417 ± 0.503
4.756LysThr: 4.756 ± 0.596
4.586LysVal: 4.586 ± 0.633
1.274LysTrp: 1.274 ± 0.271
3.227LysTyr: 3.227 ± 0.671
0.0LysXaa: 0.0 ± 0.0
Leu
6.285LeuAla: 6.285 ± 0.72
0.425LeuCys: 0.425 ± 0.206
5.351LeuAsp: 5.351 ± 0.66
6.455LeuGlu: 6.455 ± 1.098
3.312LeuPhe: 3.312 ± 0.451
5.351LeuGly: 5.351 ± 0.835
0.764LeuHis: 0.764 ± 0.294
4.332LeuIle: 4.332 ± 0.664
7.134LeuLys: 7.134 ± 0.755
5.181LeuLeu: 5.181 ± 0.81
2.378LeuMet: 2.378 ± 0.442
5.606LeuAsn: 5.606 ± 0.574
2.888LeuPro: 2.888 ± 0.44
2.888LeuGln: 2.888 ± 0.424
3.397LeuArg: 3.397 ± 0.628
4.926LeuSer: 4.926 ± 0.723
5.351LeuThr: 5.351 ± 0.829
3.652LeuVal: 3.652 ± 0.584
0.764LeuTrp: 0.764 ± 0.302
2.038LeuTyr: 2.038 ± 0.442
0.0LeuXaa: 0.0 ± 0.0
Met
2.378MetAla: 2.378 ± 0.406
0.0MetCys: 0.0 ± 0.0
0.934MetAsp: 0.934 ± 0.376
1.104MetGlu: 1.104 ± 0.339
1.019MetPhe: 1.019 ± 0.244
1.019MetGly: 1.019 ± 0.339
0.255MetHis: 0.255 ± 0.16
1.953MetIle: 1.953 ± 0.392
3.567MetLys: 3.567 ± 0.619
1.953MetLeu: 1.953 ± 0.315
0.255MetMet: 0.255 ± 0.158
0.679MetAsn: 0.679 ± 0.229
1.019MetPro: 1.019 ± 0.262
0.849MetGln: 0.849 ± 0.251
1.189MetArg: 1.189 ± 0.354
1.189MetSer: 1.189 ± 0.283
1.869MetThr: 1.869 ± 0.353
1.869MetVal: 1.869 ± 0.352
0.17MetTrp: 0.17 ± 0.097
0.764MetTyr: 0.764 ± 0.297
0.0MetXaa: 0.0 ± 0.0
Asn
5.351AsnAla: 5.351 ± 0.876
0.595AsnCys: 0.595 ± 0.258
4.247AsnAsp: 4.247 ± 0.474
4.077AsnGlu: 4.077 ± 0.759
2.633AsnPhe: 2.633 ± 0.477
6.285AsnGly: 6.285 ± 1.07
1.019AsnHis: 1.019 ± 0.25
3.737AsnIle: 3.737 ± 0.561
4.247AsnLys: 4.247 ± 0.624
5.775AsnLeu: 5.775 ± 0.525
1.614AsnMet: 1.614 ± 0.422
5.011AsnAsn: 5.011 ± 0.766
3.143AsnPro: 3.143 ± 0.609
2.463AsnGln: 2.463 ± 0.349
2.293AsnArg: 2.293 ± 0.529
4.417AsnSer: 4.417 ± 0.718
3.312AsnThr: 3.312 ± 0.512
3.907AsnVal: 3.907 ± 0.553
0.934AsnTrp: 0.934 ± 0.239
1.699AsnTyr: 1.699 ± 0.374
0.0AsnXaa: 0.0 ± 0.0
Pro
1.614ProAla: 1.614 ± 0.311
0.085ProCys: 0.085 ± 0.093
1.614ProAsp: 1.614 ± 0.382
2.123ProGlu: 2.123 ± 0.452
1.189ProPhe: 1.189 ± 0.259
1.529ProGly: 1.529 ± 0.337
0.425ProHis: 0.425 ± 0.169
1.444ProIle: 1.444 ± 0.336
3.227ProLys: 3.227 ± 0.502
2.378ProLeu: 2.378 ± 0.473
0.255ProMet: 0.255 ± 0.144
2.463ProAsn: 2.463 ± 0.428
0.679ProPro: 0.679 ± 0.3
1.444ProGln: 1.444 ± 0.256
0.595ProArg: 0.595 ± 0.242
2.123ProSer: 2.123 ± 0.332
2.718ProThr: 2.718 ± 0.419
1.614ProVal: 1.614 ± 0.454
0.34ProTrp: 0.34 ± 0.15
1.104ProTyr: 1.104 ± 0.362
0.0ProXaa: 0.0 ± 0.0
Gln
3.822GlnAla: 3.822 ± 0.484
0.34GlnCys: 0.34 ± 0.175
1.784GlnAsp: 1.784 ± 0.393
2.548GlnGlu: 2.548 ± 0.458
1.784GlnPhe: 1.784 ± 0.372
2.888GlnGly: 2.888 ± 0.569
0.595GlnHis: 0.595 ± 0.214
2.378GlnIle: 2.378 ± 0.533
3.227GlnLys: 3.227 ± 0.493
3.397GlnLeu: 3.397 ± 0.488
1.359GlnMet: 1.359 ± 0.334
2.378GlnAsn: 2.378 ± 0.36
0.425GlnPro: 0.425 ± 0.196
2.293GlnGln: 2.293 ± 0.44
1.104GlnArg: 1.104 ± 0.217
1.953GlnSer: 1.953 ± 0.304
3.058GlnThr: 3.058 ± 0.46
1.699GlnVal: 1.699 ± 0.306
0.595GlnTrp: 0.595 ± 0.163
1.953GlnTyr: 1.953 ± 0.411
0.0GlnXaa: 0.0 ± 0.0
Arg
1.699ArgAla: 1.699 ± 0.393
0.34ArgCys: 0.34 ± 0.273
2.208ArgAsp: 2.208 ± 0.497
2.378ArgGlu: 2.378 ± 0.4
2.208ArgPhe: 2.208 ± 0.484
2.463ArgGly: 2.463 ± 0.45
0.849ArgHis: 0.849 ± 0.232
3.227ArgIle: 3.227 ± 0.546
3.058ArgLys: 3.058 ± 0.517
3.397ArgLeu: 3.397 ± 0.569
1.019ArgMet: 1.019 ± 0.297
2.888ArgAsn: 2.888 ± 0.348
1.019ArgPro: 1.019 ± 0.241
1.699ArgGln: 1.699 ± 0.295
1.614ArgArg: 1.614 ± 0.443
1.869ArgSer: 1.869 ± 0.405
2.548ArgThr: 2.548 ± 0.789
3.143ArgVal: 3.143 ± 0.492
1.019ArgTrp: 1.019 ± 0.261
2.293ArgTyr: 2.293 ± 0.527
0.0ArgXaa: 0.0 ± 0.0
Ser
3.312SerAla: 3.312 ± 0.664
0.51SerCys: 0.51 ± 0.245
4.332SerAsp: 4.332 ± 0.698
4.332SerGlu: 4.332 ± 0.518
2.888SerPhe: 2.888 ± 0.505
4.417SerGly: 4.417 ± 0.557
0.679SerHis: 0.679 ± 0.221
4.841SerIle: 4.841 ± 0.505
4.162SerLys: 4.162 ± 0.552
4.756SerLeu: 4.756 ± 0.625
1.444SerMet: 1.444 ± 0.298
4.926SerAsn: 4.926 ± 0.608
2.293SerPro: 2.293 ± 0.405
2.633SerGln: 2.633 ± 0.517
2.718SerArg: 2.718 ± 0.517
3.652SerSer: 3.652 ± 0.574
3.227SerThr: 3.227 ± 0.486
5.096SerVal: 5.096 ± 0.687
0.595SerTrp: 0.595 ± 0.235
1.869SerTyr: 1.869 ± 0.464
0.0SerXaa: 0.0 ± 0.0
Thr
4.417ThrAla: 4.417 ± 0.849
0.425ThrCys: 0.425 ± 0.193
3.907ThrAsp: 3.907 ± 0.768
3.397ThrGlu: 3.397 ± 0.542
2.718ThrPhe: 2.718 ± 0.509
3.652ThrGly: 3.652 ± 0.483
1.359ThrHis: 1.359 ± 0.36
4.162ThrIle: 4.162 ± 0.747
4.756ThrLys: 4.756 ± 0.604
6.37ThrLeu: 6.37 ± 0.66
0.849ThrMet: 0.849 ± 0.302
4.077ThrAsn: 4.077 ± 0.514
1.444ThrPro: 1.444 ± 0.384
2.378ThrGln: 2.378 ± 0.513
1.953ThrArg: 1.953 ± 0.412
3.992ThrSer: 3.992 ± 0.491
2.888ThrThr: 2.888 ± 0.493
3.992ThrVal: 3.992 ± 0.451
0.934ThrTrp: 0.934 ± 0.281
3.227ThrTyr: 3.227 ± 0.492
0.0ThrXaa: 0.0 ± 0.0
Val
3.737ValAla: 3.737 ± 0.726
0.51ValCys: 0.51 ± 0.24
5.096ValAsp: 5.096 ± 0.688
3.482ValGlu: 3.482 ± 0.633
2.293ValPhe: 2.293 ± 0.436
4.247ValGly: 4.247 ± 0.4
0.934ValHis: 0.934 ± 0.238
4.162ValIle: 4.162 ± 0.517
5.351ValLys: 5.351 ± 0.735
3.992ValLeu: 3.992 ± 0.697
1.189ValMet: 1.189 ± 0.301
2.718ValAsn: 2.718 ± 0.543
1.699ValPro: 1.699 ± 0.369
1.529ValGln: 1.529 ± 0.406
2.718ValArg: 2.718 ± 0.536
4.756ValSer: 4.756 ± 0.637
4.671ValThr: 4.671 ± 0.736
4.332ValVal: 4.332 ± 0.722
0.934ValTrp: 0.934 ± 0.221
2.038ValTyr: 2.038 ± 0.467
0.0ValXaa: 0.0 ± 0.0
Trp
0.595TrpAla: 0.595 ± 0.289
0.085TrpCys: 0.085 ± 0.079
1.274TrpAsp: 1.274 ± 0.439
1.019TrpGlu: 1.019 ± 0.235
0.934TrpPhe: 0.934 ± 0.264
0.51TrpGly: 0.51 ± 0.186
0.34TrpHis: 0.34 ± 0.178
1.104TrpIle: 1.104 ± 0.268
1.019TrpLys: 1.019 ± 0.372
1.444TrpLeu: 1.444 ± 0.382
0.085TrpMet: 0.085 ± 0.077
0.764TrpAsn: 0.764 ± 0.224
0.17TrpPro: 0.17 ± 0.133
0.849TrpGln: 0.849 ± 0.194
0.679TrpArg: 0.679 ± 0.182
1.614TrpSer: 1.614 ± 0.499
0.764TrpThr: 0.764 ± 0.242
1.104TrpVal: 1.104 ± 0.246
0.255TrpTrp: 0.255 ± 0.118
0.255TrpTyr: 0.255 ± 0.138
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.718TyrAla: 2.718 ± 0.494
0.764TyrCys: 0.764 ± 0.361
2.293TyrAsp: 2.293 ± 0.43
3.227TyrGlu: 3.227 ± 0.585
1.529TyrPhe: 1.529 ± 0.347
1.869TyrGly: 1.869 ± 0.473
1.189TyrHis: 1.189 ± 0.312
2.888TyrIle: 2.888 ± 0.524
3.312TyrLys: 3.312 ± 0.606
3.397TyrLeu: 3.397 ± 0.382
0.849TyrMet: 0.849 ± 0.248
1.359TyrAsn: 1.359 ± 0.262
1.189TyrPro: 1.189 ± 0.347
2.123TyrGln: 2.123 ± 0.326
2.293TyrArg: 2.293 ± 0.542
2.038TyrSer: 2.038 ± 0.532
2.293TyrThr: 2.293 ± 0.464
2.633TyrVal: 2.633 ± 0.5
0.085TyrTrp: 0.085 ± 0.079
2.208TyrTyr: 2.208 ± 0.363
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (11775 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski