Sphingomonas lacunae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2780 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M4AXP7|A0A6M4AXP7_9SPHN DUF465 domain-containing protein OS=Sphingomonas lacunae OX=2698828 GN=GV829_03355 PE=4 SV=1
MM1 pKa = 7.34AAAAFAFIAVASPAAAQNAGLTANSGEE28 pKa = 4.02ITLNAGFTPDD38 pKa = 3.76PYY40 pKa = 10.57RR41 pKa = 11.84VSLVSGGSIDD51 pKa = 4.85GGALPGACVGMISDD65 pKa = 4.22APDD68 pKa = 3.44FEE70 pKa = 4.73VTYY73 pKa = 10.9SGGSLPLTFRR83 pKa = 11.84TQSSGDD89 pKa = 3.14TTLIINGPDD98 pKa = 3.63GNWYY102 pKa = 10.44CDD104 pKa = 3.71DD105 pKa = 4.66DD106 pKa = 5.0SGGGVNARR114 pKa = 11.84VTFRR118 pKa = 11.84NARR121 pKa = 11.84AGTYY125 pKa = 9.36DD126 pKa = 2.79IWVGAIGGSAATTLLISEE144 pKa = 4.89LPP146 pKa = 3.55

Molecular weight: 14.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M4AXA6|A0A6M4AXA6_9SPHN RsmB/NOP family class I SAM-dependent RNA methyltransferase OS=Sphingomonas lacunae OX=2698828 GN=GV829_12985 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.45GFRR19 pKa = 11.84ARR21 pKa = 11.84TATVGGRR28 pKa = 11.84AVLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84SKK41 pKa = 11.12LSAA44 pKa = 3.81

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2780

0

2780

898970

36

1699

323.4

34.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.294 ± 0.062

0.813 ± 0.014

5.999 ± 0.035

5.248 ± 0.04

3.502 ± 0.026

9.083 ± 0.04

2.044 ± 0.023

5.178 ± 0.03

2.505 ± 0.036

9.768 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.641 ± 0.025

2.627 ± 0.032

5.331 ± 0.035

3.159 ± 0.022

7.328 ± 0.048

5.419 ± 0.032

5.261 ± 0.035

7.168 ± 0.032

1.534 ± 0.022

2.097 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski